CpG island-mediated global gene regulatory modes in mouse embryonic stem cells

S. Beck, B. Lee, C. Rhee, J. Song, Andrew Woo, J. Kim

    Research output: Contribution to journalArticle

    14 Citations (Scopus)

    Abstract

    © 2014 Macmillan Publishers Limited. Both transcriptional and epigenetic regulations are fundamental for the control of eukaryotic gene expression. Here we perform a compendium analysis of >200 large sequencing data sets to elucidate the regulatory logic of global gene expression programs in mouse embryonic stem (ES) cells. We define four major classes of DNA-binding proteins (Core, PRC, MYC and CTCF) based on their target co-occupancy, and discover reciprocal regulation between the MYC and PRC classes for the activity of nearly all genes under the control of the CpG island (CGI)-containing promoters. This CGI-dependent regulatory mode explains the functional segregation between CGI-containing and CGI-less genes during early development. By defining active enhancers based on the co-occupancy of the Core class, we further demonstrate their additive roles in CGI-containing gene expression and cell type-specific roles in CGI-less gene expression. Altogether, our analyses provide novel insights into previously unknown CGI-dependent global gene regulatory modes.
    Original languageEnglish
    Pages (from-to)12pp
    JournalNature Communications
    Volume5
    DOIs
    Publication statusPublished - 2014

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