Correlation and agreement between the number of Class I and II eplet mismatches calculated using serological, low-intermediate and high resolution molecular human leukocyte antigen typing methods

S. Fidler, L. D'Orsogna, A. B. Irish, J. Lewis, G. Wong, W. H. Lim

Research output: Contribution to journalAbstract/Meeting Abstract

Abstract

Aim: To evaluate the agreement between eplet mismatches calculated by serological and two-digit typing methods compared to high-resolution four-digit typing method. Background: Structural human leukocyte antigen (HLA) matching at the eplet level can be identified by HLAMatchmaker, but requires the entry of four-digit allele typing, which are often not practicable in donor kidney allocation because of time constraint. Methods: In a cohort of 264 donor/recipient pairs, the evaluation of measurement error was assessed using intra-class correlation to confirm the absolute agreement between the number of eplet mismatches at class I (HLA-A, -B, C) and II loci (HLA-DQ and -DR) calculated using serological or two-digit molecular typing compared to four-digit molecular typing methods. The proportion of donor/recipient pairs with a difference of >5 eplet mismatches between the HLA typing methods was also determined. Results: Intra-class correlation coefficients between serological and four-digit molecular typing methods were 0.969 (95% confidence intervals [95%CI] 0.960-0.975) and 0.926 (95%CI 0.899-0.944), respectively; and 0.995 (95%CI 0.994-0.996) and 0.993 (95%CI 0.991-0.995), respectively between two-digit and four-digit molecular typing methods. The proportion of donor/recipient pairs with a difference of >5 eplet mismatches at class I and II loci was 4% and 16% for serological versus four-digit molecular typing methods, and <1% and 2% for two-digit versus four-digit molecular typing methods, respectively. Conclusions: Compared with serology, there is a high level of agreement in the number of eplet mismatches calculated using two- compared to four-digit molecular HLA-typing methods, suggesting that two-digit typing may be sufficient in determining eplet mismatch load in kidney transplantation.
Original languageEnglish
Pages (from-to)91-91
Number of pages1
JournalNephrology
Volume22
Publication statusPublished - Sep 2017
Externally publishedYes

Cite this

@article{dbeb248d31414567b418149adec5fc93,
title = "Correlation and agreement between the number of Class I and II eplet mismatches calculated using serological, low-intermediate and high resolution molecular human leukocyte antigen typing methods",
abstract = "Aim: To evaluate the agreement between eplet mismatches calculated by serological and two-digit typing methods compared to high-resolution four-digit typing method. Background: Structural human leukocyte antigen (HLA) matching at the eplet level can be identified by HLAMatchmaker, but requires the entry of four-digit allele typing, which are often not practicable in donor kidney allocation because of time constraint. Methods: In a cohort of 264 donor/recipient pairs, the evaluation of measurement error was assessed using intra-class correlation to confirm the absolute agreement between the number of eplet mismatches at class I (HLA-A, -B, C) and II loci (HLA-DQ and -DR) calculated using serological or two-digit molecular typing compared to four-digit molecular typing methods. The proportion of donor/recipient pairs with a difference of >5 eplet mismatches between the HLA typing methods was also determined. Results: Intra-class correlation coefficients between serological and four-digit molecular typing methods were 0.969 (95{\%} confidence intervals [95{\%}CI] 0.960-0.975) and 0.926 (95{\%}CI 0.899-0.944), respectively; and 0.995 (95{\%}CI 0.994-0.996) and 0.993 (95{\%}CI 0.991-0.995), respectively between two-digit and four-digit molecular typing methods. The proportion of donor/recipient pairs with a difference of >5 eplet mismatches at class I and II loci was 4{\%} and 16{\%} for serological versus four-digit molecular typing methods, and <1{\%} and 2{\%} for two-digit versus four-digit molecular typing methods, respectively. Conclusions: Compared with serology, there is a high level of agreement in the number of eplet mismatches calculated using two- compared to four-digit molecular HLA-typing methods, suggesting that two-digit typing may be sufficient in determining eplet mismatch load in kidney transplantation.",
author = "S. Fidler and L. D'Orsogna and Irish, {A. B.} and J. Lewis and G. Wong and Lim, {W. H.}",
year = "2017",
month = "9",
language = "English",
volume = "22",
pages = "91--91",
journal = "Nephrology (Carlton, Vic.)",
issn = "1320-5358",
publisher = "John Wiley & Sons",

}

TY - JOUR

T1 - Correlation and agreement between the number of Class I and II eplet mismatches calculated using serological, low-intermediate and high resolution molecular human leukocyte antigen typing methods

AU - Fidler, S.

AU - D'Orsogna, L.

AU - Irish, A. B.

AU - Lewis, J.

AU - Wong, G.

AU - Lim, W. H.

PY - 2017/9

Y1 - 2017/9

N2 - Aim: To evaluate the agreement between eplet mismatches calculated by serological and two-digit typing methods compared to high-resolution four-digit typing method. Background: Structural human leukocyte antigen (HLA) matching at the eplet level can be identified by HLAMatchmaker, but requires the entry of four-digit allele typing, which are often not practicable in donor kidney allocation because of time constraint. Methods: In a cohort of 264 donor/recipient pairs, the evaluation of measurement error was assessed using intra-class correlation to confirm the absolute agreement between the number of eplet mismatches at class I (HLA-A, -B, C) and II loci (HLA-DQ and -DR) calculated using serological or two-digit molecular typing compared to four-digit molecular typing methods. The proportion of donor/recipient pairs with a difference of >5 eplet mismatches between the HLA typing methods was also determined. Results: Intra-class correlation coefficients between serological and four-digit molecular typing methods were 0.969 (95% confidence intervals [95%CI] 0.960-0.975) and 0.926 (95%CI 0.899-0.944), respectively; and 0.995 (95%CI 0.994-0.996) and 0.993 (95%CI 0.991-0.995), respectively between two-digit and four-digit molecular typing methods. The proportion of donor/recipient pairs with a difference of >5 eplet mismatches at class I and II loci was 4% and 16% for serological versus four-digit molecular typing methods, and <1% and 2% for two-digit versus four-digit molecular typing methods, respectively. Conclusions: Compared with serology, there is a high level of agreement in the number of eplet mismatches calculated using two- compared to four-digit molecular HLA-typing methods, suggesting that two-digit typing may be sufficient in determining eplet mismatch load in kidney transplantation.

AB - Aim: To evaluate the agreement between eplet mismatches calculated by serological and two-digit typing methods compared to high-resolution four-digit typing method. Background: Structural human leukocyte antigen (HLA) matching at the eplet level can be identified by HLAMatchmaker, but requires the entry of four-digit allele typing, which are often not practicable in donor kidney allocation because of time constraint. Methods: In a cohort of 264 donor/recipient pairs, the evaluation of measurement error was assessed using intra-class correlation to confirm the absolute agreement between the number of eplet mismatches at class I (HLA-A, -B, C) and II loci (HLA-DQ and -DR) calculated using serological or two-digit molecular typing compared to four-digit molecular typing methods. The proportion of donor/recipient pairs with a difference of >5 eplet mismatches between the HLA typing methods was also determined. Results: Intra-class correlation coefficients between serological and four-digit molecular typing methods were 0.969 (95% confidence intervals [95%CI] 0.960-0.975) and 0.926 (95%CI 0.899-0.944), respectively; and 0.995 (95%CI 0.994-0.996) and 0.993 (95%CI 0.991-0.995), respectively between two-digit and four-digit molecular typing methods. The proportion of donor/recipient pairs with a difference of >5 eplet mismatches at class I and II loci was 4% and 16% for serological versus four-digit molecular typing methods, and <1% and 2% for two-digit versus four-digit molecular typing methods, respectively. Conclusions: Compared with serology, there is a high level of agreement in the number of eplet mismatches calculated using two- compared to four-digit molecular HLA-typing methods, suggesting that two-digit typing may be sufficient in determining eplet mismatch load in kidney transplantation.

UR - http://www.anzsnasm.com/3102

M3 - Abstract/Meeting Abstract

VL - 22

SP - 91

EP - 91

JO - Nephrology (Carlton, Vic.)

JF - Nephrology (Carlton, Vic.)

SN - 1320-5358

ER -