Critical comparison of Giardia duodenalis from Australia and Switzerland using isoenzyme electrophoresis

B. P. Meloni; R. C. A. Thompson; A. M. Strandén; P. Köhler; J. Eckert

doi:10.1016/0001-706X(91)90004-4

Critical comparison of Giardia duodenalis from Australia and Switzerland using isoenzyme electrophoresis

B. P. Meloni, R. C. A. Thompson, A. M. Strandén, P. Köhler, J. Eckert

Research output: Contribution to journal › Article › peer-review

23 Citations (Scopus)

Abstract

Isoenzyme electrophoresis using 13 enzyme systems was applied to 31 Australian and 7 Swiss isolates of Giardia of human, cat, cattle, dog, sheep and rat origin. The Portland (ATCC No. 30888) reference strain was also included. The 39 isolates were divided into 22 different zymodemes. These consisted of 19 zymodemes containing the P1 and Australian isloates and three zymodemes containing Swiss isolates only. Differences in enzyme profiles between zymodemes was measured by euclidean distance and it was found that Australian isolates of Giardia exhibited more variation than the Swiss isolates. Relationships between zymodemes determined by clustering analysis are discussed with particular reference to the zoonotic potential of Giardia.

Original language	English
Pages (from-to)	115-124
Number of pages	10
Journal	Acta Tropica
Volume	50
Issue number	2
DOIs	https://doi.org/10.1016/0001-706X(91)90004-4
Publication status	Published - Dec 1991
Externally published	Yes

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10.1016/0001-706X(91)90004-4

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title = "Critical comparison of Giardia duodenalis from Australia and Switzerland using isoenzyme electrophoresis",

abstract = "Isoenzyme electrophoresis using 13 enzyme systems was applied to 31 Australian and 7 Swiss isolates of Giardia of human, cat, cattle, dog, sheep and rat origin. The Portland (ATCC No. 30888) reference strain was also included. The 39 isolates were divided into 22 different zymodemes. These consisted of 19 zymodemes containing the P1 and Australian isloates and three zymodemes containing Swiss isolates only. Differences in enzyme profiles between zymodemes was measured by euclidean distance and it was found that Australian isolates of Giardia exhibited more variation than the Swiss isolates. Relationships between zymodemes determined by clustering analysis are discussed with particular reference to the zoonotic potential of Giardia.",

keywords = "Genetic variation, Giardia, Isoenzyme electrophoresis, Zoonosis",

author = "Meloni, {B. P.} and Thompson, {R. C. A.} and Strand{\'e}n, {A. M.} and P. K{\"o}hler and J. Eckert",

note = "Funding Information: This study was supported by the National Health and Medical Research Council of Australia and the Swiss National Science Foundation (Grant No. 3.856-086). The authors with to thank Val Wymer for supplying the human Giardia samples and Russel Hobbs for running the TAXAN 2 computer package.",

year = "1991",

month = dec,

doi = "10.1016/0001-706X(91)90004-4",

language = "English",

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journal = "Acta Tropica",

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T1 - Critical comparison of Giardia duodenalis from Australia and Switzerland using isoenzyme electrophoresis

AU - Meloni, B. P.

AU - Thompson, R. C. A.

AU - Strandén, A. M.

AU - Köhler, P.

AU - Eckert, J.

N1 - Funding Information: This study was supported by the National Health and Medical Research Council of Australia and the Swiss National Science Foundation (Grant No. 3.856-086). The authors with to thank Val Wymer for supplying the human Giardia samples and Russel Hobbs for running the TAXAN 2 computer package.

PY - 1991/12

Y1 - 1991/12

N2 - Isoenzyme electrophoresis using 13 enzyme systems was applied to 31 Australian and 7 Swiss isolates of Giardia of human, cat, cattle, dog, sheep and rat origin. The Portland (ATCC No. 30888) reference strain was also included. The 39 isolates were divided into 22 different zymodemes. These consisted of 19 zymodemes containing the P1 and Australian isloates and three zymodemes containing Swiss isolates only. Differences in enzyme profiles between zymodemes was measured by euclidean distance and it was found that Australian isolates of Giardia exhibited more variation than the Swiss isolates. Relationships between zymodemes determined by clustering analysis are discussed with particular reference to the zoonotic potential of Giardia.

AB - Isoenzyme electrophoresis using 13 enzyme systems was applied to 31 Australian and 7 Swiss isolates of Giardia of human, cat, cattle, dog, sheep and rat origin. The Portland (ATCC No. 30888) reference strain was also included. The 39 isolates were divided into 22 different zymodemes. These consisted of 19 zymodemes containing the P1 and Australian isloates and three zymodemes containing Swiss isolates only. Differences in enzyme profiles between zymodemes was measured by euclidean distance and it was found that Australian isolates of Giardia exhibited more variation than the Swiss isolates. Relationships between zymodemes determined by clustering analysis are discussed with particular reference to the zoonotic potential of Giardia.

KW - Genetic variation

KW - Giardia

KW - Isoenzyme electrophoresis

KW - Zoonosis

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VL - 50

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EP - 124

JO - Acta Tropica

JF - Acta Tropica

IS - 2

ER -

Critical comparison of Giardia duodenalis from Australia and Switzerland using isoenzyme electrophoresis

Abstract

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