Characterisation of genetic diversity and DNA fingerprinting of Australian chickpea (Cicer arietinum L.) cultivars using MFLP markers

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Abstract

Chickpea (Cicer arietinum L.) is one of the major grain legume crops in the world. In this study, the genetic diversity of 24 Australian chickpea cultivars released between 1987 and 2005 was investigated with microsatellite- anchored fragment length polymorphism (MFLP) DNA markers. Among the cultivars examined, 30 cultivar-specific markers were identi. ed and all were unequivocally identi. ed using theDNA. ngerprints developed in this study. Most of the cultivars were grouped into two major clusters; cv. Flipper was separated from the rest based on total character differences of DNA polymorphism. TheMFLPapproach proved suitable in the analysis of genetic diversity among the chickpea cultivars studied and the genetic relationship identi. ed will be useful for chickpea breeding programs in selecting parent materials.
Original languageEnglish
Pages (from-to)707-713
JournalAustralian Journal of Agricultural Research
Volume59
DOIs
Publication statusPublished - 2008

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Cicer arietinum
DNA fingerprinting
polymorphism
genetic polymorphism
microsatellite repeats
genetic variation
cultivars
genetic relationships
legumes
genetic markers
breeding
DNA
crops

Cite this

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title = "Characterisation of genetic diversity and DNA fingerprinting of Australian chickpea (Cicer arietinum L.) cultivars using MFLP markers",
abstract = "Chickpea (Cicer arietinum L.) is one of the major grain legume crops in the world. In this study, the genetic diversity of 24 Australian chickpea cultivars released between 1987 and 2005 was investigated with microsatellite- anchored fragment length polymorphism (MFLP) DNA markers. Among the cultivars examined, 30 cultivar-specific markers were identi. ed and all were unequivocally identi. ed using theDNA. ngerprints developed in this study. Most of the cultivars were grouped into two major clusters; cv. Flipper was separated from the rest based on total character differences of DNA polymorphism. TheMFLPapproach proved suitable in the analysis of genetic diversity among the chickpea cultivars studied and the genetic relationship identi. ed will be useful for chickpea breeding programs in selecting parent materials.",
author = "R. Lin and H. Yang and Tanveer Khan and Kadambot Siddique and Guijun Yan",
year = "2008",
doi = "10.1071/AR07401",
language = "English",
volume = "59",
pages = "707--713",
journal = "Crop & Pasture Science",
issn = "1836-0947",
publisher = "CSIRO Publishing",

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TY - JOUR

T1 - Characterisation of genetic diversity and DNA fingerprinting of Australian chickpea (Cicer arietinum L.) cultivars using MFLP markers

AU - Lin, R.

AU - Yang, H.

AU - Khan, Tanveer

AU - Siddique, Kadambot

AU - Yan, Guijun

PY - 2008

Y1 - 2008

N2 - Chickpea (Cicer arietinum L.) is one of the major grain legume crops in the world. In this study, the genetic diversity of 24 Australian chickpea cultivars released between 1987 and 2005 was investigated with microsatellite- anchored fragment length polymorphism (MFLP) DNA markers. Among the cultivars examined, 30 cultivar-specific markers were identi. ed and all were unequivocally identi. ed using theDNA. ngerprints developed in this study. Most of the cultivars were grouped into two major clusters; cv. Flipper was separated from the rest based on total character differences of DNA polymorphism. TheMFLPapproach proved suitable in the analysis of genetic diversity among the chickpea cultivars studied and the genetic relationship identi. ed will be useful for chickpea breeding programs in selecting parent materials.

AB - Chickpea (Cicer arietinum L.) is one of the major grain legume crops in the world. In this study, the genetic diversity of 24 Australian chickpea cultivars released between 1987 and 2005 was investigated with microsatellite- anchored fragment length polymorphism (MFLP) DNA markers. Among the cultivars examined, 30 cultivar-specific markers were identi. ed and all were unequivocally identi. ed using theDNA. ngerprints developed in this study. Most of the cultivars were grouped into two major clusters; cv. Flipper was separated from the rest based on total character differences of DNA polymorphism. TheMFLPapproach proved suitable in the analysis of genetic diversity among the chickpea cultivars studied and the genetic relationship identi. ed will be useful for chickpea breeding programs in selecting parent materials.

U2 - 10.1071/AR07401

DO - 10.1071/AR07401

M3 - Article

VL - 59

SP - 707

EP - 713

JO - Crop & Pasture Science

JF - Crop & Pasture Science

SN - 1836-0947

ER -