Characteristics of membrane-bound fatty acid desaturase (FAD) genes in Brassica napus L. and their expressions under different cadmium and salinity stresses

Ling Xu, Wujing Zeng, Juanjuan Li, Hui Liu, Guijun Yan, Ping Si, Chong Yang, Ying Shi, Qiuling He, Weijun Zhou

Research output: Contribution to journalArticle

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Abstract

The membrane-bound fatty acid desaturases (FADs) play vital roles in regulating plant fatty acid (FA) compositions which directly affect oil quality in rapeseed (Brassica napus L.). In the present study, a total of 68 putative FAD genes were identified in the crop and were phylogenetically clustered into four subfamilies. Genomic localization revealed that the genes were distributed across 21 chromosomes at various densities. The structures of paralogous genes were considerably conserved in the number of exons and in the length of introns. It was found that the expansion of the B. napus FAD family was mainly attributable to whole genome duplication and segmental duplication. Rapeseed seedlings were grown hydroponically under three levels (0, 250, and 500 μM) of cadmium (Cd) and three levels (0, 50, and 100 mM) of salinity. After one week of treatment, the roots and leaves of seedlings were harvested to study the expression patterns of 68 selected BnFADs in response to the different stresses. The results from qRT-PCR analyses suggested that the FAD genes in rapeseed were differentially expressed under various abiotic stresses. The transcript levels of the FAD genes ADS4.8, ADS4.9, ADS4.10, ADS9.1 and SLD8 in the leaves of Cd-tolerant cultivar ZD619 were significantly higher than those in the leaves of sensitive cultivar ZD622 under the 250 μM Cd stress condition. Moreover, the expression levels of 8FAD genes (ADS4.1, ADS4.4, ADS4.9, ADS9.1, ADS9.2, SLD8, FAD7.4 and FAD7.5) in ZD619 roots were obviously enhanced with the increase of salinity concentrations. These results indicate the likelihood of FAD genes involved in Cd and salinity tolerance. This study provides insight on membrane-bound FAD genes involvement in tolerance to abiotic stresses and would provide some more candidate genes for the crop improvement.

Original languageEnglish
Pages (from-to)144-156
Number of pages13
JournalEnvironmental and Experimental Botany
Volume162
DOIs
Publication statusPublished - 1 Jun 2019

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stearoyl-CoA desaturase
Brassica napus
salt stress
cadmium
fatty acid
membrane
salinity
gene
genes
rapeseed
abiotic stress
cultivar
seedling
crop improvement
leaves
salinity tolerance
seedlings
cultivars
crops
exons

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title = "Characteristics of membrane-bound fatty acid desaturase (FAD) genes in Brassica napus L. and their expressions under different cadmium and salinity stresses",
abstract = "The membrane-bound fatty acid desaturases (FADs) play vital roles in regulating plant fatty acid (FA) compositions which directly affect oil quality in rapeseed (Brassica napus L.). In the present study, a total of 68 putative FAD genes were identified in the crop and were phylogenetically clustered into four subfamilies. Genomic localization revealed that the genes were distributed across 21 chromosomes at various densities. The structures of paralogous genes were considerably conserved in the number of exons and in the length of introns. It was found that the expansion of the B. napus FAD family was mainly attributable to whole genome duplication and segmental duplication. Rapeseed seedlings were grown hydroponically under three levels (0, 250, and 500 μM) of cadmium (Cd) and three levels (0, 50, and 100 mM) of salinity. After one week of treatment, the roots and leaves of seedlings were harvested to study the expression patterns of 68 selected BnFADs in response to the different stresses. The results from qRT-PCR analyses suggested that the FAD genes in rapeseed were differentially expressed under various abiotic stresses. The transcript levels of the FAD genes ADS4.8, ADS4.9, ADS4.10, ADS9.1 and SLD8 in the leaves of Cd-tolerant cultivar ZD619 were significantly higher than those in the leaves of sensitive cultivar ZD622 under the 250 μM Cd stress condition. Moreover, the expression levels of 8FAD genes (ADS4.1, ADS4.4, ADS4.9, ADS9.1, ADS9.2, SLD8, FAD7.4 and FAD7.5) in ZD619 roots were obviously enhanced with the increase of salinity concentrations. These results indicate the likelihood of FAD genes involved in Cd and salinity tolerance. This study provides insight on membrane-bound FAD genes involvement in tolerance to abiotic stresses and would provide some more candidate genes for the crop improvement.",
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Characteristics of membrane-bound fatty acid desaturase (FAD) genes in Brassica napus L. and their expressions under different cadmium and salinity stresses. / Xu, Ling; Zeng, Wujing; Li, Juanjuan; Liu, Hui; Yan, Guijun; Si, Ping; Yang, Chong; Shi, Ying; He, Qiuling; Zhou, Weijun.

In: Environmental and Experimental Botany, Vol. 162, 01.06.2019, p. 144-156.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Characteristics of membrane-bound fatty acid desaturase (FAD) genes in Brassica napus L. and their expressions under different cadmium and salinity stresses

AU - Xu, Ling

AU - Zeng, Wujing

AU - Li, Juanjuan

AU - Liu, Hui

AU - Yan, Guijun

AU - Si, Ping

AU - Yang, Chong

AU - Shi, Ying

AU - He, Qiuling

AU - Zhou, Weijun

PY - 2019/6/1

Y1 - 2019/6/1

N2 - The membrane-bound fatty acid desaturases (FADs) play vital roles in regulating plant fatty acid (FA) compositions which directly affect oil quality in rapeseed (Brassica napus L.). In the present study, a total of 68 putative FAD genes were identified in the crop and were phylogenetically clustered into four subfamilies. Genomic localization revealed that the genes were distributed across 21 chromosomes at various densities. The structures of paralogous genes were considerably conserved in the number of exons and in the length of introns. It was found that the expansion of the B. napus FAD family was mainly attributable to whole genome duplication and segmental duplication. Rapeseed seedlings were grown hydroponically under three levels (0, 250, and 500 μM) of cadmium (Cd) and three levels (0, 50, and 100 mM) of salinity. After one week of treatment, the roots and leaves of seedlings were harvested to study the expression patterns of 68 selected BnFADs in response to the different stresses. The results from qRT-PCR analyses suggested that the FAD genes in rapeseed were differentially expressed under various abiotic stresses. The transcript levels of the FAD genes ADS4.8, ADS4.9, ADS4.10, ADS9.1 and SLD8 in the leaves of Cd-tolerant cultivar ZD619 were significantly higher than those in the leaves of sensitive cultivar ZD622 under the 250 μM Cd stress condition. Moreover, the expression levels of 8FAD genes (ADS4.1, ADS4.4, ADS4.9, ADS9.1, ADS9.2, SLD8, FAD7.4 and FAD7.5) in ZD619 roots were obviously enhanced with the increase of salinity concentrations. These results indicate the likelihood of FAD genes involved in Cd and salinity tolerance. This study provides insight on membrane-bound FAD genes involvement in tolerance to abiotic stresses and would provide some more candidate genes for the crop improvement.

AB - The membrane-bound fatty acid desaturases (FADs) play vital roles in regulating plant fatty acid (FA) compositions which directly affect oil quality in rapeseed (Brassica napus L.). In the present study, a total of 68 putative FAD genes were identified in the crop and were phylogenetically clustered into four subfamilies. Genomic localization revealed that the genes were distributed across 21 chromosomes at various densities. The structures of paralogous genes were considerably conserved in the number of exons and in the length of introns. It was found that the expansion of the B. napus FAD family was mainly attributable to whole genome duplication and segmental duplication. Rapeseed seedlings were grown hydroponically under three levels (0, 250, and 500 μM) of cadmium (Cd) and three levels (0, 50, and 100 mM) of salinity. After one week of treatment, the roots and leaves of seedlings were harvested to study the expression patterns of 68 selected BnFADs in response to the different stresses. The results from qRT-PCR analyses suggested that the FAD genes in rapeseed were differentially expressed under various abiotic stresses. The transcript levels of the FAD genes ADS4.8, ADS4.9, ADS4.10, ADS9.1 and SLD8 in the leaves of Cd-tolerant cultivar ZD619 were significantly higher than those in the leaves of sensitive cultivar ZD622 under the 250 μM Cd stress condition. Moreover, the expression levels of 8FAD genes (ADS4.1, ADS4.4, ADS4.9, ADS9.1, ADS9.2, SLD8, FAD7.4 and FAD7.5) in ZD619 roots were obviously enhanced with the increase of salinity concentrations. These results indicate the likelihood of FAD genes involved in Cd and salinity tolerance. This study provides insight on membrane-bound FAD genes involvement in tolerance to abiotic stresses and would provide some more candidate genes for the crop improvement.

KW - Abiotic stresses

KW - Brassica napus L.

KW - Characterization

KW - Evolution

KW - Expression analyses

KW - Membrane-bound fatty acid desaturase genes

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