Comparison of bioassay and high performance liquid chromatographic assay of artesunate and dihydroartemisinin in plasma

B.M. Kotecka, K.H. Rieckmann, Timothy Davis, K.T. Batty, Kenneth Ilett

    Research output: Contribution to journalArticle

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    Abstract

    The study was a comparison of bioassay and HPLC analysis of artesunate (ARTS) and dihydroartemisinin (DHA) in plasma. ARTS and DHA in plasma samples from patients treated with ARTS were quantified by HPLC and expressed as DHA. DHA-equivalents in the same plasma samples were measured using a standardised parasite culture technique. DHA concentrations estimated by both methods were highly correlated (bioassay = 0.96 x HPLC + 11.0; r(2) = 0.92). At high concentrations ( > 12 000 nmol/l) bioassay sometimes overestimated DHA. Bioassay of active drug in plasma correlates well with specific chemical analysis by HPLC. ARTS and DHA appear to account for the total antimalarial activity in plasma after ARTS administration. (C) 2003 Elsevier Science B.V. All rights reserved.
    Original languageEnglish
    Pages (from-to)371-375
    JournalActa Tropica
    Volume87
    DOIs
    Publication statusPublished - 2003

    Fingerprint

    dihydroartemisinin
    Biological Assay
    bioassays
    liquids
    assays
    High Pressure Liquid Chromatography
    antimalarials
    chemical analysis
    Culture Techniques
    parasites
    sampling
    drugs
    Antimalarials
    artesunate
    methodology

    Cite this

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    title = "Comparison of bioassay and high performance liquid chromatographic assay of artesunate and dihydroartemisinin in plasma",
    abstract = "The study was a comparison of bioassay and HPLC analysis of artesunate (ARTS) and dihydroartemisinin (DHA) in plasma. ARTS and DHA in plasma samples from patients treated with ARTS were quantified by HPLC and expressed as DHA. DHA-equivalents in the same plasma samples were measured using a standardised parasite culture technique. DHA concentrations estimated by both methods were highly correlated (bioassay = 0.96 x HPLC + 11.0; r(2) = 0.92). At high concentrations ( > 12 000 nmol/l) bioassay sometimes overestimated DHA. Bioassay of active drug in plasma correlates well with specific chemical analysis by HPLC. ARTS and DHA appear to account for the total antimalarial activity in plasma after ARTS administration. (C) 2003 Elsevier Science B.V. All rights reserved.",
    author = "B.M. Kotecka and K.H. Rieckmann and Timothy Davis and K.T. Batty and Kenneth Ilett",
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    Comparison of bioassay and high performance liquid chromatographic assay of artesunate and dihydroartemisinin in plasma. / Kotecka, B.M.; Rieckmann, K.H.; Davis, Timothy; Batty, K.T.; Ilett, Kenneth.

    In: Acta Tropica, Vol. 87, 2003, p. 371-375.

    Research output: Contribution to journalArticle

    TY - JOUR

    T1 - Comparison of bioassay and high performance liquid chromatographic assay of artesunate and dihydroartemisinin in plasma

    AU - Kotecka, B.M.

    AU - Rieckmann, K.H.

    AU - Davis, Timothy

    AU - Batty, K.T.

    AU - Ilett, Kenneth

    PY - 2003

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    AB - The study was a comparison of bioassay and HPLC analysis of artesunate (ARTS) and dihydroartemisinin (DHA) in plasma. ARTS and DHA in plasma samples from patients treated with ARTS were quantified by HPLC and expressed as DHA. DHA-equivalents in the same plasma samples were measured using a standardised parasite culture technique. DHA concentrations estimated by both methods were highly correlated (bioassay = 0.96 x HPLC + 11.0; r(2) = 0.92). At high concentrations ( > 12 000 nmol/l) bioassay sometimes overestimated DHA. Bioassay of active drug in plasma correlates well with specific chemical analysis by HPLC. ARTS and DHA appear to account for the total antimalarial activity in plasma after ARTS administration. (C) 2003 Elsevier Science B.V. All rights reserved.

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