Complementary Approaches to Imaging Subcellular Lipid Architectures in Live Bacteria Using Phosphorescent Iridium Complexes and Raman Spectroscopy

Anna Maria Ranieri, Chiara Caporale, Valentina Fiorini, Alysia Hubbard, Paul Rigby, Stefano Stagni, Elizabeth Watkin, Mark I. Ogden, Mark J. Hackett, Massimiliano Massi

Research output: Contribution to journalLetter

Abstract

A family of three neutral iridium(III) tetrazolato complexes are investigated as bacterial imaging agents. The complexes offer a facile tuning of the emission colour from green (520 nm) to red (600 nm) in aqueous media, while keeping the excitation wavelength unchanged. The three complexes do not inhibit the bacterial growth of Bacillus Cereus, used as a model in this study, and exhibit extremely fast cellular uptake. After a minute incubation time, the nontoxic complexes show subcellular localisation in spherical structures identified as lipid vacuoles. Confocal Raman imaging has been exploited for the first time on live bacteria, to provide direct and label-free mapping of the lipid-enriched organelles within B. cereus, complementing the use of luminescent probes. Examination of the Raman spectra not only confirmed the presence of lipophilic inclusions in B. cereus but offered additional information about their chemical composition, suggesting that the lipid vacuoles may contain polyhydroxybutyrate (PHB).

Original languageEnglish
Number of pages6
JournalCHEMISTRY-A EUROPEAN JOURNAL
DOIs
Publication statusE-pub ahead of print - Jul 2019

Cite this

Ranieri, Anna Maria ; Caporale, Chiara ; Fiorini, Valentina ; Hubbard, Alysia ; Rigby, Paul ; Stagni, Stefano ; Watkin, Elizabeth ; Ogden, Mark I. ; Hackett, Mark J. ; Massi, Massimiliano. / Complementary Approaches to Imaging Subcellular Lipid Architectures in Live Bacteria Using Phosphorescent Iridium Complexes and Raman Spectroscopy. In: CHEMISTRY-A EUROPEAN JOURNAL. 2019.
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abstract = "A family of three neutral iridium(III) tetrazolato complexes are investigated as bacterial imaging agents. The complexes offer a facile tuning of the emission colour from green (520 nm) to red (600 nm) in aqueous media, while keeping the excitation wavelength unchanged. The three complexes do not inhibit the bacterial growth of Bacillus Cereus, used as a model in this study, and exhibit extremely fast cellular uptake. After a minute incubation time, the nontoxic complexes show subcellular localisation in spherical structures identified as lipid vacuoles. Confocal Raman imaging has been exploited for the first time on live bacteria, to provide direct and label-free mapping of the lipid-enriched organelles within B. cereus, complementing the use of luminescent probes. Examination of the Raman spectra not only confirmed the presence of lipophilic inclusions in B. cereus but offered additional information about their chemical composition, suggesting that the lipid vacuoles may contain polyhydroxybutyrate (PHB).",
keywords = "bacterial imaging, imaging agents, iridium, luminescence, Raman spectroscopy, PROBES, METAL, LOCALIZATION, MICROSCOPY, PROSPECTS, PROGRESS",
author = "Ranieri, {Anna Maria} and Chiara Caporale and Valentina Fiorini and Alysia Hubbard and Paul Rigby and Stefano Stagni and Elizabeth Watkin and Ogden, {Mark I.} and Hackett, {Mark J.} and Massimiliano Massi",
year = "2019",
month = "7",
doi = "10.1002/chem.201902023",
language = "English",
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Complementary Approaches to Imaging Subcellular Lipid Architectures in Live Bacteria Using Phosphorescent Iridium Complexes and Raman Spectroscopy. / Ranieri, Anna Maria; Caporale, Chiara; Fiorini, Valentina; Hubbard, Alysia; Rigby, Paul; Stagni, Stefano; Watkin, Elizabeth; Ogden, Mark I.; Hackett, Mark J.; Massi, Massimiliano.

In: CHEMISTRY-A EUROPEAN JOURNAL, 07.2019.

Research output: Contribution to journalLetter

TY - JOUR

T1 - Complementary Approaches to Imaging Subcellular Lipid Architectures in Live Bacteria Using Phosphorescent Iridium Complexes and Raman Spectroscopy

AU - Ranieri, Anna Maria

AU - Caporale, Chiara

AU - Fiorini, Valentina

AU - Hubbard, Alysia

AU - Rigby, Paul

AU - Stagni, Stefano

AU - Watkin, Elizabeth

AU - Ogden, Mark I.

AU - Hackett, Mark J.

AU - Massi, Massimiliano

PY - 2019/7

Y1 - 2019/7

N2 - A family of three neutral iridium(III) tetrazolato complexes are investigated as bacterial imaging agents. The complexes offer a facile tuning of the emission colour from green (520 nm) to red (600 nm) in aqueous media, while keeping the excitation wavelength unchanged. The three complexes do not inhibit the bacterial growth of Bacillus Cereus, used as a model in this study, and exhibit extremely fast cellular uptake. After a minute incubation time, the nontoxic complexes show subcellular localisation in spherical structures identified as lipid vacuoles. Confocal Raman imaging has been exploited for the first time on live bacteria, to provide direct and label-free mapping of the lipid-enriched organelles within B. cereus, complementing the use of luminescent probes. Examination of the Raman spectra not only confirmed the presence of lipophilic inclusions in B. cereus but offered additional information about their chemical composition, suggesting that the lipid vacuoles may contain polyhydroxybutyrate (PHB).

AB - A family of three neutral iridium(III) tetrazolato complexes are investigated as bacterial imaging agents. The complexes offer a facile tuning of the emission colour from green (520 nm) to red (600 nm) in aqueous media, while keeping the excitation wavelength unchanged. The three complexes do not inhibit the bacterial growth of Bacillus Cereus, used as a model in this study, and exhibit extremely fast cellular uptake. After a minute incubation time, the nontoxic complexes show subcellular localisation in spherical structures identified as lipid vacuoles. Confocal Raman imaging has been exploited for the first time on live bacteria, to provide direct and label-free mapping of the lipid-enriched organelles within B. cereus, complementing the use of luminescent probes. Examination of the Raman spectra not only confirmed the presence of lipophilic inclusions in B. cereus but offered additional information about their chemical composition, suggesting that the lipid vacuoles may contain polyhydroxybutyrate (PHB).

KW - bacterial imaging

KW - imaging agents

KW - iridium

KW - luminescence

KW - Raman spectroscopy

KW - PROBES

KW - METAL

KW - LOCALIZATION

KW - MICROSCOPY

KW - PROSPECTS

KW - PROGRESS

U2 - 10.1002/chem.201902023

DO - 10.1002/chem.201902023

M3 - Letter

JO - CHEMISTRY-A EUROPEAN JOURNAL

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