Chronic kidney disease delays VLDL-apoB-100 particle catabolism : potential role of apolipoprotein C-III

Doris Chan, Gursharan Dogra, A.B. Irish, Esther Ooi, Hugh Barrett, Dick Chan, Gerald Watts

    Research output: Contribution to journalArticle

    42 Citations (Scopus)

    Abstract

    To determine the relative contribution of obesity and/or insulin resistance (IR) in the development of dyslipidemia in chronic kidney disease (CKD), we investigated the transport of apolipoprotein (apo) B-100 in nonobese, nondiabetic, nonnephrotic CKD subjects and healthy controls (HC). We determined total VLDL, VLDL1, VLDL2, intermediate density lipoprotein (IDL), and LDL-apoB-100 using intravenous D3-leucine, GC-MS, and multicompartmental modeling. Plasma apoC-III and apoB-48 were immunoassayed. In this case control study, we report higher plasma triglyceride, IDL-, VLDL-, VLDL1-, and VLDL2-apoB-100 concentrations in CKD compared with HC (P <0.05). This was associated with decreased fractional catabolic rates [FCRs (pools/day)] [IDL:CKD 3.4 (1.6) vs. HC 5.0 (3.2), P <0.0001; VLDL:CKD 4.8 (5.2) vs. HC 7.8 (4.8), P = 0.038; VLDL1:CKD 10.1 (8.5) vs. HC 29.5 (45.1), P = 0.007; VLDL2:CKD 5.4 (4.6) vs. HC 10.4 (3.4), P = 0.001] with no difference in production rates. Plasma apoC-III and apoB-48 were significantly higher in CKD (P <0.001) and both correlated with impaired FCRs of VLDL, VLDL1, and VLDL2 apoB-100 (P <0.05). In CKD, apoC-III concentration was the only independent predictor of clearance defects in VLDL and its subfractions. Moderate CKD in the absence of central adiposity and IR is associated with mild hypertriglyceridemia due to delayed catabolism of triglyceride rich lipoproteins, IDL, and VLDL, without changes in production rate. Altered apoC-III metabolism may contribute to dyslipidemia in CKD, and this requires further investigation.
    Original languageEnglish
    Pages (from-to)2524-2531
    JournalJournal of Lipid Research
    Volume50
    Issue number12
    DOIs
    Publication statusPublished - 2009

    Fingerprint Dive into the research topics of 'Chronic kidney disease delays VLDL-apoB-100 particle catabolism : potential role of apolipoprotein C-III'. Together they form a unique fingerprint.

    Cite this