Contrast and depth enhancement in two-photon microscopy of human skin ex vivo by use of optical clearing agents

R. Cicchi, F.S. Pavone, D. Massi, David Sampson

    Research output: Contribution to journalArticlepeer-review

    117 Citations (Scopus)

    Abstract

    We investigate the application of hyperosmotic optical clearing agents to improve the image contrast and penetration depth in two-photon microscopy of human dermis ex vivo. We show that the agents glycerol, propylene glycol, and glucose all convey significant improvements and we provide results on their dynamic behaviour and the reversibility of the effect. At suitable concentrations, such agents have the potential to be compatible with living tissue and may possibly enhance in-vivo deep-tissue imaging. (C) 2005 Optical Society of America.
    Original languageEnglish
    Pages (from-to)2337-2344
    JournalOptics Express
    Volume13
    Issue number7
    DOIs
    Publication statusPublished - 2005

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