不同品种苦荞中的4种活性黄酮含量

Translated title of the contribution: Contents of four active flavonoids in different species of buckwheat

Shengcai Pu, Lu Lu, Wei Jiao, Huawu Shao, Xiaocheng Yang

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

In order to examine the merits of buckwheat, four main flavonoids (rutin, kaempferol-3-rutinoside, quercetin and kaempferol) were analyzed by using reversed-phase HPLC in 21 kinds of buckwheat, with chromatography column of Damonsil C18 (4.6 mm × 250 mm, 5 μm), mobile phase of acetonitrile-0.2% acetic acid solution, flow rate of 1.0 mL/min, detection wavelength of 354 nm, and column temperature of 30°C. The results showed an average moisture content of 4.907 3-6.993 6% in 21 kinds of different species of buckwheat seeds at room temperature; under constant weight state, the bran mass fraction was 21.84-30.03%, accounting for 1/4 of buckwheat seeds quality. The linear ranges of rutin, kaempferol-3-rutinoside, quercetin and kaempferol were 20-400 μg/mL, 2-40 μg/mL, 1-20 μg/mL, 0.5-10 μg/mL, respectively. In the powder of 21 kinds of buckwheat seeds, the content ranges of rutin, kaempferol-3-rutinoside, quercetin and kaempferol were 12.137 0-20.318 6 mg/g, 1.478 7-2.871 9 mg/g, 0.014 6-0.901 5 mg/g, 0-0.015 9 mg/g, respectively. The total content of the four main flavonoids was 14.015 1-22.871 7 mg/g. In seeds bran, the content ranges of rutin, kaempferol-3-rutinoside, quercetin and kaempferol were 4.269 0-12.118 9 mg/g, 0.258 5-1.014 9 mg/g, 0.061 8-0.735 4 mg/g, 0-0.026 3 mg/g, respectively. The total content of the four main flavonoids was 4.651 1-13.148 6 mg/g. The results indicated that whether in seed powder or bran, rutin is the flavonoid of the highest contents, followed by kaempferol-3-rutinoside and quercetin, with kaempferol levels the lowest, less than one in ten thousandth. This study provides some important evidences for the use of certain kinds of buckwheat.

Translated title of the contributionContents of four active flavonoids in different species of buckwheat
Original languageChinese
Pages (from-to)470-476
Number of pages7
JournalChinese Journal of Applied and Environmental Biology
Volume21
Issue number3
DOIs
Publication statusPublished - 25 Jun 2015
Externally publishedYes

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