A butenolide (3-methyl-2H-furo[2,3-c ]pyran-2-one, the phyto-active compound isolated from smoke) was tested for itseffect on somatic embryogenesis with an important species for commercial horticulture, Baloskion tetraphyllum. Whensomatic embryos of B. tetraphyllum were transferred to basal medium supplemented with butenolide, enhanceddevelopment of growth-competent somatic embryos was observed. This study demonstrates that butenolide exhibitspromotive effects on both germination of seed (stimulation of zygotic embryos), and stimulation of development andmaturation of somatic embryos with B. tetraphyllum, providing a potential new class of phyto-active compounds for in vitroculture.
Ma, C., Bunn, E., Dixon, K., & Flematti, G. (2006). Comparative Enhancement of Germination and Vigor in Seed and Somatic Embryos by the Smoke Chemical 3-Methyl-2H-FURO[2,3-C]PYRAN-2-ONE in Baloskion Tetraphyllum (Restionaceae). In Vitro Cellular & Development Biology - Plant, 42(3), 305-308. https://doi.org/10.1079/IVP2006758