Comparative analysis of human and marsupial Leishmania: Characterisation of Leishmania australiensis, development of a potential human attenuated vaccine and assessment of vector competence

Calila Sena Santos

doi:10.26182/5b4c0ad4a6990

Comparative analysis of human and marsupial Leishmania: Characterisation of Leishmania australiensis, development of a potential human attenuated vaccine and assessment of vector competence

Calila Sena Santos

Research output: Thesis › Doctoral Thesis

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Abstract

Leishmania parasites are protozoans able to infect many vertebrates including humans. Leishmania macropodum causes leishmaniasis in Australian marsupials. This study examined Leishmania macropodum in vitro life cycle, resistance to complement mediate lysis and in vivo responses to infection in the mouse model. It utilised an experimental murine infection system to examine the possible use of Leishmania macropodum as a safe, attenuated vaccine against human leishmaniasis. Furthermore, using in silico analysis, the main receptors used in parasite-vector interaction were analysed. Leishmania macropodum is resistant to normal mouse serum and can cause transient infection in mice. These and other results are discussed.

Original language	English
Qualification	Doctor of Philosophy
Awarding Institution	The University of Western Australia
Supervisors/Advisors	Peacock, Christopher, Supervisor Beilharz, Manfred, Supervisor
Award date	5 Jul 2018
DOIs	https://doi.org/10.26182/5b4c0ad4a6990
Publication status	Unpublished - 2018

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10.26182/5b4c0ad4a6990

THESIS - DOCTOR OF PHILOSOPHY - SENA SANTOS Calila Sena - 2018
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Cite this

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title = "Comparative analysis of human and marsupial Leishmania: Characterisation of Leishmania australiensis, development of a potential human attenuated vaccine and assessment of vector competence",

abstract = "Leishmania parasites are protozoans able to infect many vertebrates including humans. Leishmania macropodum causes leishmaniasis in Australian marsupials. This study examined Leishmania macropodum in vitro life cycle, resistance to complement mediate lysis and in vivo responses to infection in the mouse model. It utilised an experimental murine infection system to examine the possible use of Leishmania macropodum as a safe, attenuated vaccine against human leishmaniasis. Furthermore, using in silico analysis, the main receptors used in parasite-vector interaction were analysed. Leishmania macropodum is resistant to normal mouse serum and can cause transient infection in mice. These and other results are discussed.",

keywords = "Marsupial Leishmania, Leishmania macropodum, Mouse experimental infection, Live-attenuated vaccines, Complement resistance, Cloning and Transfection, Insect vector competency, Life cycle",

author = "Santos, {Calila Sena}",

year = "2018",

doi = "10.26182/5b4c0ad4a6990",

language = "English",

school = "The University of Western Australia",

}

TY - BOOK

T1 - Comparative analysis of human and marsupial Leishmania: Characterisation of Leishmania australiensis, development of a potential human attenuated vaccine and assessment of vector competence

AU - Santos, Calila Sena

PY - 2018

Y1 - 2018

N2 - Leishmania parasites are protozoans able to infect many vertebrates including humans. Leishmania macropodum causes leishmaniasis in Australian marsupials. This study examined Leishmania macropodum in vitro life cycle, resistance to complement mediate lysis and in vivo responses to infection in the mouse model. It utilised an experimental murine infection system to examine the possible use of Leishmania macropodum as a safe, attenuated vaccine against human leishmaniasis. Furthermore, using in silico analysis, the main receptors used in parasite-vector interaction were analysed. Leishmania macropodum is resistant to normal mouse serum and can cause transient infection in mice. These and other results are discussed.

AB - Leishmania parasites are protozoans able to infect many vertebrates including humans. Leishmania macropodum causes leishmaniasis in Australian marsupials. This study examined Leishmania macropodum in vitro life cycle, resistance to complement mediate lysis and in vivo responses to infection in the mouse model. It utilised an experimental murine infection system to examine the possible use of Leishmania macropodum as a safe, attenuated vaccine against human leishmaniasis. Furthermore, using in silico analysis, the main receptors used in parasite-vector interaction were analysed. Leishmania macropodum is resistant to normal mouse serum and can cause transient infection in mice. These and other results are discussed.

KW - Marsupial Leishmania

KW - Leishmania macropodum

KW - Mouse experimental infection

KW - Live-attenuated vaccines

KW - Complement resistance

KW - Cloning and Transfection

KW - Insect vector competency

KW - Life cycle

U2 - 10.26182/5b4c0ad4a6990

DO - 10.26182/5b4c0ad4a6990

M3 - Doctoral Thesis

ER -

Comparative analysis of human and marsupial Leishmania: Characterisation of Leishmania australiensis, development of a potential human attenuated vaccine and assessment of vector competence

Abstract

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