Characterization and Transferability of Microsatellites for the Kangaroo Paw, Anigozanthos manglesii (Haemodoraceae)

Bronwyn M. Ayre, Janet M. Anthony, David G. Roberts, Richard J. N. Allcock, Siegfried L. Krauss

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2 Citations (Scopus)

Abstract

Premise of the study: Microsatellites were developed for the future assessment of population genetic structure, mating system, and dispersal of the perennial kangaroo paw, Anigozanthos manglesii (Haemodoraceae), and related species.

Methods and Results: Using a Personal Genome Machine (PGM) semiconductor sequencer, ca. 4.03 million sequence reads were generated. QDD pipeline software was used to identify 190,000 microsatellite-containing regions and priming sites. From these, 90 were chosen and screened using PCR, and 15 polymorphic markers identified. These sites amplified di-, tri-, and pentanucleotide repeats with one to 20 alleles per locus. Primers were also amplified across congeners A. bicolor, A. flavidus, A. gabrielae, A. humilis, A. preissii, A. pulcherrimus, A. rufus, and A. viridis to assess cross-species transferability.

Conclusions: These markers provide a resource for population genetic studies in A. manglesii and other species within the genus.

Original languageEnglish
Article number1700055
Number of pages4
JournalApplications in Plant Sciences
Volume5
Issue number8
DOIs
Publication statusPublished - Aug 2017

Cite this

@article{367e9791ec8849649444cf38b2167d5c,
title = "Characterization and Transferability of Microsatellites for the Kangaroo Paw, Anigozanthos manglesii (Haemodoraceae)",
abstract = "Premise of the study: Microsatellites were developed for the future assessment of population genetic structure, mating system, and dispersal of the perennial kangaroo paw, Anigozanthos manglesii (Haemodoraceae), and related species.Methods and Results: Using a Personal Genome Machine (PGM) semiconductor sequencer, ca. 4.03 million sequence reads were generated. QDD pipeline software was used to identify 190,000 microsatellite-containing regions and priming sites. From these, 90 were chosen and screened using PCR, and 15 polymorphic markers identified. These sites amplified di-, tri-, and pentanucleotide repeats with one to 20 alleles per locus. Primers were also amplified across congeners A. bicolor, A. flavidus, A. gabrielae, A. humilis, A. preissii, A. pulcherrimus, A. rufus, and A. viridis to assess cross-species transferability.Conclusions: These markers provide a resource for population genetic studies in A. manglesii and other species within the genus.",
keywords = "Anigozanthos, Catspaw, Haemodoraceae, Kangaroo Paw, microsatellite primers, SOFTWARE",
author = "Ayre, {Bronwyn M.} and Anthony, {Janet M.} and Roberts, {David G.} and Allcock, {Richard J. N.} and Krauss, {Siegfried L.}",
year = "2017",
month = "8",
doi = "10.3732/apps.1700055",
language = "English",
volume = "5",
journal = "Applications in Plant Sciences",
issn = "2168-0450",
publisher = "John Wiley & Sons",
number = "8",

}

TY - JOUR

T1 - Characterization and Transferability of Microsatellites for the Kangaroo Paw, Anigozanthos manglesii (Haemodoraceae)

AU - Ayre, Bronwyn M.

AU - Anthony, Janet M.

AU - Roberts, David G.

AU - Allcock, Richard J. N.

AU - Krauss, Siegfried L.

PY - 2017/8

Y1 - 2017/8

N2 - Premise of the study: Microsatellites were developed for the future assessment of population genetic structure, mating system, and dispersal of the perennial kangaroo paw, Anigozanthos manglesii (Haemodoraceae), and related species.Methods and Results: Using a Personal Genome Machine (PGM) semiconductor sequencer, ca. 4.03 million sequence reads were generated. QDD pipeline software was used to identify 190,000 microsatellite-containing regions and priming sites. From these, 90 were chosen and screened using PCR, and 15 polymorphic markers identified. These sites amplified di-, tri-, and pentanucleotide repeats with one to 20 alleles per locus. Primers were also amplified across congeners A. bicolor, A. flavidus, A. gabrielae, A. humilis, A. preissii, A. pulcherrimus, A. rufus, and A. viridis to assess cross-species transferability.Conclusions: These markers provide a resource for population genetic studies in A. manglesii and other species within the genus.

AB - Premise of the study: Microsatellites were developed for the future assessment of population genetic structure, mating system, and dispersal of the perennial kangaroo paw, Anigozanthos manglesii (Haemodoraceae), and related species.Methods and Results: Using a Personal Genome Machine (PGM) semiconductor sequencer, ca. 4.03 million sequence reads were generated. QDD pipeline software was used to identify 190,000 microsatellite-containing regions and priming sites. From these, 90 were chosen and screened using PCR, and 15 polymorphic markers identified. These sites amplified di-, tri-, and pentanucleotide repeats with one to 20 alleles per locus. Primers were also amplified across congeners A. bicolor, A. flavidus, A. gabrielae, A. humilis, A. preissii, A. pulcherrimus, A. rufus, and A. viridis to assess cross-species transferability.Conclusions: These markers provide a resource for population genetic studies in A. manglesii and other species within the genus.

KW - Anigozanthos

KW - Catspaw

KW - Haemodoraceae

KW - Kangaroo Paw

KW - microsatellite primers

KW - SOFTWARE

U2 - 10.3732/apps.1700055

DO - 10.3732/apps.1700055

M3 - Article

VL - 5

JO - Applications in Plant Sciences

JF - Applications in Plant Sciences

SN - 2168-0450

IS - 8

M1 - 1700055

ER -