CRISPR-Based Genome Editing Tools: An Accelerator in Crop Breeding for a Changing Future

Fangning Zhang, Ting Xiang Neik, William J.W. Thomas, Jacqueline Batley

Research output: Contribution to journalReview articlepeer-review


Genome editing is an important strategy to maintain global food security and achieve sustainable agricultural development. Among all genome editing tools, CRISPR-Cas is currently the most prevalent and offers the most promise. In this review, we summarize the development of CRISPR-Cas systems, outline their classification and distinctive features, delineate their natural mechanisms in plant genome editing and exemplify the applications in plant research. Both classical and recently discovered CRISPR-Cas systems are included, detailing the class, type, structures and functions of each. We conclude by highlighting the challenges that come with CRISPR-Cas and offer suggestions on how to tackle them. We believe the gene editing toolbox will be greatly enriched, providing new avenues for a more efficient and precise breeding of climate-resilient crops.

Original languageEnglish
Article number8623
Number of pages27
JournalInternational Journal of Molecular Sciences
Issue number10
Publication statusPublished - 11 May 2023


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