Chloroplast DNA Copy Number May Link to Sex Determination in Leucadendron (Protoeaceae)

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    Leucadendron (Proteaceae) is a South African genus, the flowers of which have become a popular item in theAustralian cut-flower industry. All species are dioecious. In general the female flowers are the more desirable as cutflowers. The availability of a molecular marker linked to sex determination is therefore needed both to maximize theefficiency of breeding programs and to supply markets with flowers from the preferred sex. The polymerase chainreaction-based method of suppression subtractive hybridization (SSH) combined with mirror orientation selection (MOS)were applied in an attempt to identify genome differences between male and female plants of Leucadendron discolor.Screening of 416 clones from a male-subtracted genomic DNA library and 282 clones from a female-subtracted libraryidentified 13 candidates for male-specific genomic fragments. Sequence analyses of the 13 candidate DNA fragmentsshowed that they were fragments of the chloroplast DNA, raising the possibility that chloroplast DNA copy number islinked to sex determination in Leucadendron.
    Original languageEnglish
    Pages (from-to)21-24
    JournalHAYATI Journal of Biosciences
    Issue number1
    Publication statusPublished - 2009


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