TY - JOUR
T1 - Characterization and mapping of LanrBo: a locus conferring anthracnose resistance in narrow-leafed lupin (Lupinus angustifolius L.)
AU - Fischer, K.
AU - Dieterich, R.
AU - Nelson, Matthew
AU - Kamphuis, Lars
AU - Singh, Karam
AU - Rotter, B.
AU - Krezdorn, N.
AU - Winter, P.
AU - Wehling, P.
AU - Ruge-Wehling, B.
PY - 2015
Y1 - 2015
N2 - © 2015, Springer-Verlag Berlin Heidelberg. Key message: A novel and highly effective source of anthracnose resistance in narrow-leafed lupin was identified. Resistance was shown to be governed by a single dominant locus. Molecular markers have been developed, which can be used for selecting resistant genotypes in lupin breeding. Abstract: A screening for anthracnose resistance of a set of plant genetic resources of narrow-leafed lupin (Lupinus angustifolius L.) identified the breeding line Bo7212 as being highly resistant to anthracnose (Colletotrichum lupini). Segregation analysis indicated that the resistance of Bo7212 is inherited by a single dominant locus. The corresponding resistance gene was given the designation LanrBo. Previously published molecular anchor markers allowed us to locate LanrBo on linkage group NLL-11 of narrow-leafed lupin. Using information from RNAseq data obtained with inoculated resistant vs. susceptible lupin entries as well as EST-sequence information from the model genome Lotus japonicus, additional SNP and EST markers linked to LanrBo were derived. A bracket of two LanrBo-flanking markers allows for precise marker-assisted selection of the novel resistance gene in narrow-leafed lupin breeding programs.
AB - © 2015, Springer-Verlag Berlin Heidelberg. Key message: A novel and highly effective source of anthracnose resistance in narrow-leafed lupin was identified. Resistance was shown to be governed by a single dominant locus. Molecular markers have been developed, which can be used for selecting resistant genotypes in lupin breeding. Abstract: A screening for anthracnose resistance of a set of plant genetic resources of narrow-leafed lupin (Lupinus angustifolius L.) identified the breeding line Bo7212 as being highly resistant to anthracnose (Colletotrichum lupini). Segregation analysis indicated that the resistance of Bo7212 is inherited by a single dominant locus. The corresponding resistance gene was given the designation LanrBo. Previously published molecular anchor markers allowed us to locate LanrBo on linkage group NLL-11 of narrow-leafed lupin. Using information from RNAseq data obtained with inoculated resistant vs. susceptible lupin entries as well as EST-sequence information from the model genome Lotus japonicus, additional SNP and EST markers linked to LanrBo were derived. A bracket of two LanrBo-flanking markers allows for precise marker-assisted selection of the novel resistance gene in narrow-leafed lupin breeding programs.
U2 - 10.1007/s00122-015-2572-3
DO - 10.1007/s00122-015-2572-3
M3 - Article
C2 - 26169875
VL - 128
SP - 2121
EP - 2130
JO - TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik
JF - TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik
SN - 0040-5752
IS - 10
ER -