Characterisation of microsatellite DNA markers for Grevillea globosa C. A. Gardner

Melissa Millar; Margaret Byrne; David Coates; Dale Roberts

doi:10.1007/s12686-014-0183-y

Characterisation of microsatellite DNA markers for Grevillea globosa C. A. Gardner

Melissa Millar, Margaret Byrne, David Coates, Dale Roberts

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Abstract

A genomic library was constructed and 14 novel polymorphic di- and tri-nucleotide nuclear microsatellite markers were developed for Grevillea globosa, an endemic shrub of southwest Western Australia. Populations are patchily distributed and population genetic structure is being investigated to inform appropriate seed collection and restoration strategies. Diversity in a selected population was high, with the number of alleles per locus ranging from 3 to 13 and expected and observed heterozygosities averaging 0.693 and 0.799 respectively. All loci showed independent inheritance and there was no evidence of possible null alleles.

Original language	English
Pages (from-to)	689-691
Journal	Conservation Genetics Resources
Volume	6
Issue number	3
Early online date	5 Apr 2014
DOIs	https://doi.org/10.1007/s12686-014-0183-y
Publication status	Published - Sept 2014

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Managing Genetic Diversity & Evolutionary Processes in Foundation Species for Landscape Restoration in the Midwest of Western Australia
Roberts, D., Coates, D. & Byrne, M.
ARC Australian Research Council
1/01/12 → 31/12/14
Project: Research

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abstract = "A genomic library was constructed and 14 novel polymorphic di- and tri-nucleotide nuclear microsatellite markers were developed for Grevillea globosa, an endemic shrub of southwest Western Australia. Populations are patchily distributed and population genetic structure is being investigated to inform appropriate seed collection and restoration strategies. Diversity in a selected population was high, with the number of alleles per locus ranging from 3 to 13 and expected and observed heterozygosities averaging 0.693 and 0.799 respectively. All loci showed independent inheritance and there was no evidence of possible null alleles. ",

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AB - A genomic library was constructed and 14 novel polymorphic di- and tri-nucleotide nuclear microsatellite markers were developed for Grevillea globosa, an endemic shrub of southwest Western Australia. Populations are patchily distributed and population genetic structure is being investigated to inform appropriate seed collection and restoration strategies. Diversity in a selected population was high, with the number of alleles per locus ranging from 3 to 13 and expected and observed heterozygosities averaging 0.693 and 0.799 respectively. All loci showed independent inheritance and there was no evidence of possible null alleles.

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Characterisation of microsatellite DNA markers for Grevillea globosa C. A. Gardner

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Managing Genetic Diversity & Evolutionary Processes in Foundation Species for Landscape Restoration in the Midwest of Western Australia

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