Characterization of simple sequence repeat markers derived in silico from Brassica rapa bacterial artificial chromosome sequences and their application in Brassica napus: Primer note

Alison E. Ling, Jatinder Kaur, Benjamin Burgess, Melanie Hand, Clare J. Hopkins, Xi Li, Christopher G. Love, Megan Vardy, Marzena Walkiewicz, German Spangenberg, David Edwards, Jacqueline Batley

Research output: Contribution to journalReview article

26 Citations (Scopus)

Abstract

A collaborative Brassica rapa genome sequencing project is currently in progress to aid the identification of agronomically important traits in Brassica species. As an initial stage, the ends of over 110 000 bacterial artificial chromosome clones were sequenced and mined for simple sequence repeats (SSRs). We present the characterization of 40 of these SSRs and their application in Brassica napus. The markers were screened against six Brassica species and Arabidopsis, and demonstrated reliable amplification, genome specificity, cross-amplification and significant polymorphism. These SSRs will be useful for genetic analysis of Brassica germplasm.

Original languageEnglish
Pages (from-to)273-277
Number of pages5
JournalMolecular Ecology Notes
Volume7
Issue number2
DOIs
Publication statusPublished - 1 Mar 2007
Externally publishedYes

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