Comparative colonisation by virulent versus avirulent Pyricularia oryzae on wild Oryza australiensis

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Abstract

Pyricularia oryzae (rice blast) conidial development at pre-penetration stage determines success or otherwise of infection inside the rice host plants. Studies on conidial germination and growth on the leaf surface in commercial rice (Oryza sativa) report differently, dependent upon host type and level of blast resistance. Although wild rice (O. australiensis) is known to be an alternative host of blast, the interaction between P. oryzae conidia and wild O. australiensis on its leaf surface has not been previously studied. We found significant (P < 0.001) differences in conidial development between two blast isolates with different virulence in terms of conidial germination, germ tube growth and appressoria formation on both wild and cultivated rice. Conidial germination at 6 h post-inoculation (hpi) for the virulent isolate was significantly (P < 0.001) delayed. Germ tubes of the avirulent isolate conidia grew significantly (P < 0.001) faster and with significantly (P < 0.001) longer germ tubes than from virulent conidia. Appressoria development for the virulent isolate was significantly (P < 0.001) faster at its later growth stages of 12 and 18 hpi when approximately 100% of germ tubes formed appressoria. In contrast, formation rate of appressoria for the avirulent isolate was significantly (P < 0.001) slower and only reached 76% of germ tubes forming appressoria. Appressoria formation on O. australiensis was significantly (P < 0.001) greater than the formation on O. sativa for both virulent and avirulent P. oryzae at 12 hpi, a clear indication that host type influences the extent of appressoria formation.

Original languageEnglish
Pages (from-to)927-936
Number of pages10
JournalEuropean Journal of Plant Pathology
Volume151
Issue number4
DOIs
Publication statusPublished - 1 Aug 2018

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Oryza australiensis
Magnaporthe oryzae
appressoria
germ tube
conidia
avirulent strains
germination
rice
Oryza sativa
alternative hosts
wild rice
blast disease
leaves
virulence
host plants
developmental stages

Cite this

@article{9791a37aca7b4aa0b0613b9d66e3f77d,
title = "Comparative colonisation by virulent versus avirulent Pyricularia oryzae on wild Oryza australiensis",
abstract = "Pyricularia oryzae (rice blast) conidial development at pre-penetration stage determines success or otherwise of infection inside the rice host plants. Studies on conidial germination and growth on the leaf surface in commercial rice (Oryza sativa) report differently, dependent upon host type and level of blast resistance. Although wild rice (O. australiensis) is known to be an alternative host of blast, the interaction between P. oryzae conidia and wild O. australiensis on its leaf surface has not been previously studied. We found significant (P < 0.001) differences in conidial development between two blast isolates with different virulence in terms of conidial germination, germ tube growth and appressoria formation on both wild and cultivated rice. Conidial germination at 6 h post-inoculation (hpi) for the virulent isolate was significantly (P < 0.001) delayed. Germ tubes of the avirulent isolate conidia grew significantly (P < 0.001) faster and with significantly (P < 0.001) longer germ tubes than from virulent conidia. Appressoria development for the virulent isolate was significantly (P < 0.001) faster at its later growth stages of 12 and 18 hpi when approximately 100{\%} of germ tubes formed appressoria. In contrast, formation rate of appressoria for the avirulent isolate was significantly (P < 0.001) slower and only reached 76{\%} of germ tubes forming appressoria. Appressoria formation on O. australiensis was significantly (P < 0.001) greater than the formation on O. sativa for both virulent and avirulent P. oryzae at 12 hpi, a clear indication that host type influences the extent of appressoria formation.",
keywords = "Appressorium, Pre-penetration, Rice blast, Wild rice",
author = "Dolar Pak and You, {Ming Pei} and Vincent Lanoiselet and Barbetti, {Martin J.}",
year = "2018",
month = "8",
day = "1",
doi = "10.1007/s10658-018-1428-5",
language = "English",
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pages = "927--936",
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TY - JOUR

T1 - Comparative colonisation by virulent versus avirulent Pyricularia oryzae on wild Oryza australiensis

AU - Pak, Dolar

AU - You, Ming Pei

AU - Lanoiselet, Vincent

AU - Barbetti, Martin J.

PY - 2018/8/1

Y1 - 2018/8/1

N2 - Pyricularia oryzae (rice blast) conidial development at pre-penetration stage determines success or otherwise of infection inside the rice host plants. Studies on conidial germination and growth on the leaf surface in commercial rice (Oryza sativa) report differently, dependent upon host type and level of blast resistance. Although wild rice (O. australiensis) is known to be an alternative host of blast, the interaction between P. oryzae conidia and wild O. australiensis on its leaf surface has not been previously studied. We found significant (P < 0.001) differences in conidial development between two blast isolates with different virulence in terms of conidial germination, germ tube growth and appressoria formation on both wild and cultivated rice. Conidial germination at 6 h post-inoculation (hpi) for the virulent isolate was significantly (P < 0.001) delayed. Germ tubes of the avirulent isolate conidia grew significantly (P < 0.001) faster and with significantly (P < 0.001) longer germ tubes than from virulent conidia. Appressoria development for the virulent isolate was significantly (P < 0.001) faster at its later growth stages of 12 and 18 hpi when approximately 100% of germ tubes formed appressoria. In contrast, formation rate of appressoria for the avirulent isolate was significantly (P < 0.001) slower and only reached 76% of germ tubes forming appressoria. Appressoria formation on O. australiensis was significantly (P < 0.001) greater than the formation on O. sativa for both virulent and avirulent P. oryzae at 12 hpi, a clear indication that host type influences the extent of appressoria formation.

AB - Pyricularia oryzae (rice blast) conidial development at pre-penetration stage determines success or otherwise of infection inside the rice host plants. Studies on conidial germination and growth on the leaf surface in commercial rice (Oryza sativa) report differently, dependent upon host type and level of blast resistance. Although wild rice (O. australiensis) is known to be an alternative host of blast, the interaction between P. oryzae conidia and wild O. australiensis on its leaf surface has not been previously studied. We found significant (P < 0.001) differences in conidial development between two blast isolates with different virulence in terms of conidial germination, germ tube growth and appressoria formation on both wild and cultivated rice. Conidial germination at 6 h post-inoculation (hpi) for the virulent isolate was significantly (P < 0.001) delayed. Germ tubes of the avirulent isolate conidia grew significantly (P < 0.001) faster and with significantly (P < 0.001) longer germ tubes than from virulent conidia. Appressoria development for the virulent isolate was significantly (P < 0.001) faster at its later growth stages of 12 and 18 hpi when approximately 100% of germ tubes formed appressoria. In contrast, formation rate of appressoria for the avirulent isolate was significantly (P < 0.001) slower and only reached 76% of germ tubes forming appressoria. Appressoria formation on O. australiensis was significantly (P < 0.001) greater than the formation on O. sativa for both virulent and avirulent P. oryzae at 12 hpi, a clear indication that host type influences the extent of appressoria formation.

KW - Appressorium

KW - Pre-penetration

KW - Rice blast

KW - Wild rice

UR - http://www.scopus.com/inward/record.url?scp=85045025594&partnerID=8YFLogxK

U2 - 10.1007/s10658-018-1428-5

DO - 10.1007/s10658-018-1428-5

M3 - Article

VL - 151

SP - 927

EP - 936

JO - European Journal of Plant Pathology

JF - European Journal of Plant Pathology

SN - 0929-1873

IS - 4

ER -