TY - JOUR
T1 - Comparing the genomes of Helicobacter pylori clinical strain UM032 and Mice-adapted derivatives
AU - Khosravi, Y.
AU - Rehvathy, V.
AU - Wee, W.Y.
AU - Wang, S.
AU - Baybayan, P.
AU - Singh, S.
AU - Ashby, M.
AU - Ong, J.
AU - Amoyo, A.A.
AU - Seow, S.W.
AU - Choo, S.W.
AU - Perkins, Tim
AU - Chua, Eng
AU - Tay, Alfred Chin Yen
AU - Marshall, Barry
AU - Loke, M.F.
AU - Goh, K.L.
AU - Pettersson, S.
AU - Vadivelu, J.
PY - 2013
Y1 - 2013
N2 - Abstract. Background: Helicobacter pylori is a Gram-negative bacterium that persistently infects the human stomach inducing chronic inflammation. The exact mechanisms of pathogenesis are still not completely understood. Although not a natural host for H. pylori, mouse infection models play an important role in establishing the immunology and pathogenicity of H. pylori. In this study, for the first time, the genome sequences of clinical H. pylori strain UM032 and mice-adapted derivatives, 298 and 299, were sequenced using the PacBio Single Molecule, Real-Time (SMRT) technology. Result: Here, we described the single contig which was achieved for UM032 (1,599,441 bp), 298 (1,604,216 bp) and 299 (1,601,149 bp). Preliminary analysis suggested that methylation of H. pylori genome through its restriction modification system may be determinative of its host specificity and adaptation. Conclusion: Availability of these genomic sequences will aid in enhancing our current level of understanding the host specificity of H. pylori. © 2013 Khosravi et al.; licensee BioMed Central Ltd.
AB - Abstract. Background: Helicobacter pylori is a Gram-negative bacterium that persistently infects the human stomach inducing chronic inflammation. The exact mechanisms of pathogenesis are still not completely understood. Although not a natural host for H. pylori, mouse infection models play an important role in establishing the immunology and pathogenicity of H. pylori. In this study, for the first time, the genome sequences of clinical H. pylori strain UM032 and mice-adapted derivatives, 298 and 299, were sequenced using the PacBio Single Molecule, Real-Time (SMRT) technology. Result: Here, we described the single contig which was achieved for UM032 (1,599,441 bp), 298 (1,604,216 bp) and 299 (1,601,149 bp). Preliminary analysis suggested that methylation of H. pylori genome through its restriction modification system may be determinative of its host specificity and adaptation. Conclusion: Availability of these genomic sequences will aid in enhancing our current level of understanding the host specificity of H. pylori. © 2013 Khosravi et al.; licensee BioMed Central Ltd.
U2 - 10.1186/1757-4749-5-25
DO - 10.1186/1757-4749-5-25
M3 - Article
C2 - 23957912
VL - 5
SP - 6pp
JO - Gut Pathogens
JF - Gut Pathogens
SN - 1757-4749
IS - 1
ER -