TY - JOUR
T1 - Chylomicron remnant metabolism in familial dyslipidemias studied with a remnant-like emulsion breath test
AU - Redgrave, T.G.
AU - Watts, Gerald
AU - Martins, I.J.
AU - Barrett, Hugh
AU - Mamo, John
AU - Dimmitt, Simon
AU - Marais, A.D.
PY - 2001
Y1 - 2001
N2 - We have developed a stable isotope breath test for the assessment of chylomicron remnant metabolism and report the results from the breath test in human subjects selected for disorders of chylomicron or remnant metabolism. In type I hyperlipemia, the phenotype is extreme hypertriglyceridemia due to a lack of lipoprotein lipase activity, which causes the failure of remnant formation, The type UI dyslipidemia phenotype is caused by the inefficient removal of chylomicron remnants from plasma, generally because of homozygosity for apolipoprotein E2 alleles. The breath test was predicted to be abnormal in type m hyperlipemia, whereas a priori in type I hyperlipemia defective remnant clearance was not anticipated. Subjects were injected with lipid emulsions prepared with a composition similar to normal chylomicron remnants. The emulsions contained cholesteryl ester incorporating the stable nonradioactive isotope C-13 in the fatty acid moiety, End exhalation breath was collected at intervals after intravenous injection of the remnant-like emulsions and analyzed for C-13 enrichment by isotope-ratio mass spectrometry. Compared with the group of normolipemic men, the fractional catabolic rate of remnants measured by the breath test was significantly decreased (P = 0.006) in subjects with type m dyslipidemia. In the group with type I hyperlipemia, the fractional catabolic rate was not different (P = 0.233) from the control group, Therefore, the underlying capacity for remnant catabolism j/r normal in this group of markedly hypertriglyceridemic subjects.j/r By shortcircuiting the step of lipolysis, the remnant-like emulsion breath test provides direct information about remnant clearance and metabolism, which should assist in investigations of postprandial lipid metabolism.-Redgrave, T G., G. F. Watts, I. J. Martins, P. H. R. Barrett, J. C. L. Mamo, S. B. Dimmitt, and A. D. Marais. Chylomicron remnant metabolism in familial dyslipidemias studied with a remnant-like emulsion breath test.
AB - We have developed a stable isotope breath test for the assessment of chylomicron remnant metabolism and report the results from the breath test in human subjects selected for disorders of chylomicron or remnant metabolism. In type I hyperlipemia, the phenotype is extreme hypertriglyceridemia due to a lack of lipoprotein lipase activity, which causes the failure of remnant formation, The type UI dyslipidemia phenotype is caused by the inefficient removal of chylomicron remnants from plasma, generally because of homozygosity for apolipoprotein E2 alleles. The breath test was predicted to be abnormal in type m hyperlipemia, whereas a priori in type I hyperlipemia defective remnant clearance was not anticipated. Subjects were injected with lipid emulsions prepared with a composition similar to normal chylomicron remnants. The emulsions contained cholesteryl ester incorporating the stable nonradioactive isotope C-13 in the fatty acid moiety, End exhalation breath was collected at intervals after intravenous injection of the remnant-like emulsions and analyzed for C-13 enrichment by isotope-ratio mass spectrometry. Compared with the group of normolipemic men, the fractional catabolic rate of remnants measured by the breath test was significantly decreased (P = 0.006) in subjects with type m dyslipidemia. In the group with type I hyperlipemia, the fractional catabolic rate was not different (P = 0.233) from the control group, Therefore, the underlying capacity for remnant catabolism j/r normal in this group of markedly hypertriglyceridemic subjects.j/r By shortcircuiting the step of lipolysis, the remnant-like emulsion breath test provides direct information about remnant clearance and metabolism, which should assist in investigations of postprandial lipid metabolism.-Redgrave, T G., G. F. Watts, I. J. Martins, P. H. R. Barrett, J. C. L. Mamo, S. B. Dimmitt, and A. D. Marais. Chylomicron remnant metabolism in familial dyslipidemias studied with a remnant-like emulsion breath test.
M3 - Article
SN - 0022-2275
VL - 42
SP - 710
EP - 715
JO - Journal of Lipid Research
JF - Journal of Lipid Research
IS - 5
ER -