TY - JOUR
T1 - CLB19, a pentatricopeptide repeat protein required for editing of rpoA and clpP chloroplast transcripts
AU - Chateigner-Boutin, Anne-Laure
AU - Ramos-Vega, M.
AU - Guevara-Garcia, A.
AU - Andres, C.
AU - De La Luz Gutierrez-Nava, M.
AU - Cantero, A
AU - Delannoy, Etienne
AU - Jiminez, L.F.
AU - Lurin, C.
AU - Small, Ian
AU - Leo, P.
PY - 2008
Y1 - 2008
N2 - RNA editing changes the sequence of many transcripts in plant organelles, but little is known about the molecular mechanisms determining the specificity of the process. In this study, we have characterized CLB19 (also known as PDE247), a gene that is required for editing of two distinct chloroplast transcripts, rpoA and clpP. Loss-of-function clb19 mutants present a yellow phenotype with impaired chloroplast development and early seedling lethality under greenhouse conditions. Transcript patterns are profoundly affected in the mutant plants, with a pattern entirely consistent with a defect in activity of the plastid-encoded RNA polymerase. CLB19 encodes a pentatricopeptide repeat protein similar to the editing specificity factors CRR4 and CRR21, but, unlike them, is implicated in editing of two target sites.
AB - RNA editing changes the sequence of many transcripts in plant organelles, but little is known about the molecular mechanisms determining the specificity of the process. In this study, we have characterized CLB19 (also known as PDE247), a gene that is required for editing of two distinct chloroplast transcripts, rpoA and clpP. Loss-of-function clb19 mutants present a yellow phenotype with impaired chloroplast development and early seedling lethality under greenhouse conditions. Transcript patterns are profoundly affected in the mutant plants, with a pattern entirely consistent with a defect in activity of the plastid-encoded RNA polymerase. CLB19 encodes a pentatricopeptide repeat protein similar to the editing specificity factors CRR4 and CRR21, but, unlike them, is implicated in editing of two target sites.
U2 - 10.1111/j.1365-313X.2008.03634.x
DO - 10.1111/j.1365-313X.2008.03634.x
M3 - Article
C2 - 18657233
SN - 0960-7412
VL - 56
SP - 590
EP - 602
JO - The Plant Journal
JF - The Plant Journal
IS - 4
ER -