Comparison of Molecular and Conventional Methods for Typing of Enteroviral Isolates

D.J. Manayani; R.V. Shaji; G.J. Fletcher; T. Cherian; Murali Murali; N. Sathish; T. Solomon; C. Cnanamuthu; G. Sridharan

doi:10.1128/JCM.40.3.1069-1070.2002

Comparison of Molecular and Conventional Methods for Typing of Enteroviral Isolates

D.J. Manayani, R.V. Shaji, G.J. Fletcher, T. Cherian, Murali Murali, N. Sathish, T. Solomon, C. Cnanamuthu, G. Sridharan

Research output: Contribution to journal › Article › peer-review

10 Citations (Scopus)

Abstract

Twenty-eight enteroviral isolates obtained from various clinical specimens were typed by Lim-Benyesh-Melnick (LBM) pool-based neutralization, PCR-restriction fragment length polymorphism (RFLP), and partial sequencing of the VP1 region of the enteroviral genome. Sequencing was found to be a good alternative to LBM typing, while PCR-RFLP was inadequate for identification of enteroviral isolates.

Original language	English
Pages (from-to)	1069-1070
Journal	Journal of Clinical Microbiology
Volume	40
Issue number	3
DOIs	https://doi.org/10.1128/JCM.40.3.1069-1070.2002
Publication status	Published - 2002

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abstract = "Twenty-eight enteroviral isolates obtained from various clinical specimens were typed by Lim-Benyesh-Melnick (LBM) pool-based neutralization, PCR-restriction fragment length polymorphism (RFLP), and partial sequencing of the VP1 region of the enteroviral genome. Sequencing was found to be a good alternative to LBM typing, while PCR-RFLP was inadequate for identification of enteroviral isolates.",

author = "D.J. Manayani and R.V. Shaji and G.J. Fletcher and T. Cherian and Murali Murali and N. Sathish and T. Solomon and C. Cnanamuthu and G. Sridharan",

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AU - Manayani, D.J.

AU - Shaji, R.V.

AU - Fletcher, G.J.

AU - Cherian, T.

AU - Murali, Murali

AU - Sathish, N.

AU - Solomon, T.

AU - Cnanamuthu, C.

AU - Sridharan, G.

PY - 2002

Y1 - 2002

N2 - Twenty-eight enteroviral isolates obtained from various clinical specimens were typed by Lim-Benyesh-Melnick (LBM) pool-based neutralization, PCR-restriction fragment length polymorphism (RFLP), and partial sequencing of the VP1 region of the enteroviral genome. Sequencing was found to be a good alternative to LBM typing, while PCR-RFLP was inadequate for identification of enteroviral isolates.

AB - Twenty-eight enteroviral isolates obtained from various clinical specimens were typed by Lim-Benyesh-Melnick (LBM) pool-based neutralization, PCR-restriction fragment length polymorphism (RFLP), and partial sequencing of the VP1 region of the enteroviral genome. Sequencing was found to be a good alternative to LBM typing, while PCR-RFLP was inadequate for identification of enteroviral isolates.

UR - https://www.scopus.com/pages/publications/0036194676

U2 - 10.1128/JCM.40.3.1069-1070.2002

DO - 10.1128/JCM.40.3.1069-1070.2002

M3 - Article

SN - 0095-1137

VL - 40

SP - 1069

EP - 1070

JO - Journal of Clinical Microbiology

JF - Journal of Clinical Microbiology

IS - 3

ER -

Comparison of Molecular and Conventional Methods for Typing of Enteroviral Isolates

Abstract

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