Characterization and Transferability of Microsatellites for the Kangaroo Paw, Anigozanthos manglesii (Haemodoraceae)

Bronwyn M. Ayre; Janet M. Anthony; David G. Roberts; Richard J. N. Allcock; Siegfried L. Krauss

doi:10.3732/apps.1700055

Characterization and Transferability of Microsatellites for the Kangaroo Paw, Anigozanthos manglesii (Haemodoraceae)

Bronwyn M. Ayre, Janet M. Anthony, David G. Roberts, Richard J. N. Allcock, Siegfried L. Krauss

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

Premise of the study: Microsatellites were developed for the future assessment of population genetic structure, mating system, and dispersal of the perennial kangaroo paw, Anigozanthos manglesii (Haemodoraceae), and related species.

Methods and Results: Using a Personal Genome Machine (PGM) semiconductor sequencer, ca. 4.03 million sequence reads were generated. QDD pipeline software was used to identify 190,000 microsatellite-containing regions and priming sites. From these, 90 were chosen and screened using PCR, and 15 polymorphic markers identified. These sites amplified di-, tri-, and pentanucleotide repeats with one to 20 alleles per locus. Primers were also amplified across congeners A. bicolor, A. flavidus, A. gabrielae, A. humilis, A. preissii, A. pulcherrimus, A. rufus, and A. viridis to assess cross-species transferability.

Conclusions: These markers provide a resource for population genetic studies in A. manglesii and other species within the genus.

Original language	English
Article number	1700055
Number of pages	4
Journal	Applications in Plant Sciences
Volume	5
Issue number	8
DOIs	https://doi.org/10.3732/apps.1700055
Publication status	Published - Aug 2017

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10.3732/apps.1700055Licence: CC BY-NC-SA

1 Finished

The evolution and conservation consequences of promiscuity in plants pollinated by vertebrates
Hopper, S. (Investigator 01), Krauss, S. (Investigator 02) & Phillips, R. (Investigator 03)
ARC Australian Research Council
1/01/14 → 31/12/17
Project: Research

5 Citations
1 Doctoral Thesis

The critical role of birds as pollinators of the Red and Green Kangaroo Paw, Anigozanthos manglesii
Ayre, B., 2020, (Unpublished)
Research output: Thesis › Doctoral Thesis

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@article{367e9791ec8849649444cf38b2167d5c,

title = "Characterization and Transferability of Microsatellites for the Kangaroo Paw, Anigozanthos manglesii (Haemodoraceae)",

abstract = "Premise of the study: Microsatellites were developed for the future assessment of population genetic structure, mating system, and dispersal of the perennial kangaroo paw, Anigozanthos manglesii (Haemodoraceae), and related species.Methods and Results: Using a Personal Genome Machine (PGM) semiconductor sequencer, ca. 4.03 million sequence reads were generated. QDD pipeline software was used to identify 190,000 microsatellite-containing regions and priming sites. From these, 90 were chosen and screened using PCR, and 15 polymorphic markers identified. These sites amplified di-, tri-, and pentanucleotide repeats with one to 20 alleles per locus. Primers were also amplified across congeners A. bicolor, A. flavidus, A. gabrielae, A. humilis, A. preissii, A. pulcherrimus, A. rufus, and A. viridis to assess cross-species transferability.Conclusions: These markers provide a resource for population genetic studies in A. manglesii and other species within the genus.",

keywords = "Anigozanthos, Catspaw, Haemodoraceae, Kangaroo Paw, microsatellite primers, SOFTWARE",

author = "Ayre, {Bronwyn M.} and Anthony, {Janet M.} and Roberts, {David G.} and Allcock, {Richard J. N.} and Krauss, {Siegfried L.}",

year = "2017",

month = aug,

doi = "10.3732/apps.1700055",

language = "English",

volume = "5",

journal = "Applications in Plant Sciences",

issn = "2168-0450",

publisher = "John Wiley & Sons",

number = "8",

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T1 - Characterization and Transferability of Microsatellites for the Kangaroo Paw, Anigozanthos manglesii (Haemodoraceae)

AU - Ayre, Bronwyn M.

AU - Anthony, Janet M.

AU - Roberts, David G.

AU - Allcock, Richard J. N.

AU - Krauss, Siegfried L.

PY - 2017/8

Y1 - 2017/8

N2 - Premise of the study: Microsatellites were developed for the future assessment of population genetic structure, mating system, and dispersal of the perennial kangaroo paw, Anigozanthos manglesii (Haemodoraceae), and related species.Methods and Results: Using a Personal Genome Machine (PGM) semiconductor sequencer, ca. 4.03 million sequence reads were generated. QDD pipeline software was used to identify 190,000 microsatellite-containing regions and priming sites. From these, 90 were chosen and screened using PCR, and 15 polymorphic markers identified. These sites amplified di-, tri-, and pentanucleotide repeats with one to 20 alleles per locus. Primers were also amplified across congeners A. bicolor, A. flavidus, A. gabrielae, A. humilis, A. preissii, A. pulcherrimus, A. rufus, and A. viridis to assess cross-species transferability.Conclusions: These markers provide a resource for population genetic studies in A. manglesii and other species within the genus.

AB - Premise of the study: Microsatellites were developed for the future assessment of population genetic structure, mating system, and dispersal of the perennial kangaroo paw, Anigozanthos manglesii (Haemodoraceae), and related species.Methods and Results: Using a Personal Genome Machine (PGM) semiconductor sequencer, ca. 4.03 million sequence reads were generated. QDD pipeline software was used to identify 190,000 microsatellite-containing regions and priming sites. From these, 90 were chosen and screened using PCR, and 15 polymorphic markers identified. These sites amplified di-, tri-, and pentanucleotide repeats with one to 20 alleles per locus. Primers were also amplified across congeners A. bicolor, A. flavidus, A. gabrielae, A. humilis, A. preissii, A. pulcherrimus, A. rufus, and A. viridis to assess cross-species transferability.Conclusions: These markers provide a resource for population genetic studies in A. manglesii and other species within the genus.

KW - Anigozanthos

KW - Catspaw

KW - Haemodoraceae

KW - Kangaroo Paw

KW - microsatellite primers

KW - SOFTWARE

U2 - 10.3732/apps.1700055

DO - 10.3732/apps.1700055

M3 - Article

C2 - 28924516

SN - 2168-0450

VL - 5

JO - Applications in Plant Sciences

JF - Applications in Plant Sciences

IS - 8

M1 - 1700055

ER -

Characterization and Transferability of Microsatellites for the Kangaroo Paw, Anigozanthos manglesii (Haemodoraceae)

Abstract

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The evolution and conservation consequences of promiscuity in plants pollinated by vertebrates

The critical role of birds as pollinators of the Red and Green Kangaroo Paw, Anigozanthos manglesii

Cite this

Characterization and Transferability of Microsatellites for the Kangaroo Paw, Anigozanthos manglesii (Haemodoraceae)

Abstract

Access to Document

Fingerprint

Projects

The evolution and conservation consequences of promiscuity in plants pollinated by vertebrates

Research output

The critical role of birds as pollinators of the Red and Green Kangaroo Paw, Anigozanthos manglesii

Cite this