TY - JOUR
T1 - Comparison of growth rates of bovine retinal and brain microvascular pericytes in different oxygen concentrations in vitro
AU - Wong, H.C.
AU - Thompson, S.
AU - Yu, Dao-Yi
AU - Cringle, Stephen
AU - Alder, V.A.
AU - Taylor, S.J.
PY - 1995
Y1 - 1995
N2 - Background: The hyperoxic injury of the microcirculation in the central nervous system appears to be specific to the retina in premature mammals. Oxygen tensions in normal adult mammalian retina and brain vary between nearly 0 and 90 mmHg. This study sought to compare the in vitro replication of retinal and brain microvascular pericytes in normal glucose medium and in 1%, 5% and 20% oxygen (equivalent to 15 mmHg, 35 mmHg and 150 mmHg, respectively).Methods: A preliminary study, using oxygen microelectrodes, confirmed that the pericellular oxygen tension of pericytes, cultured in medium under air, was within 13 mmHg of the tension of the gas phase above the media.Pericytes were highly enriched by magnetic antibody cell sorting with the anti-pericyte monoclonal antibody (3G5) to 95% to 99% purity, to remove cell contaminants which may have invalidated the mitogenic assay.Results: Mitogenic assays showed that brain pericytes replicated faster than their counterparts from retina (P
AB - Background: The hyperoxic injury of the microcirculation in the central nervous system appears to be specific to the retina in premature mammals. Oxygen tensions in normal adult mammalian retina and brain vary between nearly 0 and 90 mmHg. This study sought to compare the in vitro replication of retinal and brain microvascular pericytes in normal glucose medium and in 1%, 5% and 20% oxygen (equivalent to 15 mmHg, 35 mmHg and 150 mmHg, respectively).Methods: A preliminary study, using oxygen microelectrodes, confirmed that the pericellular oxygen tension of pericytes, cultured in medium under air, was within 13 mmHg of the tension of the gas phase above the media.Pericytes were highly enriched by magnetic antibody cell sorting with the anti-pericyte monoclonal antibody (3G5) to 95% to 99% purity, to remove cell contaminants which may have invalidated the mitogenic assay.Results: Mitogenic assays showed that brain pericytes replicated faster than their counterparts from retina (P
U2 - 10.1111/j.1442-9071.1995.tb00180.x
DO - 10.1111/j.1442-9071.1995.tb00180.x
M3 - Article
VL - 23
SP - 299
EP - 308
JO - Australian and New Zealand Journal of Ophthalmology
JF - Australian and New Zealand Journal of Ophthalmology
IS - not known
ER -