Characterization of Leucetta prolifera, a calcarean cyanosponge from south-western Australia, and its symbionts

J. Fromont, M. J. Huggett, S. K. Lengger, K. Grice, Christine Schonberg

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Abstract

The biology and ecology of calcarean sponges are not as well understood as they are for demosponges. Here, in order to gain new insights, particularly about symbiotic relationships, the calcarean sponge Leucetta prolifera was sampled from southwestern Australia and examined for its assumed photosymbionts. Pulse amplitude modulated fluorometry and extraction of photopigments established that the sponge was photosynthetic. Molecular analysis of the bacterial symbionts via sequencing of the V1 V3 region of the 16S rDNA gene confirmed that between 5 and 22% of all sequences belonged to the phylum Cyanobacteria, depending on the individual sample, with the most dominant strain aligning with Hormoscilla spongeliae, a widely distributed sponge symbiont. Analysis of fatty acids suggested that the sponge obtains nutrition through photosynthates from its symbionts. The relationship is assumed to be mutualistic, with the sponge receiving dietary support and the cyanobacteria sheltering in the sponge tissues. We list all Calcarea presently known to harbour photosymbionts.
Original languageEnglish
Pages (from-to)541-552
Number of pages11
JournalJournal of the Marine Biological Association of the United Kingdom
Volume96
Issue number2
Early online date8 Sep 2015
DOIs
Publication statusPublished - Mar 2016

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Porifera
symbiont
South Australia
sponge
symbionts
Western Australia
Cyanobacteria
cyanobacterium
fluorometry
molecular analysis
photosynthates
nutrition
harbor
fatty acid
ecology
fatty acids
Biological Sciences
gene
genes

Cite this

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abstract = "The biology and ecology of calcarean sponges are not as well understood as they are for demosponges. Here, in order to gain new insights, particularly about symbiotic relationships, the calcarean sponge Leucetta prolifera was sampled from southwestern Australia and examined for its assumed photosymbionts. Pulse amplitude modulated fluorometry and extraction of photopigments established that the sponge was photosynthetic. Molecular analysis of the bacterial symbionts via sequencing of the V1 V3 region of the 16S rDNA gene confirmed that between 5 and 22{\%} of all sequences belonged to the phylum Cyanobacteria, depending on the individual sample, with the most dominant strain aligning with Hormoscilla spongeliae, a widely distributed sponge symbiont. Analysis of fatty acids suggested that the sponge obtains nutrition through photosynthates from its symbionts. The relationship is assumed to be mutualistic, with the sponge receiving dietary support and the cyanobacteria sheltering in the sponge tissues. We list all Calcarea presently known to harbour photosymbionts.",
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Characterization of Leucetta prolifera, a calcarean cyanosponge from south-western Australia, and its symbionts. / Fromont, J.; Huggett, M. J.; Lengger, S. K.; Grice, K.; Schonberg, Christine.

In: Journal of the Marine Biological Association of the United Kingdom, Vol. 96, No. 2, 03.2016, p. 541-552.

Research output: Contribution to journalArticle

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