Characterising the morphology, phylogeny, ecology and distribution of the arbuscular mycorrhizal fungi, fine root endophytes

Suzanne Orchard

Research output: ThesisDoctoral Thesis

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Abstract

Fine root endophytes (FRE; Glomus tenue) are root-endophytic fungi traditionally classified within the arbuscular mycorrhizal fungi (AMF; phylum Glomeromycota). However, their placement within Glomus, and their ecological function, was obscure. I grew Trifolium subterraneum roots enriched with FRE, extracted DNA for lllumina next-generation sequencing, and demonstrated that FRE phylogenetically align with Mucoromycotina-not Glomeromycota .Through a literature meta-analysis, cryo-scanning electron microscopy and a distribution survey I found evidence that FRE play a role in plant nutrient acquisition, but are likely influenced by different edaphic variables than AMF. Therefore, FRE differ from AMF in phylogenetic placement, physiology and ecological niche.
Original languageEnglish
QualificationDoctor of Philosophy
Awarding Institution
  • The University of Western Australia
Thesis sponsors
Award date25 Oct 2017
DOIs
Publication statusUnpublished - 2017

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Glomeromycota
Glomus
endophytes
mycorrhizal fungi
Mucoromycotina
ecology
Trifolium subterraneum
ecological function
phylogeny
meta-analysis
niches
scanning electron microscopy
physiology
fungi
nutrients
DNA
fine roots

Cite this

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title = "Characterising the morphology, phylogeny, ecology and distribution of the arbuscular mycorrhizal fungi, fine root endophytes",
abstract = "Fine root endophytes (FRE; Glomus tenue) are root-endophytic fungi traditionally classified within the arbuscular mycorrhizal fungi (AMF; phylum Glomeromycota). However, their placement within Glomus, and their ecological function, was obscure. I grew Trifolium subterraneum roots enriched with FRE, extracted DNA for lllumina next-generation sequencing, and demonstrated that FRE phylogenetically align with Mucoromycotina-not Glomeromycota .Through a literature meta-analysis, cryo-scanning electron microscopy and a distribution survey I found evidence that FRE play a role in plant nutrient acquisition, but are likely influenced by different edaphic variables than AMF. Therefore, FRE differ from AMF in phylogenetic placement, physiology and ecological niche.",
keywords = "Arbuscular mycorrhizal fungi, fine root endophyte, Glomeromycota, Glomus tenue, Glomus tenuis, Mucoromycotina",
author = "Suzanne Orchard",
year = "2017",
doi = "10.4225/23/59faa02afe7bb",
language = "English",
school = "The University of Western Australia",

}

TY - THES

T1 - Characterising the morphology, phylogeny, ecology and distribution of the arbuscular mycorrhizal fungi, fine root endophytes

AU - Orchard, Suzanne

PY - 2017

Y1 - 2017

N2 - Fine root endophytes (FRE; Glomus tenue) are root-endophytic fungi traditionally classified within the arbuscular mycorrhizal fungi (AMF; phylum Glomeromycota). However, their placement within Glomus, and their ecological function, was obscure. I grew Trifolium subterraneum roots enriched with FRE, extracted DNA for lllumina next-generation sequencing, and demonstrated that FRE phylogenetically align with Mucoromycotina-not Glomeromycota .Through a literature meta-analysis, cryo-scanning electron microscopy and a distribution survey I found evidence that FRE play a role in plant nutrient acquisition, but are likely influenced by different edaphic variables than AMF. Therefore, FRE differ from AMF in phylogenetic placement, physiology and ecological niche.

AB - Fine root endophytes (FRE; Glomus tenue) are root-endophytic fungi traditionally classified within the arbuscular mycorrhizal fungi (AMF; phylum Glomeromycota). However, their placement within Glomus, and their ecological function, was obscure. I grew Trifolium subterraneum roots enriched with FRE, extracted DNA for lllumina next-generation sequencing, and demonstrated that FRE phylogenetically align with Mucoromycotina-not Glomeromycota .Through a literature meta-analysis, cryo-scanning electron microscopy and a distribution survey I found evidence that FRE play a role in plant nutrient acquisition, but are likely influenced by different edaphic variables than AMF. Therefore, FRE differ from AMF in phylogenetic placement, physiology and ecological niche.

KW - Arbuscular mycorrhizal fungi

KW - fine root endophyte

KW - Glomeromycota

KW - Glomus tenue

KW - Glomus tenuis

KW - Mucoromycotina

U2 - 10.4225/23/59faa02afe7bb

DO - 10.4225/23/59faa02afe7bb

M3 - Doctoral Thesis

ER -