Characterisation of microsatellite DNA markers for Mirbelia bursarioides A.M. Monro and Crisp ms

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Abstract

A genomic library was constructed and 12 novel polymorphic di- and tri-nucleotide nuclear microsatellite markers were developed for Mirbelia bursarioides (Fabaceae), a shrub from the semiarid zone of southwest Western Australia. Populations are patchily distributed in a highly fragmented landscape. Genetic diversity in a single population was high with the number of alleles per locus ranging from 4 to 12. Expected and observed heterozygosities averaged 0.686 and 0.720 respectively. All loci showed independent inheritance. One locus showed possible evidence of null alleles. Population genetic structure is being investigated to inform appropriate seed collection and restoration strategies.
Original languageEnglish
Pages (from-to)693-695
Number of pages3
JournalConservation Genetics Resources
Volume6
Issue number3
Early online date5 Apr 2014
DOIs
Publication statusPublished - Sep 2014

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