Characterisation of microsatellite DNA markers for the Wiry Honey Myrtle, Melaleuca nematophylla Craven

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Abstract

A genomic library was constructed and 14 novel polymorphic di- and tri-nucleotide nuclear microsatellite markers were developed for Melaleuca nematophylla, the Wiry Honey Myrtle, a common shrub of southwest Western Australia. Populations are patchily distributed in a highly fragmented landscape due to extensive vegetation clearing for agriculture and population genetic structure is being investigated to inform appropriate seed collection and restoration strategies. Moderate diversity was observed in two populations with the number of alleles per locus ranging from 2 to 17. Expected and observed heterozygosity averaged 0.606 and 0.423, respectively. All loci showed independent inheritance but there was evidence of possible null alleles at some loci in each population. © 2014 @Crown copyright as represented by the state of Western Australia.
Original languageEnglish
Pages (from-to)439-441
Number of pages3
JournalConservation Genetics Resources
Volume6
Issue number2
Early online date11 Jan 2014
DOIs
Publication statusPublished - Jun 2014

Fingerprint

Myrtus
Melaleuca
Myrtus communis
Honey
honey
Genetic Markers
Microsatellite Repeats
allele
Western Australia
microsatellite repeats
seed collection
DNA
loci
genetic markers
Nucleotides
nucleotides
Alleles
heterozygosity
genetic structure
population genetics

Cite this

@article{bc975036d4ad45b38bd5907282a50242,
title = "Characterisation of microsatellite DNA markers for the Wiry Honey Myrtle, Melaleuca nematophylla Craven",
abstract = "A genomic library was constructed and 14 novel polymorphic di- and tri-nucleotide nuclear microsatellite markers were developed for Melaleuca nematophylla, the Wiry Honey Myrtle, a common shrub of southwest Western Australia. Populations are patchily distributed in a highly fragmented landscape due to extensive vegetation clearing for agriculture and population genetic structure is being investigated to inform appropriate seed collection and restoration strategies. Moderate diversity was observed in two populations with the number of alleles per locus ranging from 2 to 17. Expected and observed heterozygosity averaged 0.606 and 0.423, respectively. All loci showed independent inheritance but there was evidence of possible null alleles at some loci in each population. {\circledC} 2014 @Crown copyright as represented by the state of Western Australia.",
author = "Melissa Millar and Margaret Byrne and David Coates and Dale Roberts",
year = "2014",
month = "6",
doi = "10.1007/s12686-013-0118-z",
language = "English",
volume = "6",
pages = "439--441",
journal = "Conservation Genetics Resources",
issn = "1877-7252",
publisher = "Springer-Verlag London Ltd.",
number = "2",

}

TY - JOUR

T1 - Characterisation of microsatellite DNA markers for the Wiry Honey Myrtle, Melaleuca nematophylla Craven

AU - Millar, Melissa

AU - Byrne, Margaret

AU - Coates, David

AU - Roberts, Dale

PY - 2014/6

Y1 - 2014/6

N2 - A genomic library was constructed and 14 novel polymorphic di- and tri-nucleotide nuclear microsatellite markers were developed for Melaleuca nematophylla, the Wiry Honey Myrtle, a common shrub of southwest Western Australia. Populations are patchily distributed in a highly fragmented landscape due to extensive vegetation clearing for agriculture and population genetic structure is being investigated to inform appropriate seed collection and restoration strategies. Moderate diversity was observed in two populations with the number of alleles per locus ranging from 2 to 17. Expected and observed heterozygosity averaged 0.606 and 0.423, respectively. All loci showed independent inheritance but there was evidence of possible null alleles at some loci in each population. © 2014 @Crown copyright as represented by the state of Western Australia.

AB - A genomic library was constructed and 14 novel polymorphic di- and tri-nucleotide nuclear microsatellite markers were developed for Melaleuca nematophylla, the Wiry Honey Myrtle, a common shrub of southwest Western Australia. Populations are patchily distributed in a highly fragmented landscape due to extensive vegetation clearing for agriculture and population genetic structure is being investigated to inform appropriate seed collection and restoration strategies. Moderate diversity was observed in two populations with the number of alleles per locus ranging from 2 to 17. Expected and observed heterozygosity averaged 0.606 and 0.423, respectively. All loci showed independent inheritance but there was evidence of possible null alleles at some loci in each population. © 2014 @Crown copyright as represented by the state of Western Australia.

U2 - 10.1007/s12686-013-0118-z

DO - 10.1007/s12686-013-0118-z

M3 - Letter

VL - 6

SP - 439

EP - 441

JO - Conservation Genetics Resources

JF - Conservation Genetics Resources

SN - 1877-7252

IS - 2

ER -