Contrasting effects of interferon-gamma and interleukin-4 on the interleukin-6 activity of stimulated human monocytes

D. L. Cheung; P. H. Hart; G. F. Vitti; G. A. Whitty; J. A. Hamilton

Contrasting effects of interferon-gamma and interleukin-4 on the interleukin-6 activity of stimulated human monocytes

D. L. Cheung, P. H. Hart, G. F. Vitti, G. A. Whitty, J. A. Hamilton

Research output: Contribution to journal › Article › peer-review

Abstract

Stimulated human monocytes/macrophages are a source of interleukin-6 (IL-6), which is a likely mediator involved in immune and inflammatory reactions. The means to control production of IL-6 by these cells could therefore have therapeutic applications. We report here, for lipopolysaccharide (LPS)-stimulated human monocytes in vitro, that the lymphokine, interferon-gamma (IFN-γ) (100 U/ml), enhanced the level of IL-6 activity, whereas another lymphokine, interleukin-4 (IL-4) (≥0.1 U/ml; ≥1.2 x 10^-11M), suppressed it. The effect of the two lymphokines were manifested at the level of mRNA. The action of the IL-4 was similar to that of the glucocorticoid, dexamethasone, but observed at a lower molar concentration. Such regulation of monocyte IL-6 activity is similar to that found previously for interleukin-1 (IL-1) and tumour necrosis factor-alpha (TNF-α) synthesis.

Original language	English
Pages (from-to)	70-75
Number of pages	6
Journal	Immunology
Volume	71
Issue number	1
Publication status	Published - 1 Jan 1990
Externally published	Yes

Cite this

@article{94f327fb758e47aa8276af0ad5e66f92,

title = "Contrasting effects of interferon-gamma and interleukin-4 on the interleukin-6 activity of stimulated human monocytes",

abstract = "Stimulated human monocytes/macrophages are a source of interleukin-6 (IL-6), which is a likely mediator involved in immune and inflammatory reactions. The means to control production of IL-6 by these cells could therefore have therapeutic applications. We report here, for lipopolysaccharide (LPS)-stimulated human monocytes in vitro, that the lymphokine, interferon-gamma (IFN-γ) (100 U/ml), enhanced the level of IL-6 activity, whereas another lymphokine, interleukin-4 (IL-4) (≥0.1 U/ml; ≥1.2 x 10-11M), suppressed it. The effect of the two lymphokines were manifested at the level of mRNA. The action of the IL-4 was similar to that of the glucocorticoid, dexamethasone, but observed at a lower molar concentration. Such regulation of monocyte IL-6 activity is similar to that found previously for interleukin-1 (IL-1) and tumour necrosis factor-alpha (TNF-α) synthesis.",

author = "Cheung, {D. L.} and Hart, {P. H.} and Vitti, {G. F.} and Whitty, {G. A.} and Hamilton, {J. A.}",

year = "1990",

month = jan,

day = "1",

language = "English",

volume = "71",

pages = "70--75",

journal = "Immunology",

issn = "0019-2805",

publisher = "Wiley-Blackwell",

number = "1",

}

TY - JOUR

T1 - Contrasting effects of interferon-gamma and interleukin-4 on the interleukin-6 activity of stimulated human monocytes

AU - Cheung, D. L.

AU - Hart, P. H.

AU - Vitti, G. F.

AU - Whitty, G. A.

AU - Hamilton, J. A.

PY - 1990/1/1

Y1 - 1990/1/1

N2 - Stimulated human monocytes/macrophages are a source of interleukin-6 (IL-6), which is a likely mediator involved in immune and inflammatory reactions. The means to control production of IL-6 by these cells could therefore have therapeutic applications. We report here, for lipopolysaccharide (LPS)-stimulated human monocytes in vitro, that the lymphokine, interferon-gamma (IFN-γ) (100 U/ml), enhanced the level of IL-6 activity, whereas another lymphokine, interleukin-4 (IL-4) (≥0.1 U/ml; ≥1.2 x 10-11M), suppressed it. The effect of the two lymphokines were manifested at the level of mRNA. The action of the IL-4 was similar to that of the glucocorticoid, dexamethasone, but observed at a lower molar concentration. Such regulation of monocyte IL-6 activity is similar to that found previously for interleukin-1 (IL-1) and tumour necrosis factor-alpha (TNF-α) synthesis.

AB - Stimulated human monocytes/macrophages are a source of interleukin-6 (IL-6), which is a likely mediator involved in immune and inflammatory reactions. The means to control production of IL-6 by these cells could therefore have therapeutic applications. We report here, for lipopolysaccharide (LPS)-stimulated human monocytes in vitro, that the lymphokine, interferon-gamma (IFN-γ) (100 U/ml), enhanced the level of IL-6 activity, whereas another lymphokine, interleukin-4 (IL-4) (≥0.1 U/ml; ≥1.2 x 10-11M), suppressed it. The effect of the two lymphokines were manifested at the level of mRNA. The action of the IL-4 was similar to that of the glucocorticoid, dexamethasone, but observed at a lower molar concentration. Such regulation of monocyte IL-6 activity is similar to that found previously for interleukin-1 (IL-1) and tumour necrosis factor-alpha (TNF-α) synthesis.

UR - http://www.scopus.com/inward/record.url?scp=0025041133&partnerID=8YFLogxK

M3 - Article

C2 - 2120129

AN - SCOPUS:0025041133

SN - 0019-2805

VL - 71

SP - 70

EP - 75

JO - Immunology

JF - Immunology

IS - 1

ER -

Contrasting effects of interferon-gamma and interleukin-4 on the interleukin-6 activity of stimulated human monocytes

Abstract

Other files and links

Fingerprint

Cite this