Conscious wireless electroretinogram and visual evoked potentials in rats

Jason Charng, Christine T. Nguyen, Zheng He, Trung M. Dang, Algis J. Vingrys, Rebecca L. Fish, Rachel Gurrell, Phil Brain, Bang V. Bui

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

The electroretinogram (ERG, retina) and visual evoked potential (VEP, brain) are widely used in vivo tools assaying the integrity of the visual pathway. Current recordings in preclinical models are conducted under anesthesia, which alters neural physiology and contaminates responses. We describe a conscious wireless ERG and VEP recording platform in rats. Using a novel surgical technique to chronically implant electrodes subconjunctivally on the eye and epidurally over the visual cortex, we are able to record stable and repeatable conscious ERG and VEP signals over at least 1 month. We show that the use of anaesthetics, necessary for conventional ERG and VEP measurements, alters electrophysiology recordings. Conscious visual electrophysiology improves the viability of longitudinal studies by eliminating complications associated with repeated anaesthesia. It will also enable uncontaminated assessment of drug effects, allowing the eye to be used as an effective biomarker of the central nervous system.

Original languageEnglish
Article numbere74172
Number of pages14
JournalPLoS One
Volume8
Issue number9
DOIs
Publication statusPublished - 12 Sep 2013
Externally publishedYes

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