TY - JOUR
T1 - Convergence of 3D printed biomimetic wound dressings and adult stem cell therapy
AU - Shafiee, Abbas
AU - Cavalcanti, Amanda S.
AU - Saidy, Navid T.
AU - Schneidereit, Dominik
AU - Friedrich, Oliver
AU - Ravichandran, Akhilandeshwari
AU - De-Juan-Pardo, Elena M.
AU - Hutmacher, Dietmar W.
PY - 2021/1
Y1 - 2021/1
N2 - Biomimetically designed medical-grade polycaprolactone (mPCL) dressings are 3D-printed with pore architecture and anisotropic mechanical characteristics that favor skin wound healing with reduced scarring. Melt electrowritten mPCL dressings are seeded with human gingival tissue multipotent mesenchymal stem/stromal cells and cryopreserved using a clinically approved method. The regenerative potential of fresh or frozen cell-seeded mPCL dressing is compared in a splinted full-thickness excisional wound in a rat model over six weeks. The application of 3D-printed mPCL dressings decreased wound contracture and significantly improved skin regeneration through granulation and re-epithelialization compared to control groups. Combining 3D-printed biomimetic wound dressings and precursor cell delivery enhances physiological wound closure with reduced scar tissue formation.
AB - Biomimetically designed medical-grade polycaprolactone (mPCL) dressings are 3D-printed with pore architecture and anisotropic mechanical characteristics that favor skin wound healing with reduced scarring. Melt electrowritten mPCL dressings are seeded with human gingival tissue multipotent mesenchymal stem/stromal cells and cryopreserved using a clinically approved method. The regenerative potential of fresh or frozen cell-seeded mPCL dressing is compared in a splinted full-thickness excisional wound in a rat model over six weeks. The application of 3D-printed mPCL dressings decreased wound contracture and significantly improved skin regeneration through granulation and re-epithelialization compared to control groups. Combining 3D-printed biomimetic wound dressings and precursor cell delivery enhances physiological wound closure with reduced scar tissue formation.
KW - 3D printing
KW - Cryopreservation
KW - Regenerative medicine
KW - Tissue Engineering
KW - Wound care
UR - http://www.scopus.com/inward/record.url?scp=85097345280&partnerID=8YFLogxK
U2 - 10.1016/j.biomaterials.2020.120558
DO - 10.1016/j.biomaterials.2020.120558
M3 - Article
C2 - 33307369
AN - SCOPUS:85097345280
SN - 0142-9612
VL - 268
JO - Biomaterials
JF - Biomaterials
M1 - 120558
ER -