Characterization of drought tolerance in bread wheat (Triticum aestivum l.) using genetic and transcriptomic tools

Sultan Mia

Research output: ThesisDoctoral Thesis

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Abstract

The research aimed at understanding the mechanism of drought tolerance in wheat at earty seedling and post-anthesis stages. Under early-stage water stress, contrasting genotypes were identified from a germplasm collection using a stress tolerance index. Transcriptomic analysis of two contrasting genotypes, ABURA and AUS12671, revealed their molecular mechanism of adaptation. For later stage water stress, nature of gene action of a diallel population were analyzed. Finally, C306xDharwar Dry hybrids were used for fast-track development of near-isogenic lines (Nils). These NIL pairs were characterized and can be valuable resources for fine-mapping, and cloning of the gene(s) for drought tolerance in wheat.
Original languageEnglish
QualificationDoctor of Philosophy
Awarding Institution
  • The University of Western Australia
Award date3 Jun 2019
DOIs
Publication statusUnpublished - 2019

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transcriptomics
drought tolerance
water stress
Triticum aestivum
wheat
genotype
germplasm conservation
isogenic lines
stress tolerance
chromosome mapping
molecular cloning
flowering
seedlings
genes

Cite this

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title = "Characterization of drought tolerance in bread wheat (Triticum aestivum l.) using genetic and transcriptomic tools",
abstract = "The research aimed at understanding the mechanism of drought tolerance in wheat at earty seedling and post-anthesis stages. Under early-stage water stress, contrasting genotypes were identified from a germplasm collection using a stress tolerance index. Transcriptomic analysis of two contrasting genotypes, ABURA and AUS12671, revealed their molecular mechanism of adaptation. For later stage water stress, nature of gene action of a diallel population were analyzed. Finally, C306xDharwar Dry hybrids were used for fast-track development of near-isogenic lines (Nils). These NIL pairs were characterized and can be valuable resources for fine-mapping, and cloning of the gene(s) for drought tolerance in wheat.",
keywords = "Wheat, Drought tolerance, RNA-seq, Gene expression, Transcriptomics, post-anthesis water stress, root traits, Grain yield",
author = "Sultan Mia",
year = "2019",
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language = "English",
school = "The University of Western Australia",

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N2 - The research aimed at understanding the mechanism of drought tolerance in wheat at earty seedling and post-anthesis stages. Under early-stage water stress, contrasting genotypes were identified from a germplasm collection using a stress tolerance index. Transcriptomic analysis of two contrasting genotypes, ABURA and AUS12671, revealed their molecular mechanism of adaptation. For later stage water stress, nature of gene action of a diallel population were analyzed. Finally, C306xDharwar Dry hybrids were used for fast-track development of near-isogenic lines (Nils). These NIL pairs were characterized and can be valuable resources for fine-mapping, and cloning of the gene(s) for drought tolerance in wheat.

AB - The research aimed at understanding the mechanism of drought tolerance in wheat at earty seedling and post-anthesis stages. Under early-stage water stress, contrasting genotypes were identified from a germplasm collection using a stress tolerance index. Transcriptomic analysis of two contrasting genotypes, ABURA and AUS12671, revealed their molecular mechanism of adaptation. For later stage water stress, nature of gene action of a diallel population were analyzed. Finally, C306xDharwar Dry hybrids were used for fast-track development of near-isogenic lines (Nils). These NIL pairs were characterized and can be valuable resources for fine-mapping, and cloning of the gene(s) for drought tolerance in wheat.

KW - Wheat

KW - Drought tolerance

KW - RNA-seq

KW - Gene expression

KW - Transcriptomics

KW - post-anthesis water stress

KW - root traits

KW - Grain yield

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M3 - Doctoral Thesis

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