Characterisation of microsatellite DNA markers for Grevillea paradoxa (F. Muell)

Melissa Millar; Margaret Byrne; David Coates; Dale Roberts

doi:10.1007/s12686-013-0027-1

Characterisation of microsatellite DNA markers for Grevillea paradoxa (F. Muell)

Melissa Millar, Margaret Byrne, David Coates, Dale Roberts

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Abstract

A genomic library was constructed and 14 novel polymorphic di- and tri-nucleotide nuclear microsatellite markers were developed for Grevillea paradoxa, the Bottlebrush Grevillea, a common shrub in southwest Western Australia. Populations are patchily distributed in a highly fragmented landscape due to extensive vegetation clearing for agriculture and population genetic structure is being investigated to inform appropriate seed collection and restoration strategies. Moderate diversity was observed in two populations with the number of alleles per locus ranging from 1 to 9. Expected and observed heterozygosities averaged 0.434 and 0.344 respectively. All loci showed independent inheritance but there was evidence of possible null alleles at some loci in each population.

Original language	English
Pages (from-to)	139-141
Journal	Conservation Genetics Resources
Volume	6
Issue number	1
DOIs	https://doi.org/10.1007/s12686-013-0027-1
Publication status	Published - Mar 2014

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title = "Characterisation of microsatellite DNA markers for Grevillea paradoxa (F. Muell)",

abstract = "A genomic library was constructed and 14 novel polymorphic di- and tri-nucleotide nuclear microsatellite markers were developed for Grevillea paradoxa, the Bottlebrush Grevillea, a common shrub in southwest Western Australia. Populations are patchily distributed in a highly fragmented landscape due to extensive vegetation clearing for agriculture and population genetic structure is being investigated to inform appropriate seed collection and restoration strategies. Moderate diversity was observed in two populations with the number of alleles per locus ranging from 1 to 9. Expected and observed heterozygosities averaged 0.434 and 0.344 respectively. All loci showed independent inheritance but there was evidence of possible null alleles at some loci in each population.",

author = "Melissa Millar and Margaret Byrne and David Coates and Dale Roberts",

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AU - Millar, Melissa

AU - Byrne, Margaret

AU - Coates, David

AU - Roberts, Dale

PY - 2014/3

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N2 - A genomic library was constructed and 14 novel polymorphic di- and tri-nucleotide nuclear microsatellite markers were developed for Grevillea paradoxa, the Bottlebrush Grevillea, a common shrub in southwest Western Australia. Populations are patchily distributed in a highly fragmented landscape due to extensive vegetation clearing for agriculture and population genetic structure is being investigated to inform appropriate seed collection and restoration strategies. Moderate diversity was observed in two populations with the number of alleles per locus ranging from 1 to 9. Expected and observed heterozygosities averaged 0.434 and 0.344 respectively. All loci showed independent inheritance but there was evidence of possible null alleles at some loci in each population.

AB - A genomic library was constructed and 14 novel polymorphic di- and tri-nucleotide nuclear microsatellite markers were developed for Grevillea paradoxa, the Bottlebrush Grevillea, a common shrub in southwest Western Australia. Populations are patchily distributed in a highly fragmented landscape due to extensive vegetation clearing for agriculture and population genetic structure is being investigated to inform appropriate seed collection and restoration strategies. Moderate diversity was observed in two populations with the number of alleles per locus ranging from 1 to 9. Expected and observed heterozygosities averaged 0.434 and 0.344 respectively. All loci showed independent inheritance but there was evidence of possible null alleles at some loci in each population.

U2 - 10.1007/s12686-013-0027-1

DO - 10.1007/s12686-013-0027-1

M3 - Letter

VL - 6

SP - 139

EP - 141

JO - Conservation Genetics Resources

JF - Conservation Genetics Resources

SN - 1877-7252

IS - 1

ER -