Characterizing the reproductive biology of the female pygmy hippopotamus (Choeropsis liberiensis) through non-invasive endocrine monitoring

Gabriella L. Flacke, Franz Schwarzenberger, Linda M. Penfold, Susan L. Walker, Graeme B. Martin, Robert Peter Millar, Monique C.J. Paris

    Research output: Contribution to journalArticle

    3 Citations (Scopus)

    Abstract

    The pygmy hippopotamus (Choeropsis liberiensis) is endangered in the wild and very little is known about its reproductive biology. In zoological facilities, this species experiences a number of reproductive issues that complicate breeding management, including a high rate of stillbirths and failure of many pairs to reproduce. We conducted a comprehensive study to evaluate reproductive cycles and pregnancy in this species using enzyme immunoassays (EIAs) for fecal hormone metabolite analysis. Fresh fecal samples were collected twice weekly for a one to three year period from 36 female pygmy hippos housed at 24 zoological institutions. Samples were analyzed in three separate laboratories. Three progestogen metabolite EIAs (Pg-diol: 5β-pregnane-3α,20α-diol 3HS:BSA; PdG: pregnanediol-3-glucuronide R13904; mono-P4: Quidel clone 425) and three estrogen metabolite EIAs (E2a: estradiol-17β-OH 17-HS:BSA; E2b: estradiol 17β R0008; E2c: estradiol 17β R4972) accurately reflected reproductive events. Average estrous cycle length was 31.8 ± 7.4 days based on estrogen metabolite peaks and 30.9 ± 7.3 days based on nadir to nadir progestogen metabolite concentrations. Cyclical patterns in both estrogen and progestogen metabolites were detected throughout the year, indicating a lack of seasonality. Estrogen metabolite peaks were also observed during pregnancy and lactation, suggesting that follicular development occurs during both reproductive states. Pregnancy was most reliably demonstrated by elevation in progestogen metabolites (Pg-diol or PdG) in the second half of gestation. Average gestation length based on breeding to calving date was 203 ± 4 days for 15 pregnancies. This comprehensive overview of the reproductive biology of the female pygmy hippo provides valuable data for guiding long-term breeding management for this endangered species and serves as a baseline for future studies addressing the potential influence of social structure, diet, body condition, and other husbandry factors on estrous cycling and reproduction.

    Original languageEnglish
    Pages (from-to)126-138
    Number of pages13
    JournalTheriogenology
    Volume102
    Early online date19 Jul 2017
    DOIs
    Publication statusPublished - 15 Oct 2017

    Fingerprint

    Artiodactyla
    Progestins
    metabolites
    Biological Sciences
    Pregnancy
    monitoring
    progestational hormones
    Estrogens
    pregnancy
    Immunoenzyme Techniques
    glycols
    estrogens
    Breeding
    Estradiol
    enzyme immunoassays
    estradiol
    Pregnanes
    Endangered Species
    breeding
    Stillbirth

    Cite this

    Flacke, Gabriella L. ; Schwarzenberger, Franz ; Penfold, Linda M. ; Walker, Susan L. ; Martin, Graeme B. ; Millar, Robert Peter ; Paris, Monique C.J. / Characterizing the reproductive biology of the female pygmy hippopotamus (Choeropsis liberiensis) through non-invasive endocrine monitoring. In: Theriogenology. 2017 ; Vol. 102. pp. 126-138.
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    abstract = "The pygmy hippopotamus (Choeropsis liberiensis) is endangered in the wild and very little is known about its reproductive biology. In zoological facilities, this species experiences a number of reproductive issues that complicate breeding management, including a high rate of stillbirths and failure of many pairs to reproduce. We conducted a comprehensive study to evaluate reproductive cycles and pregnancy in this species using enzyme immunoassays (EIAs) for fecal hormone metabolite analysis. Fresh fecal samples were collected twice weekly for a one to three year period from 36 female pygmy hippos housed at 24 zoological institutions. Samples were analyzed in three separate laboratories. Three progestogen metabolite EIAs (Pg-diol: 5β-pregnane-3α,20α-diol 3HS:BSA; PdG: pregnanediol-3-glucuronide R13904; mono-P4: Quidel clone 425) and three estrogen metabolite EIAs (E2a: estradiol-17β-OH 17-HS:BSA; E2b: estradiol 17β R0008; E2c: estradiol 17β R4972) accurately reflected reproductive events. Average estrous cycle length was 31.8 ± 7.4 days based on estrogen metabolite peaks and 30.9 ± 7.3 days based on nadir to nadir progestogen metabolite concentrations. Cyclical patterns in both estrogen and progestogen metabolites were detected throughout the year, indicating a lack of seasonality. Estrogen metabolite peaks were also observed during pregnancy and lactation, suggesting that follicular development occurs during both reproductive states. Pregnancy was most reliably demonstrated by elevation in progestogen metabolites (Pg-diol or PdG) in the second half of gestation. Average gestation length based on breeding to calving date was 203 ± 4 days for 15 pregnancies. This comprehensive overview of the reproductive biology of the female pygmy hippo provides valuable data for guiding long-term breeding management for this endangered species and serves as a baseline for future studies addressing the potential influence of social structure, diet, body condition, and other husbandry factors on estrous cycling and reproduction.",
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    Characterizing the reproductive biology of the female pygmy hippopotamus (Choeropsis liberiensis) through non-invasive endocrine monitoring. / Flacke, Gabriella L.; Schwarzenberger, Franz; Penfold, Linda M.; Walker, Susan L.; Martin, Graeme B.; Millar, Robert Peter; Paris, Monique C.J.

    In: Theriogenology, Vol. 102, 15.10.2017, p. 126-138.

    Research output: Contribution to journalArticle

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    AU - Schwarzenberger, Franz

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