Characterisation of polymorphic microsatellite markers in the widespread Australian seagrass, Posidonia australis Hook. f. (Posidoniaceae), with cross-amplification in the sympatric P.sinuosa

Elizabeth Sinclair, J. Anthony, Grey Coupland, M. Waycott, Matthew Barrett, Russell Barrett, Marion Cambridge, Mark Wallace, Kingsley Dixon, Siegfried Krauss, Gary Kendrick

    Research output: Contribution to journalArticle

    15 Citations (Scopus)

    Abstract

    We developed 10 polymorphic microsatellite markers in the Australian seagrass Posidonia australis Hook. f. Markers were screened for their ability to detect within- and among-population genetic structure and variation. The markers showed a range in levels of polymorphism from fixed differences between the two sampled seagrass meadows to high levels of heterozygosity. These markers will be used to estimate gene flow across the species range, characterise the mating system through paternity analysis and pollen dispersal, characterise the nature and extent of clonality, and determine the genetic differentiation of local seagrass meadows to provide information on where to source local genetic provenance material for seagrass restoration projects. Seven of the 10 loci also amplified in the sympatric P. sinuosa and will be useful in future studies in population genetics and hybridisation.
    Original languageEnglish
    Pages (from-to)273-276
    JournalConservation Genetics Resources
    Volume1
    Issue number1
    Publication statusPublished - 2009

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