Increased production of prostaglandins and cytokines by amnion, particularly prostaglandin (PG) E-2, interleukin (IL)-6 and IL-8, is thought to be an important event in infection-associated preterm labour. We characterized the amnion-derived AV3 cell line to determine its appropriateness as a model for investigation of the regulation of amnion cytokine and PG production. Amnion-derived AV3 cells were treated with tumour necrosis factor-alpha (TNF-alpha, interleukin-1 beta (IL-1 beta), epidermal growth factor (EGF) and phorbol 12-myristate 13-acetate (PMA) and IL-6, IL-8 and prostaglandin production was determined by immunoassay. Production of IL-6 and IL-8 rose dramatically with all treatments. PGE(2), but not PGF(2 alpha) or 6-keto- PGF(1 alpha), biosynthesis was also increased in a concentration- dependent manner with all treatments. A rapid increase in PGHS-2 (but not PGHS-1) mRNA expression was observed in response to TNF-alpha and IL-1 beta. We conclude that the AV3 cell line inflammatory response profile is similar to those observed in primary amnion and other amnion-derived cell lines, and is an appropriate model for human amnion. (C) 1999 Harcourt Publishers Ltd.
|Journal||Prostaglandins, Leukotrienes and Essential Fatty Acids|
|Publication status||Published - 1999|