Characterization of drought tolerance in bread wheat (Triticum aestivum l.) using genetic and transcriptomic tools

Sultan Mia

Research output: Thesis › Doctoral Thesis

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Abstract

The research aimed at understanding the mechanism of drought tolerance in wheat at earty seedling and post-anthesis stages. Under early-stage water stress, contrasting genotypes were identified from a germplasm collection using a stress tolerance index. Transcriptomic analysis of two contrasting genotypes, ABURA and AUS12671, revealed their molecular mechanism of adaptation. For later stage water stress, nature of gene action of a diallel population were analyzed. Finally, C306xDharwar Dry hybrids were used for fast-track development of near-isogenic lines (Nils). These NIL pairs were characterized and can be valuable resources for fine-mapping, and cloning of the gene(s) for drought tolerance in wheat.
Original languageEnglish
QualificationDoctor of Philosophy
Awarding Institution
  • The University of Western Australia
Award date3 Jun 2019
DOIs
Publication statusUnpublished - 2019

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transcriptomics
drought tolerance
water stress
Triticum aestivum
wheat
genotype
germplasm conservation
isogenic lines
stress tolerance
chromosome mapping
molecular cloning
flowering
seedlings
genes

Cite this

Mia, Sultan. / Characterization of drought tolerance in bread wheat (Triticum aestivum l.) using genetic and transcriptomic tools. 2019.
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abstract = "The research aimed at understanding the mechanism of drought tolerance in wheat at earty seedling and post-anthesis stages. Under early-stage water stress, contrasting genotypes were identified from a germplasm collection using a stress tolerance index. Transcriptomic analysis of two contrasting genotypes, ABURA and AUS12671, revealed their molecular mechanism of adaptation. For later stage water stress, nature of gene action of a diallel population were analyzed. Finally, C306xDharwar Dry hybrids were used for fast-track development of near-isogenic lines (Nils). These NIL pairs were characterized and can be valuable resources for fine-mapping, and cloning of the gene(s) for drought tolerance in wheat.",
keywords = "Wheat, Drought tolerance, RNA-seq, Gene expression, Transcriptomics, post-anthesis water stress, root traits, Grain yield",
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school = "The University of Western Australia",

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Mia, S 2019, 'Characterization of drought tolerance in bread wheat (Triticum aestivum l.) using genetic and transcriptomic tools', Doctor of Philosophy, The University of Western Australia. https://doi.org/10.26182/5d26a133a4b9a

Characterization of drought tolerance in bread wheat (Triticum aestivum l.) using genetic and transcriptomic tools. / Mia, Sultan.

2019.

Research output: Thesis › Doctoral Thesis

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T1 - Characterization of drought tolerance in bread wheat (Triticum aestivum l.) using genetic and transcriptomic tools

AU - Mia, Sultan

PY - 2019

Y1 - 2019

N2 - The research aimed at understanding the mechanism of drought tolerance in wheat at earty seedling and post-anthesis stages. Under early-stage water stress, contrasting genotypes were identified from a germplasm collection using a stress tolerance index. Transcriptomic analysis of two contrasting genotypes, ABURA and AUS12671, revealed their molecular mechanism of adaptation. For later stage water stress, nature of gene action of a diallel population were analyzed. Finally, C306xDharwar Dry hybrids were used for fast-track development of near-isogenic lines (Nils). These NIL pairs were characterized and can be valuable resources for fine-mapping, and cloning of the gene(s) for drought tolerance in wheat.

AB - The research aimed at understanding the mechanism of drought tolerance in wheat at earty seedling and post-anthesis stages. Under early-stage water stress, contrasting genotypes were identified from a germplasm collection using a stress tolerance index. Transcriptomic analysis of two contrasting genotypes, ABURA and AUS12671, revealed their molecular mechanism of adaptation. For later stage water stress, nature of gene action of a diallel population were analyzed. Finally, C306xDharwar Dry hybrids were used for fast-track development of near-isogenic lines (Nils). These NIL pairs were characterized and can be valuable resources for fine-mapping, and cloning of the gene(s) for drought tolerance in wheat.

KW - Wheat

KW - Drought tolerance

KW - RNA-seq

KW - Gene expression

KW - Transcriptomics

KW - post-anthesis water stress

KW - root traits

KW - Grain yield

U2 - 10.26182/5d26a133a4b9a

DO - 10.26182/5d26a133a4b9a

M3 - Doctoral Thesis

ER -

Mia S. Characterization of drought tolerance in bread wheat (Triticum aestivum l.) using genetic and transcriptomic tools. 2019. https://doi.org/10.26182/5d26a133a4b9a

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  • THESIS - DOCTOR OF PHILOSOPHY - MIA Md Sultan - 2019

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