A genomic library was constructed and 12 novel polymorphic di- and tri-nucleotide nuclear microsatellite markers were developed for Mirbelia bursarioides (Fabaceae), a shrub from the semiarid zone of southwest Western Australia. Populations are patchily distributed in a highly fragmented landscape. Genetic diversity in a single population was high with the number of alleles per locus ranging from 4 to 12. Expected and observed heterozygosities averaged 0.686 and 0.720 respectively. All loci showed independent inheritance. One locus showed possible evidence of null alleles. Population genetic structure is being investigated to inform appropriate seed collection and restoration strategies.