Comparative studies of the immunogenicity and protective potential of biofilm vs planktonic Staphylococcus aureus vaccine against bovine mastitis using non-invasive mouse mastitis as a model system

Jully Gogoi-Tiwari, Vincent Williams, Charlene Babra Waryah, Karina Yui Eto, Modiri Tau, Paul Costantino, Harish Kumar Tiwari, Trilochan Mukkur

Research output: Contribution to journalArticlepeer-review

22 Citations (Scopus)

Abstract

This study was undertaken to compare the immunogenicity and protective potential of biofilm vs planktonic Staphylococcus aureus vaccine for the prevention of mastitis using the mouse as a model system. Mice immunized with formalin-killed whole cell vaccine of S. aureus residing in a biofilm when delivered via an intramammary route produced a cell mediated immune response. Mice immunized with this biofilm vaccine showed significant reductions in colonization by S. aureus in mammary glands, severity of clinical symptoms and tissue damage in mammary glands in comparison with the mice immunized with formalin-killed whole cells of planktonic S. aureus. The planktonic vaccine administered by a subcutaneous route produced a significantly higher humoral immune response (IgG1 and IgG) than the biofilm vaccine. However, considering the host response, tissue damage, the clinical severity and colonization of S. aureus in mammary glands, the biofilm vaccine performed better in immunogenicity and protective potential when administered by the intramammary route.

Original languageEnglish
Pages (from-to)543-554
Number of pages12
JournalBiofouling
Volume31
Issue number7
DOIs
Publication statusPublished - 9 Aug 2015
Externally publishedYes

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