Characterisation of microsatellite DNA markers for Grevillea globosa C. A. Gardner

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    Abstract

    A genomic library was constructed and 14 novel polymorphic di- and tri-nucleotide nuclear microsatellite markers were developed for Grevillea globosa, an endemic shrub of southwest Western Australia. Populations are patchily distributed and population genetic structure is being investigated to inform appropriate seed collection and restoration strategies. Diversity in a selected population was high, with the number of alleles per locus ranging from 3 to 13 and expected and observed heterozygosities averaging 0.693 and 0.799 respectively. All loci showed independent inheritance and there was no evidence of possible null alleles.
    Original languageEnglish
    Pages (from-to)689-691
    JournalConservation Genetics Resources
    Volume6
    Issue number3
    Early online date5 Apr 2014
    DOIs
    Publication statusPublished - Sep 2014

    Fingerprint

    Proteaceae
    Grevillea
    Genetic Markers
    Microsatellite Repeats
    allele
    Nucleotides
    nucleotides
    Alleles
    microsatellite repeats
    seed collection
    DNA
    Western Australia
    loci
    genetic markers
    Genomic Library
    null alleles
    Genetic Structures
    genomic libraries
    Population Genetics
    heterozygosity

    Cite this

    @article{190b230776e340c2be423f9f33ce7a16,
    title = "Characterisation of microsatellite DNA markers for Grevillea globosa C. A. Gardner",
    abstract = "A genomic library was constructed and 14 novel polymorphic di- and tri-nucleotide nuclear microsatellite markers were developed for Grevillea globosa, an endemic shrub of southwest Western Australia. Populations are patchily distributed and population genetic structure is being investigated to inform appropriate seed collection and restoration strategies. Diversity in a selected population was high, with the number of alleles per locus ranging from 3 to 13 and expected and observed heterozygosities averaging 0.693 and 0.799 respectively. All loci showed independent inheritance and there was no evidence of possible null alleles.",
    author = "Melissa Millar and Margaret Byrne and David Coates and Dale Roberts",
    year = "2014",
    month = "9",
    doi = "10.1007/s12686-014-0183-y",
    language = "English",
    volume = "6",
    pages = "689--691",
    journal = "Conservation Genetics Resources",
    issn = "1877-7252",
    publisher = "Springer-Verlag London Ltd.",
    number = "3",

    }

    TY - JOUR

    T1 - Characterisation of microsatellite DNA markers for Grevillea globosa C. A. Gardner

    AU - Millar, Melissa

    AU - Byrne, Margaret

    AU - Coates, David

    AU - Roberts, Dale

    PY - 2014/9

    Y1 - 2014/9

    N2 - A genomic library was constructed and 14 novel polymorphic di- and tri-nucleotide nuclear microsatellite markers were developed for Grevillea globosa, an endemic shrub of southwest Western Australia. Populations are patchily distributed and population genetic structure is being investigated to inform appropriate seed collection and restoration strategies. Diversity in a selected population was high, with the number of alleles per locus ranging from 3 to 13 and expected and observed heterozygosities averaging 0.693 and 0.799 respectively. All loci showed independent inheritance and there was no evidence of possible null alleles.

    AB - A genomic library was constructed and 14 novel polymorphic di- and tri-nucleotide nuclear microsatellite markers were developed for Grevillea globosa, an endemic shrub of southwest Western Australia. Populations are patchily distributed and population genetic structure is being investigated to inform appropriate seed collection and restoration strategies. Diversity in a selected population was high, with the number of alleles per locus ranging from 3 to 13 and expected and observed heterozygosities averaging 0.693 and 0.799 respectively. All loci showed independent inheritance and there was no evidence of possible null alleles.

    U2 - 10.1007/s12686-014-0183-y

    DO - 10.1007/s12686-014-0183-y

    M3 - Letter

    VL - 6

    SP - 689

    EP - 691

    JO - Conservation Genetics Resources

    JF - Conservation Genetics Resources

    SN - 1877-7252

    IS - 3

    ER -