Characterisation of microsatellite DNA markers for Mirbelia bursarioides A.M. Monro and Crisp ms

MA Millar; M Byrne; DJ Coates; John Roberts

doi:10.1007/s12686-014-0184-x

Characterisation of microsatellite DNA markers for Mirbelia bursarioides A.M. Monro and Crisp ms

MA Millar, M Byrne, DJ Coates, John Roberts

School of Biological Sciences

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Abstract

A genomic library was constructed and 12 novel polymorphic di- and tri-nucleotide nuclear microsatellite markers were developed for Mirbelia bursarioides (Fabaceae), a shrub from the semiarid zone of southwest Western Australia. Populations are patchily distributed in a highly fragmented landscape. Genetic diversity in a single population was high with the number of alleles per locus ranging from 4 to 12. Expected and observed heterozygosities averaged 0.686 and 0.720 respectively. All loci showed independent inheritance. One locus showed possible evidence of null alleles. Population genetic structure is being investigated to inform appropriate seed collection and restoration strategies.

Original language	English
Pages (from-to)	693-695
Number of pages	3
Journal	Conservation Genetics Resources
Volume	6
Issue number	3
Early online date	5 Apr 2014
DOIs	https://doi.org/10.1007/s12686-014-0184-x
Publication status	Published - Sep 2014

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title = "Characterisation of microsatellite DNA markers for Mirbelia bursarioides A.M. Monro and Crisp ms",

abstract = "A genomic library was constructed and 12 novel polymorphic di- and tri-nucleotide nuclear microsatellite markers were developed for Mirbelia bursarioides (Fabaceae), a shrub from the semiarid zone of southwest Western Australia. Populations are patchily distributed in a highly fragmented landscape. Genetic diversity in a single population was high with the number of alleles per locus ranging from 4 to 12. Expected and observed heterozygosities averaged 0.686 and 0.720 respectively. All loci showed independent inheritance. One locus showed possible evidence of null alleles. Population genetic structure is being investigated to inform appropriate seed collection and restoration strategies.",

author = "MA Millar and M Byrne and DJ Coates and John Roberts",

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doi = "10.1007/s12686-014-0184-x",

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AU - Millar, MA

AU - Byrne, M

AU - Coates, DJ

AU - Roberts, John

PY - 2014/9

Y1 - 2014/9

N2 - A genomic library was constructed and 12 novel polymorphic di- and tri-nucleotide nuclear microsatellite markers were developed for Mirbelia bursarioides (Fabaceae), a shrub from the semiarid zone of southwest Western Australia. Populations are patchily distributed in a highly fragmented landscape. Genetic diversity in a single population was high with the number of alleles per locus ranging from 4 to 12. Expected and observed heterozygosities averaged 0.686 and 0.720 respectively. All loci showed independent inheritance. One locus showed possible evidence of null alleles. Population genetic structure is being investigated to inform appropriate seed collection and restoration strategies.

AB - A genomic library was constructed and 12 novel polymorphic di- and tri-nucleotide nuclear microsatellite markers were developed for Mirbelia bursarioides (Fabaceae), a shrub from the semiarid zone of southwest Western Australia. Populations are patchily distributed in a highly fragmented landscape. Genetic diversity in a single population was high with the number of alleles per locus ranging from 4 to 12. Expected and observed heterozygosities averaged 0.686 and 0.720 respectively. All loci showed independent inheritance. One locus showed possible evidence of null alleles. Population genetic structure is being investigated to inform appropriate seed collection and restoration strategies.

U2 - 10.1007/s12686-014-0184-x

DO - 10.1007/s12686-014-0184-x

M3 - Comment/debate

VL - 6

SP - 693

EP - 695

JO - Conservation Genetics Resources

JF - Conservation Genetics Resources

SN - 1877-7252

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ER -