Comparison of bioassay and high performance liquid chromatographic assay of artesunate and dihydroartemisinin in plasma

B.M. Kotecka; K.H. Rieckmann; Timothy Davis; K.T. Batty; Kenneth Ilett

doi:10.1016/S0001-706X(03)00118-9

Comparison of bioassay and high performance liquid chromatographic assay of artesunate and dihydroartemisinin in plasma

B.M. Kotecka, K.H. Rieckmann, Timothy Davis, K.T. Batty, Kenneth Ilett

Research output: Contribution to journal › Article › peer-review

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Abstract

The study was a comparison of bioassay and HPLC analysis of artesunate (ARTS) and dihydroartemisinin (DHA) in plasma. ARTS and DHA in plasma samples from patients treated with ARTS were quantified by HPLC and expressed as DHA. DHA-equivalents in the same plasma samples were measured using a standardised parasite culture technique. DHA concentrations estimated by both methods were highly correlated (bioassay = 0.96 x HPLC + 11.0; r(2) = 0.92). At high concentrations ( > 12 000 nmol/l) bioassay sometimes overestimated DHA. Bioassay of active drug in plasma correlates well with specific chemical analysis by HPLC. ARTS and DHA appear to account for the total antimalarial activity in plasma after ARTS administration. (C) 2003 Elsevier Science B.V. All rights reserved.

Original language	English
Pages (from-to)	371-375
Journal	Acta Tropica
Volume	87
DOIs	https://doi.org/10.1016/S0001-706X(03)00118-9
Publication status	Published - 2003

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title = "Comparison of bioassay and high performance liquid chromatographic assay of artesunate and dihydroartemisinin in plasma",

abstract = "The study was a comparison of bioassay and HPLC analysis of artesunate (ARTS) and dihydroartemisinin (DHA) in plasma. ARTS and DHA in plasma samples from patients treated with ARTS were quantified by HPLC and expressed as DHA. DHA-equivalents in the same plasma samples were measured using a standardised parasite culture technique. DHA concentrations estimated by both methods were highly correlated (bioassay = 0.96 x HPLC + 11.0; r(2) = 0.92). At high concentrations ( > 12 000 nmol/l) bioassay sometimes overestimated DHA. Bioassay of active drug in plasma correlates well with specific chemical analysis by HPLC. ARTS and DHA appear to account for the total antimalarial activity in plasma after ARTS administration. (C) 2003 Elsevier Science B.V. All rights reserved.",

author = "B.M. Kotecka and K.H. Rieckmann and Timothy Davis and K.T. Batty and Kenneth Ilett",

year = "2003",

doi = "10.1016/S0001-706X(03)00118-9",

language = "English",

volume = "87",

pages = "371--375",

journal = "Acta Tropica",

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TY - JOUR

T1 - Comparison of bioassay and high performance liquid chromatographic assay of artesunate and dihydroartemisinin in plasma

AU - Kotecka, B.M.

AU - Rieckmann, K.H.

AU - Davis, Timothy

AU - Batty, K.T.

AU - Ilett, Kenneth

PY - 2003

Y1 - 2003

N2 - The study was a comparison of bioassay and HPLC analysis of artesunate (ARTS) and dihydroartemisinin (DHA) in plasma. ARTS and DHA in plasma samples from patients treated with ARTS were quantified by HPLC and expressed as DHA. DHA-equivalents in the same plasma samples were measured using a standardised parasite culture technique. DHA concentrations estimated by both methods were highly correlated (bioassay = 0.96 x HPLC + 11.0; r(2) = 0.92). At high concentrations ( > 12 000 nmol/l) bioassay sometimes overestimated DHA. Bioassay of active drug in plasma correlates well with specific chemical analysis by HPLC. ARTS and DHA appear to account for the total antimalarial activity in plasma after ARTS administration. (C) 2003 Elsevier Science B.V. All rights reserved.

AB - The study was a comparison of bioassay and HPLC analysis of artesunate (ARTS) and dihydroartemisinin (DHA) in plasma. ARTS and DHA in plasma samples from patients treated with ARTS were quantified by HPLC and expressed as DHA. DHA-equivalents in the same plasma samples were measured using a standardised parasite culture technique. DHA concentrations estimated by both methods were highly correlated (bioassay = 0.96 x HPLC + 11.0; r(2) = 0.92). At high concentrations ( > 12 000 nmol/l) bioassay sometimes overestimated DHA. Bioassay of active drug in plasma correlates well with specific chemical analysis by HPLC. ARTS and DHA appear to account for the total antimalarial activity in plasma after ARTS administration. (C) 2003 Elsevier Science B.V. All rights reserved.

U2 - 10.1016/S0001-706X(03)00118-9

DO - 10.1016/S0001-706X(03)00118-9

M3 - Article

VL - 87

SP - 371

EP - 375

JO - Acta Tropica

JF - Acta Tropica

SN - 0001-706X

ER -

Comparison of bioassay and high performance liquid chromatographic assay of artesunate and dihydroartemisinin in plasma

Abstract

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