Characterisation of microsatellite DNA markers for the Wiry Honey Myrtle, Melaleuca nematophylla Craven

Melissa Millar; Margaret Byrne; David Coates; Dale Roberts

doi:10.1007/s12686-013-0118-z

Characterisation of microsatellite DNA markers for the Wiry Honey Myrtle, Melaleuca nematophylla Craven

Melissa Millar, Margaret Byrne, David Coates, Dale Roberts

Centre of Excellence in Natural Resource Management

Research output: Contribution to journal › Letter

1 Citation (Scopus)

Abstract

A genomic library was constructed and 14 novel polymorphic di- and tri-nucleotide nuclear microsatellite markers were developed for Melaleuca nematophylla, the Wiry Honey Myrtle, a common shrub of southwest Western Australia. Populations are patchily distributed in a highly fragmented landscape due to extensive vegetation clearing for agriculture and population genetic structure is being investigated to inform appropriate seed collection and restoration strategies. Moderate diversity was observed in two populations with the number of alleles per locus ranging from 2 to 17. Expected and observed heterozygosity averaged 0.606 and 0.423, respectively. All loci showed independent inheritance but there was evidence of possible null alleles at some loci in each population. © 2014 @Crown copyright as represented by the state of Western Australia.

Original language	English
Pages (from-to)	439-441
Number of pages	3
Journal	Conservation Genetics Resources
Volume	6
Issue number	2
Early online date	11 Jan 2014
DOIs	https://doi.org/10.1007/s12686-013-0118-z
Publication status	Published - Jun 2014

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title = "Characterisation of microsatellite DNA markers for the Wiry Honey Myrtle, Melaleuca nematophylla Craven",

abstract = "A genomic library was constructed and 14 novel polymorphic di- and tri-nucleotide nuclear microsatellite markers were developed for Melaleuca nematophylla, the Wiry Honey Myrtle, a common shrub of southwest Western Australia. Populations are patchily distributed in a highly fragmented landscape due to extensive vegetation clearing for agriculture and population genetic structure is being investigated to inform appropriate seed collection and restoration strategies. Moderate diversity was observed in two populations with the number of alleles per locus ranging from 2 to 17. Expected and observed heterozygosity averaged 0.606 and 0.423, respectively. All loci showed independent inheritance but there was evidence of possible null alleles at some loci in each population. {\textcopyright} 2014 @Crown copyright as represented by the state of Western Australia.",

author = "Melissa Millar and Margaret Byrne and David Coates and Dale Roberts",

year = "2014",

month = jun,

doi = "10.1007/s12686-013-0118-z",

language = "English",

volume = "6",

pages = "439--441",

journal = "Conservation Genetics Resources",

issn = "1877-7252",

publisher = "Springer-Verlag London Ltd.",

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TY - JOUR

T1 - Characterisation of microsatellite DNA markers for the Wiry Honey Myrtle, Melaleuca nematophylla Craven

AU - Millar, Melissa

AU - Byrne, Margaret

AU - Coates, David

AU - Roberts, Dale

PY - 2014/6

Y1 - 2014/6

N2 - A genomic library was constructed and 14 novel polymorphic di- and tri-nucleotide nuclear microsatellite markers were developed for Melaleuca nematophylla, the Wiry Honey Myrtle, a common shrub of southwest Western Australia. Populations are patchily distributed in a highly fragmented landscape due to extensive vegetation clearing for agriculture and population genetic structure is being investigated to inform appropriate seed collection and restoration strategies. Moderate diversity was observed in two populations with the number of alleles per locus ranging from 2 to 17. Expected and observed heterozygosity averaged 0.606 and 0.423, respectively. All loci showed independent inheritance but there was evidence of possible null alleles at some loci in each population. © 2014 @Crown copyright as represented by the state of Western Australia.

AB - A genomic library was constructed and 14 novel polymorphic di- and tri-nucleotide nuclear microsatellite markers were developed for Melaleuca nematophylla, the Wiry Honey Myrtle, a common shrub of southwest Western Australia. Populations are patchily distributed in a highly fragmented landscape due to extensive vegetation clearing for agriculture and population genetic structure is being investigated to inform appropriate seed collection and restoration strategies. Moderate diversity was observed in two populations with the number of alleles per locus ranging from 2 to 17. Expected and observed heterozygosity averaged 0.606 and 0.423, respectively. All loci showed independent inheritance but there was evidence of possible null alleles at some loci in each population. © 2014 @Crown copyright as represented by the state of Western Australia.

U2 - 10.1007/s12686-013-0118-z

DO - 10.1007/s12686-013-0118-z

M3 - Letter

SN - 1877-7252

VL - 6

SP - 439

EP - 441

JO - Conservation Genetics Resources

JF - Conservation Genetics Resources

IS - 2

ER -

Characterisation of microsatellite DNA markers for the Wiry Honey Myrtle, Melaleuca nematophylla Craven

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