Comparison of gravimetric, creamatocrit and esterified fatty acid methods for determination of total fat content in human milk

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6 Citations (Scopus)

Abstract

The gravimetric method is considered the gold standard for measuring the fat content of human milk. However, it is labor intensive and requires large volumes of human milk. Other methods, such as creamatocrit and esterified fatty acid assay (EFA), have also been used widely in fat analysis. However, these methods have not been compared concurrently with the gravimetric method. Comparison of the three methods was conducted with human milk of varying fat content. Correlations between these methods were high (r2 = 0.99). Statistical differences (P <0.001) were observed in the overall fat measurements and within each group (low, medium and high fat milk) using the three methods. Overall, stronger correlation with lower mean (4.73 g/L) and percentage differences (5.16%) was observed with the creamatocrit than the EFA method when compared to the gravimetric method. Furthermore, the ease of operation and real-time analysis make the creamatocrit method preferable.

Original languageEnglish
Pages (from-to)505-510
Number of pages6
JournalFood Chemistry
Volume217
DOIs
Publication statusPublished - 15 Feb 2017

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Human Milk
breast milk
Fatty Acids
Fats
lipid content
fatty acids
Assays
methodology
human milk fat
Personnel
assays
lipids
gold
milk fat
labor
Milk

Cite this

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title = "Comparison of gravimetric, creamatocrit and esterified fatty acid methods for determination of total fat content in human milk",
abstract = "The gravimetric method is considered the gold standard for measuring the fat content of human milk. However, it is labor intensive and requires large volumes of human milk. Other methods, such as creamatocrit and esterified fatty acid assay (EFA), have also been used widely in fat analysis. However, these methods have not been compared concurrently with the gravimetric method. Comparison of the three methods was conducted with human milk of varying fat content. Correlations between these methods were high (r2 = 0.99). Statistical differences (P <0.001) were observed in the overall fat measurements and within each group (low, medium and high fat milk) using the three methods. Overall, stronger correlation with lower mean (4.73 g/L) and percentage differences (5.16{\%}) was observed with the creamatocrit than the EFA method when compared to the gravimetric method. Furthermore, the ease of operation and real-time analysis make the creamatocrit method preferable.",
keywords = "Creamatocrit, Esterified fatty acids, Fat content, Gravimetric, Human milk, Lactation",
author = "Jian Du and Gay, {Melvin C L} and Lai, {Ching Tat} and Trengove, {Robert D.} and Hartmann, {Peter E.} and Geddes, {Donna T.}",
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T1 - Comparison of gravimetric, creamatocrit and esterified fatty acid methods for determination of total fat content in human milk

AU - Du, Jian

AU - Gay, Melvin C L

AU - Lai, Ching Tat

AU - Trengove, Robert D.

AU - Hartmann, Peter E.

AU - Geddes, Donna T.

PY - 2017/2/15

Y1 - 2017/2/15

N2 - The gravimetric method is considered the gold standard for measuring the fat content of human milk. However, it is labor intensive and requires large volumes of human milk. Other methods, such as creamatocrit and esterified fatty acid assay (EFA), have also been used widely in fat analysis. However, these methods have not been compared concurrently with the gravimetric method. Comparison of the three methods was conducted with human milk of varying fat content. Correlations between these methods were high (r2 = 0.99). Statistical differences (P <0.001) were observed in the overall fat measurements and within each group (low, medium and high fat milk) using the three methods. Overall, stronger correlation with lower mean (4.73 g/L) and percentage differences (5.16%) was observed with the creamatocrit than the EFA method when compared to the gravimetric method. Furthermore, the ease of operation and real-time analysis make the creamatocrit method preferable.

AB - The gravimetric method is considered the gold standard for measuring the fat content of human milk. However, it is labor intensive and requires large volumes of human milk. Other methods, such as creamatocrit and esterified fatty acid assay (EFA), have also been used widely in fat analysis. However, these methods have not been compared concurrently with the gravimetric method. Comparison of the three methods was conducted with human milk of varying fat content. Correlations between these methods were high (r2 = 0.99). Statistical differences (P <0.001) were observed in the overall fat measurements and within each group (low, medium and high fat milk) using the three methods. Overall, stronger correlation with lower mean (4.73 g/L) and percentage differences (5.16%) was observed with the creamatocrit than the EFA method when compared to the gravimetric method. Furthermore, the ease of operation and real-time analysis make the creamatocrit method preferable.

KW - Creamatocrit

KW - Esterified fatty acids

KW - Fat content

KW - Gravimetric

KW - Human milk

KW - Lactation

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