CMV drives the expansion of highly functional memory T cells expressing NK-cell receptors in renal transplant recipients

Nandini Makwana, Bree Foley, Sonia Fernandez, Silvia Lee, Ashley Irish, Hanspeter Pircher, Patricia Price

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Cytomegalovirus (CMV) is a common opportunistic infection encountered in renal transplant recipients (RTRs) and may be reactivated without symptoms at any time post-transplant. We describe how active and latent CMV affect T-cell subsets in RTRs who are stable on maintenance therapy. T-cell responses to CMV were assessed in RTRs (n = 54) >2 years post-transplant, and healthy controls (n = 38). Seven RTRs had CMV DNA detectable in plasma. CMV antibody and DNA aligned with increased proportions of CD8+ T cells and reduced CD4/CD8 ratios. This paralleled an expansion of effector memory T-cell (TEM), terminally differentiated T-cell (TEMRA) and CD57+ TEMRA cell populations. Expression of NK-cell receptors, LIR-1 and KLRG1 on CD4+ and CD8+ CD57+ TEM and TEMRA cells correlated with elevated interferon-γ and cytotoxic responses to anti-CD3 and increased cytotoxic responses to CMV phosphoprotein (pp) 65 in RTRs who carried CMV DNA. CD8+ T cells from all CMV seropositive RTRs responded efficiently to CMV immediate early (IE) -1 peptides. The data show that latent and active CMV infection can alter T-cell subsets in RTRs many years after transplantation, and up-regulate T-cell expression of NK-cell receptors. This may enhance effector responses of CD4+ and CD8+ T cells against CMV.

Original languageEnglish
Pages (from-to)1324-1334
Number of pages11
JournalEuropean Journal of Immunology
Volume47
Issue number8
DOIs
Publication statusPublished - 1 Aug 2017

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Natural Killer Cell Receptors
Cytomegalovirus
T-Lymphocytes
Kidney
T-Lymphocyte Subsets
DNA
Transplants
Transplant Recipients
CD4-CD8 Ratio
Opportunistic Infections
Cytomegalovirus Infections
Interferons
Up-Regulation
Transplantation

Cite this

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title = "CMV drives the expansion of highly functional memory T cells expressing NK-cell receptors in renal transplant recipients",
abstract = "Cytomegalovirus (CMV) is a common opportunistic infection encountered in renal transplant recipients (RTRs) and may be reactivated without symptoms at any time post-transplant. We describe how active and latent CMV affect T-cell subsets in RTRs who are stable on maintenance therapy. T-cell responses to CMV were assessed in RTRs (n = 54) >2 years post-transplant, and healthy controls (n = 38). Seven RTRs had CMV DNA detectable in plasma. CMV antibody and DNA aligned with increased proportions of CD8+ T cells and reduced CD4/CD8 ratios. This paralleled an expansion of effector memory T-cell (TEM), terminally differentiated T-cell (TEMRA) and CD57+ TEMRA cell populations. Expression of NK-cell receptors, LIR-1 and KLRG1 on CD4+ and CD8+ CD57+ TEM and TEMRA cells correlated with elevated interferon-γ and cytotoxic responses to anti-CD3 and increased cytotoxic responses to CMV phosphoprotein (pp) 65 in RTRs who carried CMV DNA. CD8+ T cells from all CMV seropositive RTRs responded efficiently to CMV immediate early (IE) -1 peptides. The data show that latent and active CMV infection can alter T-cell subsets in RTRs many years after transplantation, and up-regulate T-cell expression of NK-cell receptors. This may enhance effector responses of CD4+ and CD8+ T cells against CMV.",
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CMV drives the expansion of highly functional memory T cells expressing NK-cell receptors in renal transplant recipients. / Makwana, Nandini; Foley, Bree; Fernandez, Sonia; Lee, Silvia; Irish, Ashley; Pircher, Hanspeter; Price, Patricia.

In: European Journal of Immunology, Vol. 47, No. 8, 01.08.2017, p. 1324-1334.

Research output: Contribution to journalArticle

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AU - Makwana, Nandini

AU - Foley, Bree

AU - Fernandez, Sonia

AU - Lee, Silvia

AU - Irish, Ashley

AU - Pircher, Hanspeter

AU - Price, Patricia

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