Contrast and depth enhancement in two-photon microscopy of human skin ex vivo by use of optical clearing agents

R. Cicchi; F.S. Pavone; D. Massi; David Sampson

doi:10.1364/OPEX.13.002337

Contrast and depth enhancement in two-photon microscopy of human skin ex vivo by use of optical clearing agents

R. Cicchi, F.S. Pavone, D. Massi, David Sampson

Research output: Contribution to journal › Article › peer-review

120 Citations (Scopus)

Abstract

We investigate the application of hyperosmotic optical clearing agents to improve the image contrast and penetration depth in two-photon microscopy of human dermis ex vivo. We show that the agents glycerol, propylene glycol, and glucose all convey significant improvements and we provide results on their dynamic behaviour and the reversibility of the effect. At suitable concentrations, such agents have the potential to be compatible with living tissue and may possibly enhance in-vivo deep-tissue imaging. (C) 2005 Optical Society of America.

Original language	English
Pages (from-to)	2337-2344
Journal	Optics Express
Volume	13
Issue number	7
DOIs	https://doi.org/10.1364/OPEX.13.002337
Publication status	Published - 2005

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title = "Contrast and depth enhancement in two-photon microscopy of human skin ex vivo by use of optical clearing agents",

abstract = "We investigate the application of hyperosmotic optical clearing agents to improve the image contrast and penetration depth in two-photon microscopy of human dermis ex vivo. We show that the agents glycerol, propylene glycol, and glucose all convey significant improvements and we provide results on their dynamic behaviour and the reversibility of the effect. At suitable concentrations, such agents have the potential to be compatible with living tissue and may possibly enhance in-vivo deep-tissue imaging. (C) 2005 Optical Society of America.",

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AU - Cicchi, R.

AU - Pavone, F.S.

AU - Massi, D.

AU - Sampson, David

PY - 2005

Y1 - 2005

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AB - We investigate the application of hyperosmotic optical clearing agents to improve the image contrast and penetration depth in two-photon microscopy of human dermis ex vivo. We show that the agents glycerol, propylene glycol, and glucose all convey significant improvements and we provide results on their dynamic behaviour and the reversibility of the effect. At suitable concentrations, such agents have the potential to be compatible with living tissue and may possibly enhance in-vivo deep-tissue imaging. (C) 2005 Optical Society of America.

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DO - 10.1364/OPEX.13.002337

M3 - Article

SN - 1094-4087

VL - 13

SP - 2337

EP - 2344

JO - Optics Express

JF - Optics Express

IS - 7

ER -

Contrast and depth enhancement in two-photon microscopy of human skin ex vivo by use of optical clearing agents

Abstract

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