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Abstract
Aim: To characterize multiscale interactions between high intensity focused ultrasound (HIFU) and dentin collagen and associated matrix-metalloproteinases; in addition, to analyse effect of HIFU on bacterial biofilms and biological properties. Materials and Methods: Dentin specimens were subjected to 10 or 20 s HIFU. XPS-spectra were acquired and TEM performed on dentin slabs. Collagen orientation was performed using Raman spectroscopy. Calcium measurements in human dental pulpal cells (hDPCs) were carried out after 7 and 14 days. For macrophages, CD36+ and CD163 were analysed. Biofilms were analyzed using CLSM. Tandem Mass spectroscopy was performed for detection of hydroxyproline sequences along with human MMP-2 quantification. Results: Phosphorous, calcium, and nitrogen were detected in HIFU specimens. TEM images demonstrated collagen network appearing fused together in HIFU 10 and 20 s specimens. Band associated with 960 cm-1 corresponds to stretching ν1 PO43-. Control specimens showed intensive calcium staining followed by HIFU 20 s>HIFU 10 s>HIFU 5 s specimens. Macrophages in HIFU specimens co-expressed CD80+ and CD 163+ cells. CLSM images showed HIFU treatment inhibiting bacterial growth. SiteScore propensity determined effect of HIFU on binding site with higher DScore representing better site exposure on MMPs. Conclusion: Multiscale mapping of dentin collagen after HIFU treatment showed no deleterious alterations on the organic structure of dentin.
Original language | English |
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Pages (from-to) | 5344 - 5358 |
Number of pages | 15 |
Journal | Biomaterials Science |
Volume | 9 |
Issue number | 15 |
DOIs | |
Publication status | Published - 7 Aug 2021 |
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Dive into the research topics of 'Characterization of Multiscale Interactions Between High Intensity Focused Ultrasound (HIFU) and Tooth Dentin: Effect on Matrix-Metalloproteinases, Bacterial Biofilms and Biological Properties'. Together they form a unique fingerprint.Projects
- 1 Finished
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Potential of High Intensity Focused Ultrasound (HIFU) in Dental Restorative Fillings: A Novel Approach of Dentin Surface Conditioning
Fawzy Moussa, A. (Investigator 01), Saunders, M. (Investigator 02), Clode, P. (Investigator 03), Baker, M. (Investigator 04) & Kujan, O. (Investigator 05)
NHMRC National Health and Medical Research Council
1/01/20 → 31/12/24
Project: Research