Consensus multivariate methods in gas chromatography mass spectrometry and denaturing gradient gel electrophoresis: MHC-congenic and other strains of mice can be classified according to the profiles of volatiles and microflora in their scent-marks

S. Zomer; S.J. Dixon; Y. Xu; S.P. Jensen; H. Wang; C.V. Lanyon; A.G. O'Donnell; A.S. Clare; L.M. Gosling; D.J. Penn; R.G. Brereton

doi:10.1039/b807061j

Consensus multivariate methods in gas chromatography mass spectrometry and denaturing gradient gel electrophoresis: MHC-congenic and other strains of mice can be classified according to the profiles of volatiles and microflora in their scent-marks

S. Zomer, S.J. Dixon, Y. Xu, S.P. Jensen, H. Wang, C.V. Lanyon, A.G. O'Donnell, A.S. Clare, L.M. Gosling, D.J. Penn, R.G. Brereton

Research output: Contribution to journal › Article › peer-review

34 Citations (Scopus)

Abstract

House mice (Mus domesticus) communicate using scent-marks, and the chemical and microbialcomposition of these ‘extended phenotypes’ are both influenced by genetics. This study examined howthe genes of the major histocompatibility complex (MHC) and background genes influence the volatilecompounds (analysed with Gas Chromatography Mass Spectrometry or GC/MS) and microbialcommunities (analysed using Denaturating Gradient Gel Electrophoresis or DGGE) in scent-marksproduced by congenic strains of mice. The use of Consensus Principal Components Analysis isdescribed and shows relationships between the two types of fingerprints (GC/MS and DGGE profiles).Classification methods including Support Vector Machines and Discriminant Partial Least Squaressuggest that mice can be classified according to both background strain and MHC-haplotype. Asexpected, the differences among the mice were much greater between strains that vary at both MHCand background loci than the congenics, which differ only at the MHC. These results indicate that thevolatiles in scent-marks provide information about genetic similarity of the mice, and support the ideathat the production of these genetically determined volatiles is influenced by commensal microflora.This paper describes the application of consensus methods to relate two blocks of analytical data.

Original language	English
Pages (from-to)	114-123
Journal	The Analyst
Volume	134
Issue number	1
DOIs	https://doi.org/10.1039/b807061j
Publication status	Published - 2009

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Zomer, S., Dixon, S. J., Xu, Y., Jensen, S. P., Wang, H., Lanyon, C. V., O'Donnell, A. G., Clare, A. S., Gosling, L. M., Penn, D. J., & Brereton, R. G. (2009). Consensus multivariate methods in gas chromatography mass spectrometry and denaturing gradient gel electrophoresis: MHC-congenic and other strains of mice can be classified according to the profiles of volatiles and microflora in their scent-marks. The Analyst, 134(1), 114-123. https://doi.org/10.1039/b807061j

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title = "Consensus multivariate methods in gas chromatography mass spectrometry and denaturing gradient gel electrophoresis: MHC-congenic and other strains of mice can be classified according to the profiles of volatiles and microflora in their scent-marks",

abstract = "House mice (Mus domesticus) communicate using scent-marks, and the chemical and microbialcomposition of these {\textquoteleft}extended phenotypes{\textquoteright} are both influenced by genetics. This study examined howthe genes of the major histocompatibility complex (MHC) and background genes influence the volatilecompounds (analysed with Gas Chromatography Mass Spectrometry or GC/MS) and microbialcommunities (analysed using Denaturating Gradient Gel Electrophoresis or DGGE) in scent-marksproduced by congenic strains of mice. The use of Consensus Principal Components Analysis isdescribed and shows relationships between the two types of fingerprints (GC/MS and DGGE profiles).Classification methods including Support Vector Machines and Discriminant Partial Least Squaressuggest that mice can be classified according to both background strain and MHC-haplotype. Asexpected, the differences among the mice were much greater between strains that vary at both MHCand background loci than the congenics, which differ only at the MHC. These results indicate that thevolatiles in scent-marks provide information about genetic similarity of the mice, and support the ideathat the production of these genetically determined volatiles is influenced by commensal microflora.This paper describes the application of consensus methods to relate two blocks of analytical data.",

author = "S. Zomer and S.J. Dixon and Y. Xu and S.P. Jensen and H. Wang and C.V. Lanyon and A.G. O'Donnell and A.S. Clare and L.M. Gosling and D.J. Penn and R.G. Brereton",

year = "2009",

doi = "10.1039/b807061j",

language = "English",

volume = "134",

pages = "114--123",

journal = "The Analyst",

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Zomer, S, Dixon, SJ, Xu, Y, Jensen, SP, Wang, H, Lanyon, CV, O'Donnell, AG, Clare, AS, Gosling, LM, Penn, DJ & Brereton, RG 2009, 'Consensus multivariate methods in gas chromatography mass spectrometry and denaturing gradient gel electrophoresis: MHC-congenic and other strains of mice can be classified according to the profiles of volatiles and microflora in their scent-marks', The Analyst, vol. 134, no. 1, pp. 114-123. https://doi.org/10.1039/b807061j

Consensus multivariate methods in gas chromatography mass spectrometry and denaturing gradient gel electrophoresis: MHC-congenic and other strains of mice can be classified according to the profiles of volatiles and microflora in their scent-marks. / Zomer, S.; Dixon, S.J.; Xu, Y. et al.
In: The Analyst, Vol. 134, No. 1, 2009, p. 114-123.

Research output: Contribution to journal › Article › peer-review

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T1 - Consensus multivariate methods in gas chromatography mass spectrometry and denaturing gradient gel electrophoresis: MHC-congenic and other strains of mice can be classified according to the profiles of volatiles and microflora in their scent-marks

AU - Zomer, S.

AU - Dixon, S.J.

AU - Xu, Y.

AU - Jensen, S.P.

AU - Wang, H.

AU - Lanyon, C.V.

AU - O'Donnell, A.G.

AU - Clare, A.S.

AU - Gosling, L.M.

AU - Penn, D.J.

AU - Brereton, R.G.

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AB - House mice (Mus domesticus) communicate using scent-marks, and the chemical and microbialcomposition of these ‘extended phenotypes’ are both influenced by genetics. This study examined howthe genes of the major histocompatibility complex (MHC) and background genes influence the volatilecompounds (analysed with Gas Chromatography Mass Spectrometry or GC/MS) and microbialcommunities (analysed using Denaturating Gradient Gel Electrophoresis or DGGE) in scent-marksproduced by congenic strains of mice. The use of Consensus Principal Components Analysis isdescribed and shows relationships between the two types of fingerprints (GC/MS and DGGE profiles).Classification methods including Support Vector Machines and Discriminant Partial Least Squaressuggest that mice can be classified according to both background strain and MHC-haplotype. Asexpected, the differences among the mice were much greater between strains that vary at both MHCand background loci than the congenics, which differ only at the MHC. These results indicate that thevolatiles in scent-marks provide information about genetic similarity of the mice, and support the ideathat the production of these genetically determined volatiles is influenced by commensal microflora.This paper describes the application of consensus methods to relate two blocks of analytical data.

U2 - 10.1039/b807061j

DO - 10.1039/b807061j

M3 - Article

SN - 0003-2654

VL - 134

SP - 114

EP - 123

JO - The Analyst

JF - The Analyst

IS - 1

ER -