TY - JOUR
T1 - Correlation of probiotic Lactobacillus salivarius growth phase with its cell wall-associated proteome
AU - Kelly, Peter
AU - Maguire, Patricia B.
AU - Bennett, Mary
AU - Fitzgerald, Desmond J.
AU - Edwards, Richard J.
AU - Thiede, Bernd
AU - Treumann, Achim
AU - Collins, J. Kevin
AU - O'Sullivan, Gerald C.
AU - Shanahan, Fergus
AU - Dunne, Colum
N1 - Funding Information:
The authors thank Maurice O’Donoghue for technical assistance. This study was supported in part by grant aid under the Food Sub-Programme of the Operational Programme for Industrial Development administered by the Irish Department of Agriculture and Food, part-financed by the European Regional Development Fund; the Programme for Research in Third Level Institutions (PRTLI) administered by the Irish Higher Education Authority; the Irish Health Research Board; Science Foundation Ireland; and the European Commission (PROBDEMO: FAIR-CT96-1028; PROGID: QLK1-2000-00563).
PY - 2005/11/1
Y1 - 2005/11/1
N2 - Lactobacillus salivarius subsp. salivarius UCC118 is a probiotic bacterium that was originally isolated from human intestinal tissues and was subsequently shown in a pilot study to alleviate symptoms associated with mild-moderate Crohn's disease. Strain UCC118 can adhere to animal and human intestinal tissue, and to both healthy and inflamed ulcerative colitis mucosa, irrespective of location in the gut. In this study, an enzymatic technique has been combined with proteomic analysis to correlate bacterial growth phase with the presence of factors present in the cell wall of the bacterium. Using PAGE electrophoresis, it was determined that progression from lag to log to stationary growth phases in vitro correlated with increasing prominence of an 84 kD protein associated with in vitro adherence ability. Isolated proteins from the 84 kD band region were further separated by two-dimensional electrophoresis, resolving this band into 20 individual protein spots at differing isoelectric points. The protein moieties were excised, trypsin digested and subjected to tandem mass spectrometry. The observed proteins are analogous to those reported to be associated with the Listeria monocytogenes cell-wall proteome, and include DnaK, Ef-Ts and pyruvate kinase. These data suggest that at least some of the beneficial attributes of probiotic lactobacilli, and in particular this strain, may be due to nonpathogenic mimicry of pathogens and potentially be mediated through a form of attenuated virulence.
AB - Lactobacillus salivarius subsp. salivarius UCC118 is a probiotic bacterium that was originally isolated from human intestinal tissues and was subsequently shown in a pilot study to alleviate symptoms associated with mild-moderate Crohn's disease. Strain UCC118 can adhere to animal and human intestinal tissue, and to both healthy and inflamed ulcerative colitis mucosa, irrespective of location in the gut. In this study, an enzymatic technique has been combined with proteomic analysis to correlate bacterial growth phase with the presence of factors present in the cell wall of the bacterium. Using PAGE electrophoresis, it was determined that progression from lag to log to stationary growth phases in vitro correlated with increasing prominence of an 84 kD protein associated with in vitro adherence ability. Isolated proteins from the 84 kD band region were further separated by two-dimensional electrophoresis, resolving this band into 20 individual protein spots at differing isoelectric points. The protein moieties were excised, trypsin digested and subjected to tandem mass spectrometry. The observed proteins are analogous to those reported to be associated with the Listeria monocytogenes cell-wall proteome, and include DnaK, Ef-Ts and pyruvate kinase. These data suggest that at least some of the beneficial attributes of probiotic lactobacilli, and in particular this strain, may be due to nonpathogenic mimicry of pathogens and potentially be mediated through a form of attenuated virulence.
KW - Growth phase
KW - Lactobacillus
KW - Mass spectrometry
KW - Probiotic
KW - Proteomics
UR - http://www.scopus.com/inward/record.url?scp=26844454554&partnerID=8YFLogxK
U2 - 10.1016/j.femsle.2005.08.051
DO - 10.1016/j.femsle.2005.08.051
M3 - Article
C2 - 16214296
AN - SCOPUS:26844454554
SN - 0378-1097
VL - 252
SP - 153
EP - 159
JO - FEMS Microbiology Letters
JF - FEMS Microbiology Letters
IS - 1
ER -