TY - JOUR
T1 - Comparison of kinin B1 and B2 receptor expression in neutrophils of asthmatic and non-asthmatic subjects
AU - Bertram, C.
AU - Misso, Neil
AU - Fogel-Petrovic, Mirjana
AU - Figueroa, C.
AU - Thompson, Philip
AU - Bhoola, Kanti
PY - 2007
Y1 - 2007
N2 - Kinins have been implicated in the pathophysiology of asthma and activation of kinin receptors stimulates neutrophil chemotaxis. However, the expression of kinin receptors on neutrophils of asthmatic subjects has not been assessed. The aim of this study was to compare the expression of kinin B-1 and B-2 receptor mRNA and proteins in neutrophils of asthmatic and non-asthmatic subjects, and to assess whether inhaled corticosteroid treatment may influence expression of the kinin receptors. Neutrophils were isolated from peripheral blood of asthmatic (n = 27) and non-asthmatic subjects (n = 14). The presence of kinin B-1 and B-2 receptor protein on neutrophils was confirmed by immunolabeling with specific antibodies followed by immunoperoxidase, immunofluorescence and FACS detection. Kinin B-1 and B-2 receptor mRNA expression was assessed by RT-PCR. Quantitative image analysis of fluorescence immunolabeled neutrophils showed no differences in kinin B-1 or B-2 receptor protein expression between asthmatic and non-asthmatic subjects. Similarly, quantitative real time RT-PCR analysis demonstrated no differences in expression of mRNA for the kinin B-1 or B-2 receptors between asthmatic and non-asthmatic subjects. However, B-1 receptor mRNA expression was significantly lower in asthmatic subjects using >= 2000 mu g of inhaled corticosteroid per day (p < 0.05) and B-1 receptor protein levels also tended to be lower in these subjects. Corticosteroids may have a beneficial anti-inflammatory effect in asthma by down-regulating B, receptor expression on neutrophils, thereby decreasing the migration of these inflammatory cells into the airways. (c) 2007 Elsevier B.V. All rights reserved.
AB - Kinins have been implicated in the pathophysiology of asthma and activation of kinin receptors stimulates neutrophil chemotaxis. However, the expression of kinin receptors on neutrophils of asthmatic subjects has not been assessed. The aim of this study was to compare the expression of kinin B-1 and B-2 receptor mRNA and proteins in neutrophils of asthmatic and non-asthmatic subjects, and to assess whether inhaled corticosteroid treatment may influence expression of the kinin receptors. Neutrophils were isolated from peripheral blood of asthmatic (n = 27) and non-asthmatic subjects (n = 14). The presence of kinin B-1 and B-2 receptor protein on neutrophils was confirmed by immunolabeling with specific antibodies followed by immunoperoxidase, immunofluorescence and FACS detection. Kinin B-1 and B-2 receptor mRNA expression was assessed by RT-PCR. Quantitative image analysis of fluorescence immunolabeled neutrophils showed no differences in kinin B-1 or B-2 receptor protein expression between asthmatic and non-asthmatic subjects. Similarly, quantitative real time RT-PCR analysis demonstrated no differences in expression of mRNA for the kinin B-1 or B-2 receptors between asthmatic and non-asthmatic subjects. However, B-1 receptor mRNA expression was significantly lower in asthmatic subjects using >= 2000 mu g of inhaled corticosteroid per day (p < 0.05) and B-1 receptor protein levels also tended to be lower in these subjects. Corticosteroids may have a beneficial anti-inflammatory effect in asthma by down-regulating B, receptor expression on neutrophils, thereby decreasing the migration of these inflammatory cells into the airways. (c) 2007 Elsevier B.V. All rights reserved.
U2 - 10.1016/j.intimp.2007.07.012
DO - 10.1016/j.intimp.2007.07.012
M3 - Article
C2 - 18039523
VL - 7
SP - 1862
EP - 1868
JO - International Immunopharmacology
JF - International Immunopharmacology
SN - 1567-5769
IS - 14
ER -