Characterization of drought tolerance in bread wheat (Triticum aestivum l.) using genetic and transcriptomic tools

Sultan Mia

doi:10.26182/5d26a133a4b9a

Characterization of drought tolerance in bread wheat (Triticum aestivum l.) using genetic and transcriptomic tools

Sultan Mia

UWA School of Agriculture and Environment

Research output: Thesis › Doctoral Thesis

199 Downloads (Pure)

Abstract

The research aimed at understanding the mechanism of drought tolerance in wheat at earty seedling and post-anthesis stages. Under early-stage water stress, contrasting genotypes were identified from a germplasm collection using a stress tolerance index. Transcriptomic analysis of two contrasting genotypes, ABURA and AUS12671, revealed their molecular mechanism of adaptation. For later stage water stress, nature of gene action of a diallel population were analyzed. Finally, C306xDharwar Dry hybrids were used for fast-track development of near-isogenic lines (Nils). These NIL pairs were characterized and can be valuable resources for fine-mapping, and cloning of the gene(s) for drought tolerance in wheat.

Original language	English
Qualification	Doctor of Philosophy
Awarding Institution	The University of Western Australia
Award date	3 Jun 2019
DOIs	https://doi.org/10.26182/5d26a133a4b9a
Publication status	Unpublished - 2019

Access to Document

10.26182/5d26a133a4b9a

THESIS - DOCTOR OF PHILOSOPHY - MIA Md Sultan - 2019
This work is protected by Copyright. You may print or download ONE copy of this document for the purpose of your own non-commercial research or study. Any other use requires permission from the copyright owner. The Copyright Act requires you to attribute any copyright works you quote or paraphrase.

Fingerprint Dive into the research topics of 'Characterization of drought tolerance in bread wheat (Triticum aestivum l.) using genetic and transcriptomic tools'. Together they form a unique fingerprint.

Cite this

@phdthesis{9a12e4cbfe4b45c2970e2b85595ab502,

title = "Characterization of drought tolerance in bread wheat (Triticum aestivum l.) using genetic and transcriptomic tools",

abstract = "The research aimed at understanding the mechanism of drought tolerance in wheat at earty seedling and post-anthesis stages. Under early-stage water stress, contrasting genotypes were identified from a germplasm collection using a stress tolerance index. Transcriptomic analysis of two contrasting genotypes, ABURA and AUS12671, revealed their molecular mechanism of adaptation. For later stage water stress, nature of gene action of a diallel population were analyzed. Finally, C306xDharwar Dry hybrids were used for fast-track development of near-isogenic lines (Nils). These NIL pairs were characterized and can be valuable resources for fine-mapping, and cloning of the gene(s) for drought tolerance in wheat. ",

keywords = "Wheat, Drought tolerance, RNA-seq, Gene expression, Transcriptomics, post-anthesis water stress, root traits, Grain yield",

author = "Sultan Mia",

year = "2019",

doi = "10.26182/5d26a133a4b9a",

language = "English",

school = "The University of Western Australia",

}

TY - THES

T1 - Characterization of drought tolerance in bread wheat (Triticum aestivum l.) using genetic and transcriptomic tools

AU - Mia, Sultan

PY - 2019

Y1 - 2019

N2 - The research aimed at understanding the mechanism of drought tolerance in wheat at earty seedling and post-anthesis stages. Under early-stage water stress, contrasting genotypes were identified from a germplasm collection using a stress tolerance index. Transcriptomic analysis of two contrasting genotypes, ABURA and AUS12671, revealed their molecular mechanism of adaptation. For later stage water stress, nature of gene action of a diallel population were analyzed. Finally, C306xDharwar Dry hybrids were used for fast-track development of near-isogenic lines (Nils). These NIL pairs were characterized and can be valuable resources for fine-mapping, and cloning of the gene(s) for drought tolerance in wheat.

AB - The research aimed at understanding the mechanism of drought tolerance in wheat at earty seedling and post-anthesis stages. Under early-stage water stress, contrasting genotypes were identified from a germplasm collection using a stress tolerance index. Transcriptomic analysis of two contrasting genotypes, ABURA and AUS12671, revealed their molecular mechanism of adaptation. For later stage water stress, nature of gene action of a diallel population were analyzed. Finally, C306xDharwar Dry hybrids were used for fast-track development of near-isogenic lines (Nils). These NIL pairs were characterized and can be valuable resources for fine-mapping, and cloning of the gene(s) for drought tolerance in wheat.

KW - Wheat

KW - Drought tolerance

KW - RNA-seq

KW - Gene expression

KW - Transcriptomics

KW - post-anthesis water stress

KW - root traits

KW - Grain yield

U2 - 10.26182/5d26a133a4b9a

DO - 10.26182/5d26a133a4b9a

M3 - Doctoral Thesis

ER -