Characterisation of microsatellite DNA markers for Grevillea paradoxa (F. Muell)

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    Abstract

    A genomic library was constructed and 14 novel polymorphic di- and tri-nucleotide nuclear microsatellite markers were developed for Grevillea paradoxa, the Bottlebrush Grevillea, a common shrub in southwest Western Australia. Populations are patchily distributed in a highly fragmented landscape due to extensive vegetation clearing for agriculture and population genetic structure is being investigated to inform appropriate seed collection and restoration strategies. Moderate diversity was observed in two populations with the number of alleles per locus ranging from 1 to 9. Expected and observed heterozygosities averaged 0.434 and 0.344 respectively. All loci showed independent inheritance but there was evidence of possible null alleles at some loci in each population.
    Original languageEnglish
    Pages (from-to)139-141
    JournalConservation Genetics Resources
    Volume6
    Issue number1
    DOIs
    Publication statusPublished - Mar 2014

    Fingerprint

    Proteaceae
    Grevillea
    Genetic Markers
    Microsatellite Repeats
    allele
    microsatellite repeats
    seed collection
    DNA
    loci
    genetic markers
    Nucleotides
    nucleotides
    Alleles
    heterozygosity
    genetic structure
    population genetics
    Population
    genomics
    Western Australia
    shrub

    Cite this

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    title = "Characterisation of microsatellite DNA markers for Grevillea paradoxa (F. Muell)",
    abstract = "A genomic library was constructed and 14 novel polymorphic di- and tri-nucleotide nuclear microsatellite markers were developed for Grevillea paradoxa, the Bottlebrush Grevillea, a common shrub in southwest Western Australia. Populations are patchily distributed in a highly fragmented landscape due to extensive vegetation clearing for agriculture and population genetic structure is being investigated to inform appropriate seed collection and restoration strategies. Moderate diversity was observed in two populations with the number of alleles per locus ranging from 1 to 9. Expected and observed heterozygosities averaged 0.434 and 0.344 respectively. All loci showed independent inheritance but there was evidence of possible null alleles at some loci in each population.",
    author = "Melissa Millar and Margaret Byrne and David Coates and Dale Roberts",
    year = "2014",
    month = "3",
    doi = "10.1007/s12686-013-0027-1",
    language = "English",
    volume = "6",
    pages = "139--141",
    journal = "Conservation Genetics Resources",
    issn = "1877-7252",
    publisher = "Springer-Verlag London Ltd.",
    number = "1",

    }

    TY - JOUR

    T1 - Characterisation of microsatellite DNA markers for Grevillea paradoxa (F. Muell)

    AU - Millar, Melissa

    AU - Byrne, Margaret

    AU - Coates, David

    AU - Roberts, Dale

    PY - 2014/3

    Y1 - 2014/3

    N2 - A genomic library was constructed and 14 novel polymorphic di- and tri-nucleotide nuclear microsatellite markers were developed for Grevillea paradoxa, the Bottlebrush Grevillea, a common shrub in southwest Western Australia. Populations are patchily distributed in a highly fragmented landscape due to extensive vegetation clearing for agriculture and population genetic structure is being investigated to inform appropriate seed collection and restoration strategies. Moderate diversity was observed in two populations with the number of alleles per locus ranging from 1 to 9. Expected and observed heterozygosities averaged 0.434 and 0.344 respectively. All loci showed independent inheritance but there was evidence of possible null alleles at some loci in each population.

    AB - A genomic library was constructed and 14 novel polymorphic di- and tri-nucleotide nuclear microsatellite markers were developed for Grevillea paradoxa, the Bottlebrush Grevillea, a common shrub in southwest Western Australia. Populations are patchily distributed in a highly fragmented landscape due to extensive vegetation clearing for agriculture and population genetic structure is being investigated to inform appropriate seed collection and restoration strategies. Moderate diversity was observed in two populations with the number of alleles per locus ranging from 1 to 9. Expected and observed heterozygosities averaged 0.434 and 0.344 respectively. All loci showed independent inheritance but there was evidence of possible null alleles at some loci in each population.

    U2 - 10.1007/s12686-013-0027-1

    DO - 10.1007/s12686-013-0027-1

    M3 - Letter

    VL - 6

    SP - 139

    EP - 141

    JO - Conservation Genetics Resources

    JF - Conservation Genetics Resources

    SN - 1877-7252

    IS - 1

    ER -