Comparison of Molecular and Conventional Methods for Typing of Enteroviral Isolates

D.J. Manayani; R.V. Shaji; G.J. Fletcher; T. Cherian; Murali Murali; N. Sathish; T. Solomon; C. Cnanamuthu; G. Sridharan

doi:10.1128/JCM.40.3.1069-1070.2002

Comparison of Molecular and Conventional Methods for Typing of Enteroviral Isolates

D.J. Manayani, R.V. Shaji, G.J. Fletcher, T. Cherian, Murali Murali, N. Sathish, T. Solomon, C. Cnanamuthu, G. Sridharan

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10 Citations (Scopus)

Abstract

Twenty-eight enteroviral isolates obtained from various clinical specimens were typed by Lim-Benyesh-Melnick (LBM) pool-based neutralization, PCR-restriction fragment length polymorphism (RFLP), and partial sequencing of the VP1 region of the enteroviral genome. Sequencing was found to be a good alternative to LBM typing, while PCR-RFLP was inadequate for identification of enteroviral isolates.

Original language	English
Pages (from-to)	1069-1070
Journal	Journal of Clinical Microbiology
Volume	40
Issue number	3
DOIs	https://doi.org/10.1128/JCM.40.3.1069-1070.2002
Publication status	Published - 2002

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title = "Comparison of Molecular and Conventional Methods for Typing of Enteroviral Isolates",

abstract = "Twenty-eight enteroviral isolates obtained from various clinical specimens were typed by Lim-Benyesh-Melnick (LBM) pool-based neutralization, PCR-restriction fragment length polymorphism (RFLP), and partial sequencing of the VP1 region of the enteroviral genome. Sequencing was found to be a good alternative to LBM typing, while PCR-RFLP was inadequate for identification of enteroviral isolates.",

author = "D.J. Manayani and R.V. Shaji and G.J. Fletcher and T. Cherian and Murali Murali and N. Sathish and T. Solomon and C. Cnanamuthu and G. Sridharan",

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AU - Manayani, D.J.

AU - Shaji, R.V.

AU - Fletcher, G.J.

AU - Cherian, T.

AU - Murali, Murali

AU - Sathish, N.

AU - Solomon, T.

AU - Cnanamuthu, C.

AU - Sridharan, G.

PY - 2002

Y1 - 2002

N2 - Twenty-eight enteroviral isolates obtained from various clinical specimens were typed by Lim-Benyesh-Melnick (LBM) pool-based neutralization, PCR-restriction fragment length polymorphism (RFLP), and partial sequencing of the VP1 region of the enteroviral genome. Sequencing was found to be a good alternative to LBM typing, while PCR-RFLP was inadequate for identification of enteroviral isolates.

AB - Twenty-eight enteroviral isolates obtained from various clinical specimens were typed by Lim-Benyesh-Melnick (LBM) pool-based neutralization, PCR-restriction fragment length polymorphism (RFLP), and partial sequencing of the VP1 region of the enteroviral genome. Sequencing was found to be a good alternative to LBM typing, while PCR-RFLP was inadequate for identification of enteroviral isolates.

U2 - 10.1128/JCM.40.3.1069-1070.2002

DO - 10.1128/JCM.40.3.1069-1070.2002

M3 - Article

VL - 40

SP - 1069

EP - 1070

JO - Journal of Clinical Microbiology

JF - Journal of Clinical Microbiology

SN - 0095-1137

IS - 3

ER -

Comparison of Molecular and Conventional Methods for Typing of Enteroviral Isolates

Abstract

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