Comparitive proteomic analysis of nonmalignant and early stage prostate cancers

Research output: ThesisDoctoral Thesis

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Abstract

[Truncated] Prostate cancer is the most common cause of non-cutaneous malignancy in men and although frequently latent, is the second commonest cause of cancer deaths. The aetiology of prostate cancer is largely undefined as are protein and genetic alterations characteristic of early stage prostate cancers. Using proteomics, this study mapped and compared the protein profiles of nonmalignant and malignant prostate tissues with the primary aim of identifying proteins that may be involved in the development of early stage prostate cancers. In total, 57 proteins with reduced levels in malignant compared to nonmalignant tissues were selected and 23 were conclusively identified using mass spectrometry and protein sequencing. In addition, 69 proteins with increased levels in malignant tissues were selected. Of the 23 proteins identified, 7 were examined further using one- and two- dimensional western blotting and immunohistochemistry to characterise protein forms present within tissues and cellular distribution of individual proteins.
Original languageEnglish
QualificationDoctor of Philosophy
Awarding Institution
  • The University of Western Australia
DOIs
Publication statusUnpublished - 2002

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