Construction and expression of recombinant human thymosin alpha1 fusion gene in Pichia pastoris

Translated title of the contribution: Construction and expression of recombinant human thymosin alpha1 fusion gene in Pichia pastoris

Wen Huang, Zhao-yu Li, Li-ji Jin, Li-jia An

Research output: Contribution to journalArticle

Abstract

The human Thymosin alpha1 (hTalpha1) gene was synthesized according to the optimal codons of Pichia pastoris and was fused in 5' terminal of ribosomal protein (RP) gene using over lapping polymerase chain reaction. The fusion gene was inserted into expression vector of pPIC3. 5K and was transformed into HIS4 mutant strain GS115 by electroporation. Both SDS-PAGE and Western blot indicated that this fusion protein was expressed. The expression level was about 25mg/L.

Translated title of the contributionConstruction and expression of recombinant human thymosin alpha1 fusion gene in Pichia pastoris
Original languageChinese
Pages (from-to)679-83
Number of pages5
JournalYi chuan = Hereditas
Volume24
Issue number6
Publication statusPublished - Nov 2002

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