Complete chloroplast genome sequences of four meliaceae species and comparative analyses

Malte Mader, Birte Pakull, Céline Blanc-Jolivet, Maike Paulini-Drewes, Zoéwindé Henri Noël Bouda, Bernd Degen, Ian Small, Birgit Kersten

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The Meliaceae family mainly consists of trees and shrubs with a pantropical distribution. In this study, the complete chloroplast genomes of four Meliaceae species were sequenced and compared with each other and with the previously published Azadirachta indica plastome. The five plastomes are circular and exhibit a quadripartite structure with high conservation of gene content and order. They include 130 genes encoding 85 proteins, 37 tRNAs and 8 rRNAs. Inverted repeat expansion resulted in a duplication of rps19 in the five Meliaceae species, which is consistent with that in many other Sapindales, but different from many other rosids. Compared to Azadirachta indica, the four newly sequenced Meliaceae individuals share several large deletions, which mainly contribute to the decreased genome sizes. A whole-plastome phylogeny supports previous findings that the four species form a monophyletic sister clade to Azadirachta indica within the Meliaceae. SNPs and indels identified in all complete Meliaceae plastomes might be suitable targets for the future development of genetic markers at different taxonomic levels. The extended analysis of SNPs in the matK gene led to the identification of four potential Meliaceae-specific SNPs as a basis for future validation and marker development.

Original languageEnglish
Article number19
JournalInternational Journal of Molecular Sciences
Volume19
Issue number3
DOIs
Publication statusPublished - 1 Mar 2018

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Meliaceae
Chloroplast Genome
chloroplasts
genome
genes
Genes
markers
Azadirachta
deletion
Single Nucleotide Polymorphism
Gene encoding
conservation
coding
Transfer RNA
proteins
Conservation
expansion
Genome Size
Gene Order
Proteins

Cite this

Mader, M., Pakull, B., Blanc-Jolivet, C., Paulini-Drewes, M., Bouda, Z. H. N., Degen, B., ... Kersten, B. (2018). Complete chloroplast genome sequences of four meliaceae species and comparative analyses. International Journal of Molecular Sciences, 19(3), [19]. https://doi.org/10.3390/ijms19030701
Mader, Malte ; Pakull, Birte ; Blanc-Jolivet, Céline ; Paulini-Drewes, Maike ; Bouda, Zoéwindé Henri Noël ; Degen, Bernd ; Small, Ian ; Kersten, Birgit. / Complete chloroplast genome sequences of four meliaceae species and comparative analyses. In: International Journal of Molecular Sciences. 2018 ; Vol. 19, No. 3.
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Mader, M, Pakull, B, Blanc-Jolivet, C, Paulini-Drewes, M, Bouda, ZHN, Degen, B, Small, I & Kersten, B 2018, 'Complete chloroplast genome sequences of four meliaceae species and comparative analyses' International Journal of Molecular Sciences, vol. 19, no. 3, 19. https://doi.org/10.3390/ijms19030701

Complete chloroplast genome sequences of four meliaceae species and comparative analyses. / Mader, Malte; Pakull, Birte; Blanc-Jolivet, Céline; Paulini-Drewes, Maike; Bouda, Zoéwindé Henri Noël; Degen, Bernd; Small, Ian; Kersten, Birgit.

In: International Journal of Molecular Sciences, Vol. 19, No. 3, 19, 01.03.2018.

Research output: Contribution to journalArticle

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