Crambescidin 800, isolated from the marine sponge monanchora viridis, induces cell cycle arrest and apoptosis in triple-negative breast cancer cells

Sumi Shrestha, Anabel Sorolla, Jane Fromont, Pilar Blancafort, Gavin R. Flematti

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Triple negative breast cancer (TNBC) is currently the only group of breast cancers without an effective targeted therapy. Marine sponges have historically been a source of compounds with anticancer activity. In this study, we screened extracts from twenty marine sponges collected off the coast of Western Australia for cytotoxic activity against TNBC cells. One very active extract derived from the sponge Monanchora viridis was selected for bioactivity-guided fractionation. Through multiple steps of purification, we isolated a potent cytotoxic compound, which was identified as crambescidin 800 (C800). We found that C800 exhibited cytotoxic potency in a panel of breast cancer cells, of which TNBC and luminal cancer cell models were the most sensitive. In addition, C800 induced cell cycle arrest at the G2/M phase, resulting in a decline in the expression of cyclin D1, CDK4, and CDK6 in TNBC cells. This effect was associated with the inhibition of phosphorylation of Akt, NF-κB, and MAPK pathways, resulting in apoptosis in TNBC cells.

Original languageEnglish
Article number53
JournalMarine Drugs
Volume16
Issue number2
DOIs
Publication statusPublished - 1 Feb 2018

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