Comprehensive characteristics and genetic diversity of the endemic Australian Viola banksii (section Erpetion, Violaceae)

Monika Kwiatkowska, Justyna Zabicka, Grzegorz Migdalek, Piotr Zabicki, Marlena Cubala, Jerzy Bohdanowicz, Aneta Slomka, Monika Jedrzejczyk-Korycinska, Elwira Sliwinska, Klaudia Sychta, Thomas Marcussen, Kevin Thiele, Elzbieta Kuta

Research output: Contribution to journalArticle

Abstract

Viola banksii, the type species of section Erpetion, is endemic in eastern mainland Australia. In this paper we characterise morphological and anatomical features and assess genome size and genetic diversity in combination with the breeding system. V. banksii develops exclusively chasmogamous flowers. Ovules are anatropous, crassinucellate and bitegmic, the female gametophyte is of the Polygonum type, and the embryo is of Asterad type surrounded by nuclear endosperm. Pollen is non-heteromorphic, 3-aperturate, and highly viable. V. banksii grows in shade on moist, well drained, often sandy soils, and this is reflected in the anatomy of its organs, which includes a lack of subepidermal collenchyma in aerial parts, large leaf epidermal cells with thin cell walls, a narrow cuticle layer, and vascular bundles with xylem that are not rich in vessels. V. banksii is tolerant to zinc and lead based on phytotoxicity test. The high chromosome number (2n=10x=50) does not correspond to a small genome size (2CDNA=1.27 pg). Lowmean intra-populational gene diversity (H-S = 0.077) detected by ISSR markers confirms the strong influence of selfing and clonal propagation by pseudostolons. Unique morphological traits of V. banksii include nyctinastic petal movement, the lack of afloral spur, the presence of gland-like protuberances on two stamens, and the presence of pseudostolons, which could be a synapomorphy for the whole section.

Original languageEnglish
Pages (from-to)81-98
Number of pages18
JournalAustralian Journal of Botany
Volume67
Issue number2
DOIs
Publication statusPublished - 2019

Cite this

Kwiatkowska, M., Zabicka, J., Migdalek, G., Zabicki, P., Cubala, M., Bohdanowicz, J., ... Kuta, E. (2019). Comprehensive characteristics and genetic diversity of the endemic Australian Viola banksii (section Erpetion, Violaceae). Australian Journal of Botany, 67(2), 81-98. https://doi.org/10.1071/BT18233
Kwiatkowska, Monika ; Zabicka, Justyna ; Migdalek, Grzegorz ; Zabicki, Piotr ; Cubala, Marlena ; Bohdanowicz, Jerzy ; Slomka, Aneta ; Jedrzejczyk-Korycinska, Monika ; Sliwinska, Elwira ; Sychta, Klaudia ; Marcussen, Thomas ; Thiele, Kevin ; Kuta, Elzbieta. / Comprehensive characteristics and genetic diversity of the endemic Australian Viola banksii (section Erpetion, Violaceae). In: Australian Journal of Botany. 2019 ; Vol. 67, No. 2. pp. 81-98.
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abstract = "Viola banksii, the type species of section Erpetion, is endemic in eastern mainland Australia. In this paper we characterise morphological and anatomical features and assess genome size and genetic diversity in combination with the breeding system. V. banksii develops exclusively chasmogamous flowers. Ovules are anatropous, crassinucellate and bitegmic, the female gametophyte is of the Polygonum type, and the embryo is of Asterad type surrounded by nuclear endosperm. Pollen is non-heteromorphic, 3-aperturate, and highly viable. V. banksii grows in shade on moist, well drained, often sandy soils, and this is reflected in the anatomy of its organs, which includes a lack of subepidermal collenchyma in aerial parts, large leaf epidermal cells with thin cell walls, a narrow cuticle layer, and vascular bundles with xylem that are not rich in vessels. V. banksii is tolerant to zinc and lead based on phytotoxicity test. The high chromosome number (2n=10x=50) does not correspond to a small genome size (2CDNA=1.27 pg). Lowmean intra-populational gene diversity (H-S = 0.077) detected by ISSR markers confirms the strong influence of selfing and clonal propagation by pseudostolons. Unique morphological traits of V. banksii include nyctinastic petal movement, the lack of afloral spur, the presence of gland-like protuberances on two stamens, and the presence of pseudostolons, which could be a synapomorphy for the whole section.",
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author = "Monika Kwiatkowska and Justyna Zabicka and Grzegorz Migdalek and Piotr Zabicki and Marlena Cubala and Jerzy Bohdanowicz and Aneta Slomka and Monika Jedrzejczyk-Korycinska and Elwira Sliwinska and Klaudia Sychta and Thomas Marcussen and Kevin Thiele and Elzbieta Kuta",
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Kwiatkowska, M, Zabicka, J, Migdalek, G, Zabicki, P, Cubala, M, Bohdanowicz, J, Slomka, A, Jedrzejczyk-Korycinska, M, Sliwinska, E, Sychta, K, Marcussen, T, Thiele, K & Kuta, E 2019, 'Comprehensive characteristics and genetic diversity of the endemic Australian Viola banksii (section Erpetion, Violaceae)' Australian Journal of Botany, vol. 67, no. 2, pp. 81-98. https://doi.org/10.1071/BT18233

Comprehensive characteristics and genetic diversity of the endemic Australian Viola banksii (section Erpetion, Violaceae). / Kwiatkowska, Monika; Zabicka, Justyna; Migdalek, Grzegorz; Zabicki, Piotr; Cubala, Marlena; Bohdanowicz, Jerzy; Slomka, Aneta; Jedrzejczyk-Korycinska, Monika; Sliwinska, Elwira; Sychta, Klaudia; Marcussen, Thomas; Thiele, Kevin; Kuta, Elzbieta.

In: Australian Journal of Botany, Vol. 67, No. 2, 2019, p. 81-98.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Comprehensive characteristics and genetic diversity of the endemic Australian Viola banksii (section Erpetion, Violaceae)

AU - Kwiatkowska, Monika

AU - Zabicka, Justyna

AU - Migdalek, Grzegorz

AU - Zabicki, Piotr

AU - Cubala, Marlena

AU - Bohdanowicz, Jerzy

AU - Slomka, Aneta

AU - Jedrzejczyk-Korycinska, Monika

AU - Sliwinska, Elwira

AU - Sychta, Klaudia

AU - Marcussen, Thomas

AU - Thiele, Kevin

AU - Kuta, Elzbieta

PY - 2019

Y1 - 2019

N2 - Viola banksii, the type species of section Erpetion, is endemic in eastern mainland Australia. In this paper we characterise morphological and anatomical features and assess genome size and genetic diversity in combination with the breeding system. V. banksii develops exclusively chasmogamous flowers. Ovules are anatropous, crassinucellate and bitegmic, the female gametophyte is of the Polygonum type, and the embryo is of Asterad type surrounded by nuclear endosperm. Pollen is non-heteromorphic, 3-aperturate, and highly viable. V. banksii grows in shade on moist, well drained, often sandy soils, and this is reflected in the anatomy of its organs, which includes a lack of subepidermal collenchyma in aerial parts, large leaf epidermal cells with thin cell walls, a narrow cuticle layer, and vascular bundles with xylem that are not rich in vessels. V. banksii is tolerant to zinc and lead based on phytotoxicity test. The high chromosome number (2n=10x=50) does not correspond to a small genome size (2CDNA=1.27 pg). Lowmean intra-populational gene diversity (H-S = 0.077) detected by ISSR markers confirms the strong influence of selfing and clonal propagation by pseudostolons. Unique morphological traits of V. banksii include nyctinastic petal movement, the lack of afloral spur, the presence of gland-like protuberances on two stamens, and the presence of pseudostolons, which could be a synapomorphy for the whole section.

AB - Viola banksii, the type species of section Erpetion, is endemic in eastern mainland Australia. In this paper we characterise morphological and anatomical features and assess genome size and genetic diversity in combination with the breeding system. V. banksii develops exclusively chasmogamous flowers. Ovules are anatropous, crassinucellate and bitegmic, the female gametophyte is of the Polygonum type, and the embryo is of Asterad type surrounded by nuclear endosperm. Pollen is non-heteromorphic, 3-aperturate, and highly viable. V. banksii grows in shade on moist, well drained, often sandy soils, and this is reflected in the anatomy of its organs, which includes a lack of subepidermal collenchyma in aerial parts, large leaf epidermal cells with thin cell walls, a narrow cuticle layer, and vascular bundles with xylem that are not rich in vessels. V. banksii is tolerant to zinc and lead based on phytotoxicity test. The high chromosome number (2n=10x=50) does not correspond to a small genome size (2CDNA=1.27 pg). Lowmean intra-populational gene diversity (H-S = 0.077) detected by ISSR markers confirms the strong influence of selfing and clonal propagation by pseudostolons. Unique morphological traits of V. banksii include nyctinastic petal movement, the lack of afloral spur, the presence of gland-like protuberances on two stamens, and the presence of pseudostolons, which could be a synapomorphy for the whole section.

KW - anatomy

KW - flower

KW - genetic diversity

KW - ISSR markers

KW - morphology

KW - tolerance to heavy metals

KW - vegetative organs

KW - Viola banksii

KW - POLLEN HETEROMORPHISM

KW - ULIGINOSA VIOLACEAE

KW - MELANIUM GING.

KW - EVOLUTION

KW - L.

KW - SERPENTINE

KW - MORPHOLOGY

KW - INFERENCE

KW - MARKERS

KW - NUMBER

U2 - 10.1071/BT18233

DO - 10.1071/BT18233

M3 - Article

VL - 67

SP - 81

EP - 98

JO - Australian Journal of Botany

JF - Australian Journal of Botany

SN - 0067-1924

IS - 2

ER -