Characterisation of hyaluronic acid methylcellulose hydrogels for 3D bioprinting

Nicholas Law, Brandon Doney, Hayley Glover, Yahua Qin, Zachary M. Aman, Timothy B. Sercombe, Lawrence J. Liew, Rodney J. Dilley, Barry J. Doyle

Research output: Contribution to journalArticle

  • 2 Citations

Abstract

Hydrogels containing hyaluronic acid (HA) and methylcellulose (MC) have shown promising results for three dimensional (3D) bioprinting applications. However, several parameters influence the applicability bioprinting and there is scarce data in the literature characterising HAMC. We assessed eight concentrations of HAMC for printability, swelling and stability over time, rheological and structural behaviour, and viability of mesenchymal stem cells. We show that HAMC blends behave as viscous solutions at 4 °C and have faster gelation times at higher temperatures, typically gelling upon reaching 37 °C. We found the storage, loss and compressive moduli to be dependent on HAMC concentration and incubation time at 37 °C, and show the compressive modulus to be strain-rate dependent. Swelling and stability was influenced by time, more so than pH environment. We demonstrated that mesenchymal stem cell viability was above 75% in bioprinted structures and cells remain viable for at least one week after 3D bioprinting. The mechanical properties of HAMC are highly tuneable and we show that higher concentrations of HAMC are particularly suited to cell-encapsulated 3D bioprinting applications that require scaffold structure and delivery of cells.

LanguageEnglish
Pages389-399
Number of pages11
JournalJournal of the Mechanical Behavior of Biomedical Materials
Volume77
DOIs
StatePublished - 1 Jan 2018

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Hyaluronic acid
Hydrogels
Methylcellulose
Hyaluronic Acid
Stem cells
Swelling
Gelation
Scaffolds
Strain rate
Mechanical properties
Temperature

Cite this

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title = "Characterisation of hyaluronic acid methylcellulose hydrogels for 3D bioprinting",
abstract = "Hydrogels containing hyaluronic acid (HA) and methylcellulose (MC) have shown promising results for three dimensional (3D) bioprinting applications. However, several parameters influence the applicability bioprinting and there is scarce data in the literature characterising HAMC. We assessed eight concentrations of HAMC for printability, swelling and stability over time, rheological and structural behaviour, and viability of mesenchymal stem cells. We show that HAMC blends behave as viscous solutions at 4 °C and have faster gelation times at higher temperatures, typically gelling upon reaching 37 °C. We found the storage, loss and compressive moduli to be dependent on HAMC concentration and incubation time at 37 °C, and show the compressive modulus to be strain-rate dependent. Swelling and stability was influenced by time, more so than pH environment. We demonstrated that mesenchymal stem cell viability was above 75{\%} in bioprinted structures and cells remain viable for at least one week after 3D bioprinting. The mechanical properties of HAMC are highly tuneable and we show that higher concentrations of HAMC are particularly suited to cell-encapsulated 3D bioprinting applications that require scaffold structure and delivery of cells.",
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author = "Nicholas Law and Brandon Doney and Hayley Glover and Yahua Qin and Aman, {Zachary M.} and Sercombe, {Timothy B.} and Liew, {Lawrence J.} and Dilley, {Rodney J.} and Doyle, {Barry J.}",
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Characterisation of hyaluronic acid methylcellulose hydrogels for 3D bioprinting. / Law, Nicholas; Doney, Brandon; Glover, Hayley; Qin, Yahua; Aman, Zachary M.; Sercombe, Timothy B.; Liew, Lawrence J.; Dilley, Rodney J.; Doyle, Barry J.

In: Journal of the Mechanical Behavior of Biomedical Materials, Vol. 77, 01.01.2018, p. 389-399.

Research output: Contribution to journalArticle

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T1 - Characterisation of hyaluronic acid methylcellulose hydrogels for 3D bioprinting

AU - Law,Nicholas

AU - Doney,Brandon

AU - Glover,Hayley

AU - Qin,Yahua

AU - Aman,Zachary M.

AU - Sercombe,Timothy B.

AU - Liew,Lawrence J.

AU - Dilley,Rodney J.

AU - Doyle,Barry J.

PY - 2018/1/1

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KW - Bioprinting

KW - Characterisation

KW - Hyaluronic acid

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